Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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A Tandem Water and Hexane Washing Method for Economical Recovery of Paclitaxel from Biomass

바이오매스 유래 파클리탁셀의 경제적인 회수를 위한 물과 헥산의 순차적 세척 방법

  • Lee, Myeong-Gi (Department of Chemical Engineering, Kongju National University) ;
  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 이명기 (공주대학교 화학공학부) ;
  • 김진현 (공주대학교 화학공학부)
  • Received : 2021.06.17
  • Accepted : 2021.08.30
  • Published : 2022.02.01
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Abstract

In this study, a tandem water and hexane washing process was developed to improve the recovery efficiency of paclitaxel derived from Taxus chinensis. The polar impurities contained in the sample were effectively removed by washing with water at a sample/water ratio of 1:40 (w/v) for 10 min. In addition, the non-polar impurities were effectively removed by washing with hexane at a sample/hexane ratio of 1:160 (w/v) for 20 min. A high purity of paclitaxel (>30.0%) was obtained in a short operating time (~30 min) by sequential washing with water and hexane.

본 연구에서는 Taxus chinensis 유래 파클리탁셀의 회수 효율을 개선하기 위하여, 물 및 헥산의 순차적 세척 공정을 개발하였다. 물 세척은 시료/물 비 1:40 (w/v)에서 10 분 동안 세척함으로써 시료에 포함되어 있는 극성불순물을 효과적으로 제거할 수 있었다. 헥산 세척은 시료/헥산 비 1:160 (w/v)에서 20 분 동안 세척함으로써 시료에 포함되어 있는 비극성 불순물을 효과적으로 제거할 수 있었다. 물과 헥산의 순차적 세척을 통하여 비교적 짧은 공정 시간(~30 분)에 파클리탁셀 순도 30% 이상을 얻을 수 있었다.

Keywords

Acknowledgement

이 성과는 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(과제번호: NRF-2021R1A2C1003186).

References

  1. Park, J. M. and Kim, J. H., "Ultrasound-assisted Micellar Extraction for Paclitaxel Purification from Taxus chinensis," Korean Chem. Eng. Res., 59, 106-111(2021).
  2. Zhu, L. and Chen, L., "Progress in Research on Paclitaxel and Tumor Immunotherapy," Cell. Mol. Biol. Lett., 24, 40(2019). https://doi.org/10.1186/s11658-019-0164-y
  3. Tan, Z., Li, Q., Wang, C., Zhou, W., Yang, Y., Wang, H., Yi, Y. and Li, F., "Ultrasonic Assisted Extraction of Paclitaxel from Taxus x media Using Ionic Liquids as Adjuvants: Optimization of the Process by Response Surface Methodology," Molecules, 22, 1483(2017). https://doi.org/10.3390/molecules22091483
  4. Wei, Y., Pu, X. and Zhao, L., "Preclinical Studies for the Combination of Paclitaxel and Curcumin in Cancer Therapy," Oncol. Rep., 37, 3159-3166(2017). https://doi.org/10.3892/or.2017.5593
  5. Zu, Y., Wang, Y., Fu, Y., Li, S., Sun, R., Liu, W. and Luo, H., "Enzyme-assisted Extraction pf Paclitaxel and Related Taxanes From Needles of Taxus chinensis," Sep. Purif. Technol., 68, 238-243(2009). https://doi.org/10.1016/j.seppur.2009.05.009
  6. Oguzkan, S. B., Karagul, B., Uzun, A., Guler, O. O. and Ugras, H. I., "Pre-purification of An Anticancer Drug (paclitaxel) Obtained from Nut Husks," Int. J. Pharmacol., 14, 76-82(2018). https://doi.org/10.3923/ijp.2018.76.82
  7. Ochoa-Villarreal, M., Howat, S., Hong, S., Jang, M. O., Jin, Y.-W., Lee, E.-K. and Loake, G. J., "Plant Cell Culture Strategies for the Production of Natural Products," BMB Rep., 49, 149-158(2016). https://doi.org/10.5483/BMBRep.2016.49.3.264
  8. Pyo, S. H., Park, H. B., Song, B. K., Han, B. H. and Kim, J. H., "A Large-scale Purification of Paclitaxel from Cell Cultures of Taxus chinensis," Process Biochem., 39, 1985-1991(2004). https://doi.org/10.1016/j.procbio.2003.09.028
  9. McPartland, T. J., Patil, R. A., Malone, M. F. and Roberts, S. C., "Liquid-liquid Extraction for Recovery of Paclitaxel from Plant Cell Culture: Solvent Evaluation and Use of Extractants for Partitioning and Selectivity," Biotechnol. Prog., 28, 990-997(2012). https://doi.org/10.1002/btpr.1562
  10. Kang, H. J. and Kim, J. H., "Adsorption Kinetics, Mechanism, Isotherm, and Thermodynamic Analysis of Paclitaxel from Extracts of Taxus chinensis Cell Cultures Onto Sylopute," Biotechnol. Bioprocess Eng., 24, 513-521(2019). https://doi.org/10.1007/s12257-019-0001-1
  11. Oh, S. R. and Kim, J. H., "Ultrasound-based Fractional Precipitation for the Purification of (+)-dihydromyricetin," Korean J. Chem. Eng., 38, 480-484(2021). https://doi.org/10.1007/s11814-020-0734-8
  12. Lee, C. G. and Kim, J. H., "A Kinetic and Thermodynamic Study of Fractional Precipitation of Paclitaxel from Taxus chinensis," Process Biochem., 59, 216-222(2017). https://doi.org/10.1016/j.procbio.2017.05.016
  13. Lee, C. G. and Kim, J. H., "Increasing the Efficiency of the Separation and Purification Process for Paclitaxel by Pre-treatment with Water," Process Biochem., 51, 1738-1743(2016). https://doi.org/10.1016/j.procbio.2016.07.025
  14. Panchal, B., Deshmukh, S. and Sharma, M., "Optimization of Oil Extraction and Characterization from Tamarindus indica Linn Seed Oil," Int. J. Oil, Gas Coal Eng., 2, 1-6(2016). https://doi.org/10.11648/j.ogce.20140201.11