Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Anti-melanogenic property of ginsenoside Rf from Panax ginseng via inhibition of CREB/MITF pathway in melanocytes and ex vivo human skin

  • Lee, Ha-Ri (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Jung, Joon Min (Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Seo, Ji-Yeon (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Chang, Sung Eun (Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Song, Youngsup (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine)
  • Received : 2020.06.26
  • Accepted : 2020.11.16
  • Published : 2021.09.30
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Abstract

Background: Ginsenosides of Panax ginseng are used to enhance skin health and beauty. The present study aimed to investigate the potential use of ginsenoside Rf (Rf) from Panax ginseng as a new anti-pigmentation agent. Methods: The anti-melanogenic effects of Rf were explored. The transcriptional activity of the cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) and the expression levels of tyrosinase, microphthalmia-associated transcription factor (MITF), and tyrosinase-related proteins (Tyrps) were evaluated in melanocytes and UV-irradiated ex vivo human skin. Results: Rf significantly inhibited Forskolin (FSK) or UV-stimulated melanogenesis. Consistently, cellular tyrosinase activity and levels of MITF, tyrosinase, and Tyrps were downregulated. Furthermore, Rf suppressed MITF promoter activity, which was stimulated by FSK or CREB-regulated transcription coactivator 3 (CRTC3) overexpression. Increased CREB phosphorylation and protein kinase A (PKA) activity induced by FSK were also mitigated in the presence of Rf. Conclusion: Rf can be used as a reliable anti-pigmentation agent, which has a scientifically confirmed and reproducible action mechanism, via inhibition of CREB/MITF pathway.

Keywords

Acknowledgement

We thank Dr. Jin-Hwan Oh and Yul Hur for providing ginsenoside Rf, Dr. Montminy at the Salk Institute for Biological Studies for providing sera antibodies to CREB and phospho-CREB, and Professor Park at Department of Dermatology, Seoul National University, Bundang Hospital, Seongnam, Korea for providing Mel-Ab cells.

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