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http://dx.doi.org/10.1016/j.jgr.2020.11.003

Anti-melanogenic property of ginsenoside Rf from Panax ginseng via inhibition of CREB/MITF pathway in melanocytes and ex vivo human skin  

Lee, Ha-Ri (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine)
Jung, Joon Min (Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine)
Seo, Ji-Yeon (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine)
Chang, Sung Eun (Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine)
Song, Youngsup (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine)
Publication Information
Journal of Ginseng Research / v.45, no.5, 2021 , pp. 555-564 More about this Journal
Abstract
Background: Ginsenosides of Panax ginseng are used to enhance skin health and beauty. The present study aimed to investigate the potential use of ginsenoside Rf (Rf) from Panax ginseng as a new anti-pigmentation agent. Methods: The anti-melanogenic effects of Rf were explored. The transcriptional activity of the cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) and the expression levels of tyrosinase, microphthalmia-associated transcription factor (MITF), and tyrosinase-related proteins (Tyrps) were evaluated in melanocytes and UV-irradiated ex vivo human skin. Results: Rf significantly inhibited Forskolin (FSK) or UV-stimulated melanogenesis. Consistently, cellular tyrosinase activity and levels of MITF, tyrosinase, and Tyrps were downregulated. Furthermore, Rf suppressed MITF promoter activity, which was stimulated by FSK or CREB-regulated transcription coactivator 3 (CRTC3) overexpression. Increased CREB phosphorylation and protein kinase A (PKA) activity induced by FSK were also mitigated in the presence of Rf. Conclusion: Rf can be used as a reliable anti-pigmentation agent, which has a scientifically confirmed and reproducible action mechanism, via inhibition of CREB/MITF pathway.
Keywords
Panax ginseng; anti-pigmentation agent; CREB/MITF pathway; ginsenoside Rf;
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