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미숙과 복분자 추출물의 상체비만 개선 효과: 무작위배정, 이중눈가림, 위약-대조 임상연구

Effects of Unripe Rubus Coreanus Extract on Upper Body Obesity: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

  • 하기찬 (헬스케어크레임스엔드멘내지멘트(주)) ;
  • 김혜미 (헬스케어크레임스엔드멘내지멘트(주)) ;
  • 백향임 (헬스케어크레임스엔드멘내지멘트(주)) ;
  • 정다영 (헬스케어크레임스엔드멘내지멘트(주)) ;
  • 김은 ((주)비엔텍 중앙연구원) ;
  • 이기훈 ((주)비엔텍 중앙연구원) ;
  • 김하림 (원광대학교 한의과대학 한방생리학교실) ;
  • 권강범 (원광대학교 한의과대학 한방생리학교실) ;
  • 김선오 ((주)비엔텍 중앙연구원) ;
  • 이정한 (원광대학교 한의과대학 한방재활의학교실)
  • Ha, Ki Chan (Healthcare Claims & Management Inc.) ;
  • Kim, Hye Mi (Healthcare Claims & Management Inc.) ;
  • Baek, Hyang Im (Healthcare Claims & Management Inc.) ;
  • Jeong, Da Young (Healthcare Claims & Management Inc.) ;
  • Kim, Eun (Central R&D Center, B&Tech Co., Ltd.) ;
  • Lee, Ki Hoon (Central R&D Center, B&Tech Co., Ltd.) ;
  • Kim, Ha Rim (Department of Korean Medicinal Physiology, College of Korean Medicine, Wonkwang University) ;
  • Kwon, Kang Beom (Department of Korean Medicinal Physiology, College of Korean Medicine, Wonkwang University) ;
  • Kim, Sunoh (Central R&D Center, B&Tech Co., Ltd.) ;
  • Lee, Jung Han (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University)
  • 투고 : 2021.09.06
  • 심사 : 2021.09.29
  • 발행 : 2021.10.31

초록

Objectives Previously, we demonstrated that a 5% ethanol extract of unripe Rubus coreanus (5-uRCK) and ellagic acid has hypocholesterolemic and antiobesity activity in high-fat diet-fed animals. Therefore, we conducted a clinical study on the anti-obesity effect of 5-uRCK in 140 Korean adults (aged 19-70 years, body mass index (BMI) ≥25 kg/m2). Methods The participants were randomly assigned to two groups and were administered the placebo (n=70) or 5-uRCK extract (800 mg, n=70) daily for 12 weeks. The subjects were instructed to maintain their usual dietary intake and normal physical activity. Anthropometrics, body composition (dual-energy X-ray absorptiometry), and blood parameters were compared between the two groups. Results Average body weight and BMI did not show any significant changes; however, changes in upper body fat mass between the two groups was significantly different, as determined using analysis of covariance adjusted for baseline of percent body fat and drinking units. Moreover, Although not significant, the trunk fat mass and percent trunk fat tended to decrease after 5-uRCK supplementation. There was no significant difference in other laboratory biomarkers between the two groups. All safety parameters were within normal ranges in the both group. Conclusions In the present study, 5-uRCK significantly reduced upper body fat in obese subjects after 12 weeks, which indicates that it may help improve android-type obesity. Furthermore, the supplement is safe and well tolerated.

키워드

과제정보

본 연구는 과학기술정보통신부가 지원하고 연구개발특구진흥재단이 관리하는 지역의 미래를 여는 과학기술 프로젝트(2021-DD-UP0380)의 연구로 수행하였음.

참고문헌

  1. Bouchard C, Bray GA, Hubbard VS. Basic and clinical aspects of regional fat distribution. Am J Clin Nutr. 1990;52:946-50. https://doi.org/10.1093/ajcn/52.5.946
  2. Prineas RJ, Folsom AR, Kaye SA. Central adiposity and increased risk of coronary artery disease mortality in older women. Ann Epidemiol. 1993;3:35-41. https://doi.org/10.1016/1047-2797(93)90007-Q
  3. Hennes MM, O'Shaughnessy IM, Kelly TM, LaBelle P, Egan BM, Kissebah AH. Insulin-resistant lipolysis in abdominally obese hypertensive individuals: role of the renin-angiotensin system. Hypertension. 1996;28:120-6. https://doi.org/10.1161/01.hyp.28.1.120
  4. Ascaso JF, Romero P, Real JT, Lorente RI, Martinez-Valls J, Carmena R. Abdominal obesity, insulin resistance, and metabolic syndrome in a southern European population. Eur J Intern Med. 2003;14:101-6. https://doi.org/10.1016/S0953-6205(03)00022-0
  5. Bjorntorp P. "Portal" adipose tissue as a generator of risk factors for cardiovascular disease and diabetes. Artherosclerosis. 1990;10:493-6. https://doi.org/10.1161/01.ATV.10.4.493
  6. Bhat SP, Sharma A. Current drug targets in obesity pharmacotherapy - a review. Current Drug Targets. 2017;18(8): 983-93. https://doi.org/10.2174/1389450118666170227153940
  7. Ministry of Food and Drug Safety, National Food Safety Information Service. 2020 Health functional food production performance. Osong:Ministry of Food and Drug Safety, National Food Safety Information Service. 2020: 567-630.
  8. Ahmad M, Masood S, Sultana S, Hadda TB, Bader A, Zafar M. Report: antioxidant and nutraceutical value of wild medicinal Rubus berries. Pak J Pharm Sci. 2015;28: 241-7.
  9. Moon GS. Constituents and uses of medicinal herbs. Ilweolseogak Seoul Korea. 1991:310-1.
  10. Cho YJ, Chun SS, Kwon HJ, Kim JH, Yoon SJ, Lee KH, Comparison of physiological activities between hot-water and ethanol extracts of Bokbunja (Rubus coreanum F.). J Korean Soc Food Sci Nutr. 2005;34(6):790-6. https://doi.org/10.3746/JKFN.2005.34.6.790
  11. Yang JW, Choi IS. Comparison of the phenolic composition and antioxidant activity of Korean black raspberry, Bokbunja, (Rubus coreanus Miquel) with those of six other berries. CyTA J Food. 2017;15:110-7.
  12. Bhandary B, Lee GH, Marahatta A, Lee HY, Kim SY, So BO, Kwon JW, Song JY, Lee HK, Kim HR, Chae SW, Chae HJ. Water extracts of immature Rubus coreanus regulate lipid metabolism in liver cells. Biol Pharm Bull. 2012;35(11):1907-13. https://doi.org/10.1248/bpb.b12-00022
  13. Choung MG, Lim JD. Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J Med Crop Sci. 2012;20:259-69. https://doi.org/10.7783/KJMCS.2012.20.4.259
  14. Lee KH, Kim S. Comparison of ellagic acid contents in Korean and Chinese cultivated species of unripe black raspberries. Korean J Food Preserv. 2018;25:549-56. https://doi.org/10.11002/kjfp.2018.25.5.549
  15. Park S, Lee KH, Kang WS, Kim JS, Kim S. Endothelium-dependent and endothelium-independent vasorelaxant effects of unripe Rubus coreanus Miq. and Dendropanax morbiferus H. Lev. extracts on rat aortic rings. BMC Complement Med Ther. 2020;20(1):190. https://doi.org/10.1186/s12906-020-02977-6
  16. Shen L, Gwak SR, Joo JC, Ha KC, Park YK, Kim S, Park SJ. Effectiveness and safety of hwangchil-unripe Bokbunja extract mixture on blood pressure: a randomized double-blind placebo-controlled clinical trial. J Med Food. 2021;24(3):258-66. https://doi.org/10.1089/jmf.2020.4820
  17. Jung MA, Cho SH, Lee SY, Kim JH, Kim YS, Oh K, Yoo GS, Lee DW, Kim S. Anti-obesity effects on unripe Rubus coreanus Miquel extract in high fat diet-induced obese mice. IJBcRR. 2015;5:20-6. https://doi.org/10.9734/IJBCRR/2015/12680
  18. Oh DR, Kim Y, Choi EJ, Lee H, Jung MA, Bae D, Jo A, Kim YR, Kim S. Antiobesity effects of unripe Rubus coreanus Miquel and its constituents: an in vitro and in vivo characterization of the underlying mechanism. Evid Based Complement Alternat Med. 2016;2016:4357656.
  19. Kim KJ, Jeong ES, Lee KH, Na JR, Park S, Kim JS, Na CS, Kim YR, Kim S. Unripe Rubus coreanus miquel extract containing ellagic acid promotes lipolysis and thermogenesis in vitro and in vivo. Molecules. 2020; 25(24):5954. https://doi.org/10.3390/molecules25245954
  20. Jung MA, Cho SH, Lee SY, Kim JH, Oh K, Kim YS, Yoo GS, Lee DW, Kim S. Effects of unripe Rubus coreanus Miquel extract on improvement of lipid metabolism in C57BL/6 mice fed a high-cholesterol diet. J Korean Soc Food Sci Nutr. 2014;43:650-5. https://doi.org/10.3746/JKFN.2014.43.5.650
  21. Lee KH, Jeong ES, Jang G, Na JR, Park S, Kang WS, Kim E, Choi H, Kim JS, Kim S. Unripe Rubus coreanus Miquel extract containing ellagic acid regulates AMPK, SREBP-2, HMGCR, and INSIG-1 signaling and cholesterol metabolism in vitro and in vivo. Nutrients. 2020; 12(3):610. https://doi.org/10.3390/nu12030610
  22. Cho JM, Chae J, Jeong SR, Moon MJ, Ha KC, Kim S, Lee JH. The cholesterol-lowering effect of unripe Rubus coreanus is associated with decreased oxidized LDL and apolipoprotein B levels in subjects with borderline-high cholesterol levels: a randomized controlled trial. Lipids Health Dis. 2020;19(1):166. https://doi.org/10.1186/s12944-020-01338-z
  23. Lee JE, Park EK, Lee JE, Auh JH, Choi HK, Lee JH, Cho SM, Kim JH. Effects of a Rubus coreanus Miquel supplement on plasma antioxidant capacity in healthy Korean men. Nutr Res Pract. 2011;5:429-34. https://doi.org/10.4162/nrp.2011.5.5.429
  24. Cha HS, Park MS, Park KM. Physiological activities of Rubus coreanus Miquel. Korean J Food Sci Technol. 2001;33:409-15.
  25. Lee SM, You YH, Yoon HG, Kim KM, Park JJ, Kim SO, Ho JN, Lee JM, Shim SI, Jun WJ. Fatigue-alleviating effect on mice of an ethanolic extract from Rubus coreanus. Biosci Biotechnol Biochem. 2011;75(2):349-51. https://doi.org/10.1271/bbb.100592
  26. Choi HR, Lee SJ, Lee JH, Kwon JW, Lee HK, Jeong JT, Lee TB. Cholesterol-lowering effects of unripe black raspberry water extract. J Korean Soc Food Sci Nutr. 2013;42:1899-907. https://doi.org/10.3746/JKFN.2013.42.12.1899
  27. Woo MS, Choi HS, Seo MJ, Jeon HJ, Lee BY. Ellagic acid suppresses lipid accumulation by suppressing early adipogenic events and cell cycle arrest. Phytother Res. 2015;29(3):398-406. https://doi.org/10.1002/ptr.5264
  28. Svendsen OL, Haarbo J, Hassager C, Christiansen C. Accuracy of measurements of body composition by dual-energy x-ray absorptiometry in vivo. Am J Clin Nutr 1993;57(5):605-8. https://doi.org/10.1093/ajcn/57.5.605
  29. Jebb SA. Measurement of soft tissue composition by dual energy X-ray absorptiometry. Br J Nutr. 1997;77(2): 151-63. https://doi.org/10.1079/BJN19970021
  30. Nakamura T, Tokunaga K, Shimomura I, Nishida M, Yoshida S, Kotani K, Islam AH, Keno Y, Kobatake T, Nagai Y, Fujioka S, Tarui S, Matsuzawa Y. Contribution of visceral fat accumulation to the development of coronary artery disease in non-obese men. Atherosclerosis. 1994;107(2):239-46. https://doi.org/10.1016/0021-9150(94)90025-6
  31. Vanhala MJ, Kumpusalo EA, Pitkajarvi TK, Takala JK. Metabolic syndrome' in a middle-aged Finnish population. J Cardiovasc Risk. 1997;4(4):291-5. https://doi.org/10.1097/00043798-199708000-00010
  32. Rexrode KM, Carey VJ, Hennekens CH, Walters EE, Colditz GA, Stampfer MJ, Willett WC, Manson JE. Abdominal adiposity and coronary heart disease in women. JAMA. 1998;280(21):1843-8. https://doi.org/10.1001/jama.280.21.1843
  33. Karpe F, Pinnick KE. Biology of upper-body and lower-body adipose tissue--link to whole-body phenotypes. Nat Rev Endocrinol. 2015;11(2):90-100. https://doi.org/10.1038/nrendo.2014.185
  34. Shin SM. Management of overweight and obesity: review of the "2013 AHA/ACC/TOS guideline for the management of over-weight and obesity in adults". Korean J Med. 2014;82(2):136-41. https://doi.org/10.3904/kjm.2014.87.2.136