Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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A novel technique for recombinant protein expression in duckweed (Spirodela polyrhiza) turions

  • Chanroj, Salil (Department of Biotechnology, Faculty of Sciences, Burapha University) ;
  • Jaiprasert, Aornpilin (Department of Biotechnology, Faculty of Sciences, Burapha University) ;
  • Issaro, Nipatha (Division of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Burapha University)
  • Received : 2021.07.13
  • Accepted : 2021.08.17
  • Published : 2021.09.30
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Abstract

Spirodela polyrhiza, from the Lemnaceae family, are small aquatic plants that offer an alternative plant-based system for the expression of recombinant proteins. However, no turion transformation protocol has been established in this species. In this study, we exploited a pB7YWG2 vector harboring the eYFP gene that encodes enhanced yellow fluorescent protein (eYFP), which has been extensively used as a reporter and marker to visualize recombinant protein localization in plants. We adopted Agrobacterium tumefaciens-mediated turion transformation via vacuum infiltration to deliver the eYFP gene to turions, special vegetative forms produced by duckweeds to endure harsh conditions. Transgenic turions regenerated several duckweed fronds that exhibited yellow fluorescent emissions under a fluorescence microscope. Western blotting verified the expression of the eYFP protein. To the best of our knowledge, this is the first report of an efficient protocol for generating transgenic S. polyrhiza expressing eYFP via Agrobacterium tumefaciens-mediated turion transformation. The ability of turions to withstand harsh conditions increases the portability and versatility of transgenic duckweeds, favoring their use in the further development of therapeutic compounds in plants.

Keywords

Acknowledgement

This work was financially supported by the Research Grant of Burapha University through National Research Council of Thailand [Grant no. 30/2558].

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