Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Correlation between in vitro fertilization and artificial insemination in Holstein bulls

  • Sun, Wei (College of Life Science, Inner Mongolia University) ;
  • Li, Yunxia (College of Life Science, Inner Mongolia University) ;
  • Su, Jie (Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal) ;
  • Bao, Xiangnan (Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal) ;
  • Ding, Rui (Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal) ;
  • Zhao, Gaoping (Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal) ;
  • Cao, Guifang (Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal) ;
  • Hu, Shuxiang (Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal) ;
  • Wang, Jianguo (College of Life Science, Inner Mongolia University) ;
  • Sun, Qingyuan (Institute of Zoology Chinese Academy of Science) ;
  • Yu, Haiquan (College of Life Science, Inner Mongolia University) ;
  • Li, Xihe (College of Life Science, Inner Mongolia University)
  • Received : 2020.09.21
  • Accepted : 2021.02.16
  • Published : 2021.12.01
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Abstract

Objective: Owing to the lack of a breeding index for efficient and quick fertility evaluations of Holstein bulls when using traditional or genome-wide detection methods, this study aimed to determine whether in vitro fertilization (IVF) could be used as an indicator of conception rate of artificial insemination (AI). Methods: Conventional and sexed frozen semen from nine bulls were used for IVF and AI. Results: The IVF and AI conception rates of each bull were confirmed to be positively correlated between the conventional frozen and sexed frozen semen. The correlation coefficient R values of nine bulls between IVF and AI methods were 0.73 and 0.97 for the conventional frozen and sexed frozen semen, respectively. The average conception rate of three bulls undergoing AI was 69.5% and 64.2%, 61.8% and 58.8%, and 48.2% and 46.2% in first-, second-, and third-born cows when conventional frozen and sexed frozen semen were used, respectively, which showed a positive correlation with the fertilization rate in the same parity. We propose an evaluation standard to assess the fertilization ability of bulls based on their IVF test results, which is categorized into three grades: grade one, normal fertility bull with an AI conception rate of 40%±5% and IVF rate of 45% to 60%; grade two, higher fertility bull with an AI conception rate of 50%±5% and IVF rate of 61% to 80%; and grade three, highest fertility bull with an AI conception rate of 60%±5% and IVF rate of >80%. Conclusion: These findings reveal that IVF results can be used as a breeding index for bulls to evaluate their AI conception ability, which may shorten the time required to select bulls for breeding.

Keywords

Acknowledgement

This study was financially supported by the Project of Development and application of new technology for aerospace biological breeding of Holstein cattle, beef cattle and mutton sheep-development and application of the new technology of sex-controlled propagation (NO.ZDZX2016019), which founding from Inner Mongolia Government. The authors thank Inner Mongolia Saikexing Reproductive Biotechnology (Group) Co., Ltd.

References

  1. Kocks JEM, Broekhuijse MLWJ. The practical use of density of whole bull semen to determine the volume of an ejaculate at AI laboratories. Anim Reprod Sci 2014;146:98-102. https://doi.org/10.1016/j.anireprosci.2014.02.015
  2. Attia S, Katila T, Andersson M. The effect of sperm morphology and sire fertility on calving rate of Finnish ayrshire AI bulls. Reprod Domest Anim 2016;51:54-8. https://doi.org/10.1111/rda.12645
  3. Lecewicz M, Kordan W, Majewska A, Kaminski S, Dziekonska A, Mietelska K. Effects of the platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen (AI) with reduced sperm motility. Pol J Vet Sci 2016;19:147-58. https://doi.org/10.1515/pjvs-2016-0019
  4. Paakala E, Martin-Collado D, Maki-Tanila A, Juga J. Variation in the actual preferences for AI bull traits among Finnish dairy herds. J Anim Breed Genet 2018;135:410-9. https://doi.org/10.1111/jbg.12359
  5. Maicas C, Hutchinson IA, Kenneally J, et al. Fertility of fresh and frozen sex-sorted semen in dairy cows and heifers in seasonal-calving pasture-based herds. J Dairy Sci 2019;102:10530-42. https://doi.org/10.3168/jds.2019-16740
  6. Zhang J, Guo HM, Su J, et al. A comparison of the effects of pentoxifylline on quality of fresh and frozen-thawed bull spermatozoa. Agric Biotechnol 2014;3:20-4. https://doi.org/10.19759/j.cnki.2164-4993.2014.03.007
  7. Lopes G, Ferreira P, Rocha A. Bull frozen semen accidentally exposed to room temperature for 5 s presents reduced blastocyst formation after IVF. Anim Reprod Sci 2016;169:133. https://doi.org/10.1016/j.anireprosci.2016.03.087
  8. Kipper BH, Trevizan JT, Carreira JT, et al. Sperm morphometry and chromatin condensation in Nelore bulls of different ages and their effects on IVF. Theriogenology 2017;87:154-60. https://doi.org/10.1016/j.theriogenology.2016.08.017
  9. Holden SA, Fernandez-Fuertes B, Murphy C, et al. Relationship between in vitro sperm functional assessments, seminal plasma composition, and field fertility after AI with either non-sorted or sex-sorted bull semen. Theriogenology 2017;87:221-8. https://doi.org/10.1016/j.theriogenology.2016.08.024
  10. Seidel GE. Sexing mammalian sperm-intertwining of commerce, technology, and biology. Anim Reprod Sci 2003;79:145-56. https://doi.org/10.1016/S0378-4320(03)00162-3
  11. Moore SG, Hasler JF. A 100-Year Review: Reproductive technologies in dairy science. J Dairy Sci 2017;100:10314-31. https://doi.org/10.3168/jds.2017-13138
  12. Bousquet D, Twagiramungu H, Morin N, Brisson C, Carboneau G, Durocher J. In vitro embryo production in the cow: an effective alternative to the conventional embryo production approach. Theriogenology 1999;51:59-70. https://doi.org/10.1016/S0093-691X(98)00231-3
  13. Xu J, Guo Z, Su L, et al. Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm. J Dairy Sci 2006;89:2510-8. https://doi.org/10.3168/jds.S0022-0302(06)72326-8
  14. Marquant-Le Guienne B, Humblot P, Thibier M, Thibault C. Evaluation of bull semen fertility by homologous in vitro fertilization tests. Reprod Nutr Dev 1990;30:259-66. https://doi.org/10.1051/rnd:19900212
  15. Hunter RHF, Li XH. Egg-embryo transfer: an analytical tool for vintage experiments in domestic farm animals. J Agric Sci Technol-Iran 2013;15:65-70. https://doi.org/10.3969/j.issn.1008-0864.2013.01.11
  16. Eckert J, Niemann H. In vitro maturation, fertilization and culture to blastocysts of bovine oocytes in protein-free media. Theriogenology 1995;43:1211-25. https://doi.org/10.1016/0093-691X(95)00093-N
  17. Li XH, Iwasaki S, Nakahara T. Effects of trophectoderm dissection on development of inner cell mass of fresh and frozenthawed bovine blastocysts derived from in-vitro fertilization. J Reprod Dev 1993;39:281-6. https://doi.org/10.1262/jrd.39.281
  18. Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA. Normal development following in vitro fertilization in the cow. Biol Reprod 1982;27:147-58. https://doi.org/10.1095/biolreprod27.1.147
  19. Kim IH, Soom AV, Langendonckt AV, Bevers MM. The effect of glutathione added to IVF medium on embryo development is bull dependent. Theriogenology 1999;51:321. https://doi.org/10.1016/S0093-691X(99)91880-0
  20. McEvoy TG, Sreenan JM. Effect of embryo quality and stage of development on the survival of zona pellucida-free cattle demi-embryos. Theriogenology 1990;33:1245-53. https://doi.org/10.1016/0093-691X(90)90042-R
  21. Herr CM, Reed KC. Micronanipulation of bovine embryos for sex determination. Theriogenology 1991;35:45-54. https://doi.org/10.1016/0093-691X(91)90147-6
  22. Zhang J, Su J, Hu SX, et al. Correlation between ubiquitination and defects of bull spermatozoa and removal of defective spermatozoa using anti-ubiquitin antibody-coated magnetized beads. Anim Reprod Sci 2018;192:44-52. https://doi.org/10.1016/j.anireprosci.2018.01.018
  23. Zhang BR, Larsson B, Lundeheim N, Rodriguez-Martinez H. Relationship between embryo development in vitro and 56-day nonreturn rates of cows inseminated with frozenthawed semen from dairy bulls. Theriogenology 1997;48:221-31. https://doi.org/10.1016/S0093-691X(97)84069-1
  24. Dhali A, Anchamparuthy VM, Butler SP, Pearson RE, Gwazdauskas FC. In vitro development of bovine embryos cultured with stem cell factor or insulin-like growth factor-I following IVF with semen of two bulls having different field fertility. Anim Reprod Sci 2009;116:188-95. https://doi.org/10.1016/j.anireprosci.2009.02.007