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http://dx.doi.org/10.4489/KJM.20210038

Development of New Strains of Wolfiporia cocos for Sclerotium Formation by 2-Way Cross-Breeding  

Ka, Kang-Hyeon (Department of Forest Bioresources, National Institute of Forest Science)
Kim, Suyeon (Department of Forest Bioresources, National Institute of Forest Science)
Park, Mi-Jeong (Department of Forest Bioresources, National Institute of Forest Science)
Jeong, Yeun Sug (Department of Forest Bioresources, National Institute of Forest Science)
Ryoo, Rhim (Department of Forest Bioresources, National Institute of Forest Science)
Jang, Yeongseon (Department of Forest Bioresources, National Institute of Forest Science)
Choi, Jong-Woon (Gangwon Forest Research Institute)
Kim, Seong Hwan (College of Science & Technology, Dankook University)
Publication Information
The Korean Journal of Mycology / v.49, no.3, 2021 , pp. 405-412 More about this Journal
Abstract
Wolfiporia cocos is an important medicinal fungus that has been used in regions of Northeast Asia including Korea, Japan, and China. W. cocos is classified in Korea into two types (red bokryeong and white bokryeong) based on the internal colors (yellow orange-pale pink and white) of the sclerotium. Generally, the W. cocos type cultivated on farms produces white sclerotium. In this study, we endeavored to select strains that form sclerotium in sawdust medium using 2-way cross-breeding among two cultivated strains and three wild strains. Monospores were isolated from the fruiting bodies of cultivated and wild strains on potato dextrose agar. Thirty-nine strains of 338 hybrid strains isolated formed sclerotia with white or yellow colors upon culture for 3 months in Pinus densiflora sawdust medium. Selection for sclerotium forming strains using sawdust culture follows a very simple and easy procedure that is presented for the first time in this paper. We plan to test selected strains in the field to aid in developing new varieties for the future.
Keywords
Breeding strain; Sawdust cultivation; Sclerotium formation; Wolfiporia cocos;
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