Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Anti-inflammatory activity of indigenous Tuber himalayense in Korea

자생 Tuber himalayense 자실체 추출물의 항염증 활성

  • Kim, Minkyeong (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Hong, Hyehyun (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kim, Jung-Hwan (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kim, Seung-Young (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kim, Changmu (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources)
  • 김민경 (국립생물자원관 미생물자원과) ;
  • 홍혜현 (선문대학교 제약생명공학과) ;
  • 김정환 (선문대학교 제약생명공학과) ;
  • 김승영 (선문대학교 제약생명공학과) ;
  • 김창무 (국립생물자원관 유용자원분석과)
  • Received : 2021.08.27
  • Accepted : 2021.09.06
  • Published : 2021.09.30
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Abstract

In this study, the anti-inflammatory activity of an extract of the fruiting body of the Tuber himalayense (TH) truffle collected from oak growing areas in Korea was investigated. The extract was not cytotoxic at concentrations below 100 ㎍/mL in an experiment evaluating inflammation inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production was inhibited by the extract in a concentration-dependent manner. Western blot assay results indicated that the anti-inflammatory activity of TH extract was likely caused by the reduced production of NO and PGE2 via suppression of induced NO synthase and cyclooxygenase-2 gene expression. In addition, TH extract effectively inhibited the production of interleukin (IL)-1β and IL-6 by macrophages. Thus, TH extract effectively inhibits the overexpression of various inflammatory mediators and could be valuable in formulating anti-inflammatory foods and medicines that target these components.

본 연구는 국내 참나무 생육지에서 채집된 송로버섯의 일종인 T. himalayense 자실체 추출물(TH)의 항염증 활성을 확인하였다. LPS로 유도된 RAW 264.7 대식세포를 대상으로 한 염증 억제 실험에서 TH는 100 ㎍/ml 이하의 농도에서 세포독성을 보이지 않았으며 LPS에 의해 증가된 NO와 PGE2의 생성을 농도 의존적으로 억제하였다. Western blot 분석 결과로 볼 때, TH의 항염증 활성은 iNOS와 COX-2 유전자의 발현억제에 의해 NO와 PGE2의 생성이 감소된 것으로부터 유발된 것임을 입증한다. 또한, TH가 대식세포에 의해 생산 및 분비되는 cytokine IL-1β와 IL-6의 생성을 효과적으로 저해함을 확인하였다. T. himalayense는 다양한 염증 매개체들의 과발현을 효과적으로 억제하며, 이를 표적으로 하는 항염증 관련 식품 및 의약품에 다양하게 활용 될 수 있을 것으로 기대된다. 추후 염증 예방 물질로 적용 될 수 있는 가능성을 높이기 위하여 염증성 작용 기전에 대한 자세한 연구가 필요할 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 환경부 국립생물자원관의 지원을 받은 기관 연구과제(NIBR202102107)에 의하여 수행된 연구결과의 일부로 이에 감사드립니다.

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