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http://dx.doi.org/10.14480/JM.2021.19.3.176

Anti-inflammatory activity of indigenous Tuber himalayense in Korea  

Kim, Minkyeong (Microorganism Resources Division, National Institute of Biological Resources)
Hong, Hyehyun (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
Kim, Jung-Hwan (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
Kim, Seung-Young (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
Kim, Changmu (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources)
Publication Information
Journal of Mushroom / v.19, no.3, 2021 , pp. 176-183 More about this Journal
Abstract
In this study, the anti-inflammatory activity of an extract of the fruiting body of the Tuber himalayense (TH) truffle collected from oak growing areas in Korea was investigated. The extract was not cytotoxic at concentrations below 100 ㎍/mL in an experiment evaluating inflammation inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production was inhibited by the extract in a concentration-dependent manner. Western blot assay results indicated that the anti-inflammatory activity of TH extract was likely caused by the reduced production of NO and PGE2 via suppression of induced NO synthase and cyclooxygenase-2 gene expression. In addition, TH extract effectively inhibited the production of interleukin (IL)-1β and IL-6 by macrophages. Thus, TH extract effectively inhibits the overexpression of various inflammatory mediators and could be valuable in formulating anti-inflammatory foods and medicines that target these components.
Keywords
Anti-inflammation; Fruiting body extract; Truffle; Tuber himalayense;
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