대한본초학회지 (The Korea Journal of Herbology)

  • 제36권5호
  • /
  • Pages.101-108
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  • 2021
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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속단(續斷) 추출물의 C57BL/6 마우스를 이용한 2주 경구투여 독성시험

Two-weeks Oral Dose Toxicity Study of Dipsacus asperoides Extracts in C57BL/6 Mice

  • 허혜윤 (조선대학교 치과대학) ;
  • 신동호 (전남대학교 수의과대학) ;
  • 이지혜 (세명대학교 한의과대학) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터) ;
  • 김용범 (안전성평가연구소) ;
  • 신인식 (전남대학교 수의과대학) ;
  • 강소희 (전남대학교 수의과대학) ;
  • 손미경 (조선대학교 치과대학) ;
  • 김중선 (전남대학교 수의과대학)
  • Heo, Hye Yun (School of Dentistry, Chosun University) ;
  • Shin, Dong-Ho (College of Veterinary Medicine (BK21 Project Team), Chonnam National University) ;
  • Lee, Ji Hye (College of Korean Medicine, Semyung University) ;
  • Seo, Yun-Soo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Yong-Bum (Korea Institute of Toxicology) ;
  • Shin, Insik (College of Veterinary Medicine (BK21 Project Team), Chonnam National University) ;
  • Kang, Sohi (College of Veterinary Medicine (BK21 Project Team), Chonnam National University) ;
  • Son, Mee-Kyung (School of Dentistry, Chosun University) ;
  • Kim, Joong-Sun (College of Veterinary Medicine (BK21 Project Team), Chonnam National University)
  • 투고 : 2021.07.22
  • 심사 : 2021.09.25
  • 발행 : 2021.09.30
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초록

Objectives : A root of Dipsacus asperoides C. Y. Cheng et T. M. Ai (D. asperoides) has been traditionally used as a medicinal resource in several Asian countries, including Korean and traditional Chinese medicine that has been traditionally used for treating several medical conditions including pain, arthritis, and bone fractures in Korea. In the present study, we investigated potential subacute toxicities of D. asperoides extract. Methods : C57BL/6 mice (male, 7weeks) were randomly divided into 4 groups of 5 mice. Except for the control group, the mice were orally administrated D. asperoides extract at doses of 50, 150, or 450 mg/kg/day for 2 weeks. At the end of the treatment period, all mice were euthanized, and the following parameters were examined: mortality, body weight, clinical signs, gross findings, hematology, serum biochemistry, organ weight, and histopathology. Results : There were no abnormalities in mortality, clinical signs, body weight, gross findings, or organ weight after repeated administration of D. asperoides extract for 2 weeks, compared with the control group. In addition, there were no significant changes in hematological, serum biochemical, and histopathological parameters between the control group and D. asperoides extract administrated groups with doses of up to 450 mg/kg/day. Conclusion : In this study, D. asperoides extract showed no significant toxicities at a dose of up to 450 mg/kg/day in mice. Although we could not confirm the toxic dose of D. asperoides extract, it can be considered safe for further pharmacological use.

키워드

과제정보

본 시료를 제공한 한국한의학연구원 한약표준표본관에 감사드립니다. 본 연구는 안전성평가연구소 주요사업 연구개발비(1711133841)와 한국연구재단(2020R1A2C1004272)의 지원을 받아 수행되었습니다.

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