• Title/Summary/Keyword: RIL

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Control of histamine-forming bacteria by probiotic lactic acid bacteria isolated from fish intestine (생선 내장으로부터 분리된 프로바이오틱 유산균에 의한 히스타민 생산균의 제어)

  • Lim, Eun-Seo;Lee, Nahm-Gull
    • Korean Journal of Microbiology
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    • v.52 no.3
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    • pp.352-364
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    • 2016
  • In this study, we examined in vitro the potential probiotic properties of lactic acid bacteria (LAB) obtained from the fish intestine and their ability to degrade histamine through the production of diamine oxidase (DAO) enzymes and bacteriocin. Among 97 LAB strains isolated from the intestine of croaker, flatfish, pollack, and rockfish, CIL08, CIL16, FIL20, FIL31, PIL45, PIL49, PIL52, and RIL60 isolates exhibited excellent survival rates under simulated gastrointestinal tract conditions, high adhesion ability to HT-29 epithelial cells, and resistance to the antibiotics such as amoxicillin, ampicillin, erythromycin, penicillin G, streptomycin, tetracycline, or vancomycin. In addition, these strains did not produce histamine in decarboxylating broth containing histidine. In particular, 4 strains (CIL08, FIL20, PIL52, and RIL60) that may produce DAO were significantly able to degrade histamine. The bacteriocins produced by FIL20, FIL31, and PIL52 LAB inhibited the growth and histamine production of Enterococcus aerogenes CIH05, Serratia marcescens CIH09, Enterococcus faecalis FIH11, Pediococcus halophilus FIH15, Lactobacillus sakei PIH16, Enterococcus faecium PIH19, Leuconostoc mesenteroides RIH25, or Aeromonas hydrophilia RIH28. Histamine-producing strains isolated from fish intestine were found to reduce histamine accumulation during co-culture with CIL08, FIL20, PIL52, and RIL60 LAB showing histamine degradation or bacteriocin production ability. The probiotic strains preventing histamine formation were identified as Pediococcus pentosaceus CIL08, Lactobacillus plantarum FIL20, Lactobacillus paracasei FIL31, Lactobacillus sakei PIL52, and Leuconostoc mesenteroides RIL60 with high similarity based on 16S rRNA gene sequencing.

A Study on Smishing Block of Android Platform Environment (안드로이드 플랫폼 환경에서의 스미싱 차단에 관한 연구)

  • Lee, Si-Young;Kang, Hee-Soo;Moon, Jong-Sub
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.24 no.5
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    • pp.975-985
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    • 2014
  • As financial transactions with a smartphone has become increasing, a myriad of security threats have emerged against smartphones. Among the many types of security threats, Smishing has evolved to be more sophisticated and diverse in design. Therefore, financial institutions have recommended that users doesn't install applications with setting of "Unknown sources" in the system settings menu and install application which detects Smishing. Unfortunately, these kind of methods come with their own limitations and they have not been very effective in handling Smishing. In this paper, we propose a systematic method to detect Smishing, in which the RIL(Radio Interface Layer) collects a text message received and then, checks if message databases stores text message in order to determine whether Smishing malware has been installed on the system. If found, a system call (also known as a hook) is used to block the outgoing text message generated by the malware. This scheme was found to be effective in preventing Smishing as found in our implementation.

Genetic and Agronomic Analysis of a Recombinant Inbred Line Population to Map Quantitative Trait Loci for Blast Resistance and Select Promising Lines in Rice (벼 RIL집단의 유전 분석과 농업형질 분석을 통한 도열병 저항성 QTL 탐색 및 유망계통 선발)

  • Ha, Su-Kyung;Jeung, Ji-Ung;Jeong, Jong-Min;Kim, Jinhee;Mo, Youngjun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.3
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    • pp.172-181
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    • 2020
  • Koshihikari has been one of the most popular rice cultivars with good eating quality since the 1960s despite its susceptibility to blast disease and lodging. To map the genes controlling blast resistance and to develop promising blast-resistant breeding lines inheriting Koshihikari's high eating quality, a recombinant inbred line (RIL) population was developed from a cross between Koshihikari and a blast resistance donor with early maturity, Baegilmi. A total of 394 Koshihikari × Baegilmi RILs (KBRIL), and the two parents, were evaluated for blast resistance and major agronomic traits including heading date, culm length, panicle length, and tiller number. A linkage map encompassing 1,272.7 cM was constructed from a subset of the KBRIL (n = 142) using 130 single nucleotide polymorphisms. Two quantitative trait loci (QTL) for blast resistance, qBL1.1 harboring Pish/Pi35 and qBL2.1 harboring Pib, were mapped onto chromosomes 1 and 2, respectively. qBL1.1 was detected in both of the experimental sites, Namwon and Jeonju, while qBL2.1 was only detected in Namwon. qBL1.1 and qBL2.1 did not affect agronomic traits, including heading date, culm length, panicle length, and tiller number. From the 394 KBRILs, lines that were phenotypically similar to Koshihikari were selected according to heading date and culm length and were further divided into the following two groups based on blast resistance: Koshishikari-type blast resistant lines (KR, n = 15) and Koshishikari-type blast susceptible lines (KS, n = 15). Although no significant differences were observed in the major agronomic traits between the two groups, the KR group produced a greater mean head rice ratio than the KS group. The present study provides useful materials for developing blast-resistant cultivars that inherit both Koshihikari's high eating quality and Baegilmi's blast resistance.

Isolation of Mutants Susceptible to Rice Blast from DEB-treated Rice Population (DEB 처리에 의해 유도된 벼 돌연변이 집단으로부터 도열병 감수성 돌연변이 분리)

  • Kim, Hye-Kyung;Lee, Sang-Kyu;Han, Mu-Ho;Jeon, Yong-Hee;Lee, Gi-Hwan;Lee, Youn-Hyung;Bhoo, Seong-Hee;Hahn, Tae-Ryong;Jeon, Jong-Seong
    • Applied Biological Chemistry
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    • v.48 no.4
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    • pp.339-344
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    • 2005
  • Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.

Relationships among Viviporous Germination, Dry-heat Tolerance and Seed Longevity in Milyang 23/Gihobyeo RILs (벼 밀양 23호$\times$기호벼의 재조합 자식계통에서 수발아성,건열 저항성 및 종자수명간 상관관계)

  • Lee, Seung-Yeob;Ahn, Jeong-Ho;Kim, Hyo-Jin;Bae, Jong-Hyang;Eun, Moo-Young
    • Journal of Bio-Environment Control
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    • v.15 no.4
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    • pp.421-427
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    • 2006
  • To investigate the relationships among the traits related seed dormancy in rice, viviparous germination, dry-heat tolerance and seed longevity were tested using 164 M/G RILs ($F_{17}$) derived from the cross between Milyang 23 (Indica/Japonica) and Gihobyeo (Japonica). At 40 days after heading, the viviparous germination rates of Milyang 23 and Gihobyeo were 0.0 and 7.0%, respectively. The averaged viviparous germination rate of 164 MIG RILs was 3.2%, and their range was from 0.0 to 50.9%. In dry-heat treatment at $90^{\circ}C$ for 24 h followed by the pre-drying at $40^{\circ}C$ for 2 days, Milyang 23 showed higher germinability (98.6%,2.7 days to $T_{50}$) than Gihobyeo (37.6%, 12.9 days to $T_{50}$), and the mean germination rate of 164 M/G RILs was 72.4%. In 54 months after harvest, the germinability in Milyang 23 and Gihobyeo were 90% and 0%, and 71 lines among 164 M/G RILs showed the germinability over 90%. The relationship between seed longevity and dry-heat tolerance was linearly correlated with high positive correlation coefficient. The relationships between seed longevity and viviparous germination, and viviparous germination and dry-heat tolerance were linearly correlated with high negative correlation coefficient. Milyang 23 had higher tolerance against high dry-heat, viviparous germination and long-term storage than Gihobyeo, and 164 M/G RILs showed the medium values of parents.