Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Highly sensitive and selective enzymatic detection for hydrogen peroxide using a non-destructively assembled single-walled carbon nanotube film

탄소나노튜브 대면적 어셈블리를 통한 고감도-고선택성 과산화수소 센서 개발

  • Lee, Dongwook (Department of Fine Chemistry, Seoul National University of Science and Technology) ;
  • Ahn, Heeho (Department of Fine Chemistry, Seoul National University of Science and Technology) ;
  • Seo, Byeong-Gwuan (Department of Fine Chemistry, Seoul National University of Science and Technology) ;
  • Lee, Seung-Woo (Department of Fine Chemistry, Seoul National University of Science and Technology)
  • 이동욱 (서울과학기술대학교 정밀화학과) ;
  • 안희호 (서울과학기술대학교 정밀화학과) ;
  • 서병관 (서울과학기술대학교 정밀화학과) ;
  • 이승우 (서울과학기술대학교 정밀화학과)
  • Received : 2021.06.30
  • Accepted : 2021.07.07
  • Published : 2021.07.31
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Abstract

This study presents a simple approach for the assembly of a free-standing conductive electronic nanofilm of single-walled carbon nanotubes (SWNTs) suitable for enzymatic electrochemical biosensors. A large-scale SWNT electronic film was successfully produced by the dialysis of p-Terphenyl-4,4''-dithiol (TPDT)-treated SWNTs. Furthermore, Horseradish peroxidase (HRP) was immobilized on the TPDT-SWNT electronic film, and the enzymatic detection of hydrogen peroxide (H2O2) was demonstrated without mediators. The detection of H2O2 in the negative potential range (-0.4 V vs. Ag/AgCl) was achieved by direct electron transfer of heme-based enzymes that were immobilized on the TPDT-SWNT electronic film. The SWNT-based biosensor exhibited a wide detection range of H2O2 from 10 µM to 10 mM. The HRP-doped SWNT electronic film achieved a high sensitivity of 342 ㎛A/mM·cm2 and excellent selectivity against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, and acetaminophen.

Keywords

Acknowledgement

이 연구는 서울과학기술대학교 교내연구비의 지원으로 수행되었습니다.

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