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http://dx.doi.org/10.46670/JSST.2021.30.4.229

Highly sensitive and selective enzymatic detection for hydrogen peroxide using a non-destructively assembled single-walled carbon nanotube film  

Lee, Dongwook (Department of Fine Chemistry, Seoul National University of Science and Technology)
Ahn, Heeho (Department of Fine Chemistry, Seoul National University of Science and Technology)
Seo, Byeong-Gwuan (Department of Fine Chemistry, Seoul National University of Science and Technology)
Lee, Seung-Woo (Department of Fine Chemistry, Seoul National University of Science and Technology)
Publication Information
Journal of Sensor Science and Technology / v.30, no.4, 2021 , pp. 229-235 More about this Journal
Abstract
This study presents a simple approach for the assembly of a free-standing conductive electronic nanofilm of single-walled carbon nanotubes (SWNTs) suitable for enzymatic electrochemical biosensors. A large-scale SWNT electronic film was successfully produced by the dialysis of p-Terphenyl-4,4''-dithiol (TPDT)-treated SWNTs. Furthermore, Horseradish peroxidase (HRP) was immobilized on the TPDT-SWNT electronic film, and the enzymatic detection of hydrogen peroxide (H2O2) was demonstrated without mediators. The detection of H2O2 in the negative potential range (-0.4 V vs. Ag/AgCl) was achieved by direct electron transfer of heme-based enzymes that were immobilized on the TPDT-SWNT electronic film. The SWNT-based biosensor exhibited a wide detection range of H2O2 from 10 µM to 10 mM. The HRP-doped SWNT electronic film achieved a high sensitivity of 342 ㎛A/mM·cm2 and excellent selectivity against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, and acetaminophen.
Keywords
Single-walled carbon nanotube; Hydrogen peroxide; Direct-electron-transfer; Enzymatic biosensor; Covalent assembly;
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