DOI QR코드

DOI QR Code

별망둑 the gluttonous goby Chaenogobius gulosus 후각기관의 해부, 조직학적 특성 및 동소 망둑어과 출현종들과의 비교연구

Microscopic Characteristics of the Olfactory Organ in the Gluttonous Goby Chaenogobius gulosus(Pisces, Gobiidae), Compared to Sympatric Intertidal Gobies

  • 김현태 (전북대학교 자연과학대학 생명과학과, 전북대학교 부설 생물다양성연구소) ;
  • 박종영 (전북대학교 자연과학대학 생명과학과, 전북대학교 부설 생물다양성연구소)
  • Kim, Hyun Tae (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Jeonbuk National University) ;
  • Park, Jong Young (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Jeonbuk National University)
  • 투고 : 2020.11.28
  • 심사 : 2021.01.20
  • 발행 : 2021.03.31

초록

본 연구는 우리나라 서해의 모래와 암반 조간대에 서식하는 별망둑 Chaenogobius gulosus 후각기관의 해부 및 조직학적 구조를 확인하고 그 특징을 동소종(미끈망둑 Luciogobius guttatus, 날개망둑 Favonigobius gymnauchen)들과 비교하였다. 별망둑은 튜브형 전비공, 표면과 평행하는 후비공, 세로배열의 한 개 후판, 두 개 비낭, 후상피 하부에 풍부한 림프구, 호산구, 후감각뉴런에서 후돌기 직경의 1/4의 섬모 길이의 일반적 결과를 보여 주었다. 이러한 특징들 중 1) 0.5~1.0 mm의 전비공 직경, 0.2~0.5 mm의 후비공 직경(vs. 미끈망둑의 0.2~0.3 mm, 0.2~0.3 mm; 날개망둑의 0.2~0.4 mm, 0.1~0.3 mm의 전비공과 후비공 직경), 2) 감각상피에서의 상대적으로 풍부한 림프구, 3) 비감각섬모의 부재(vs. 미끈망둑에서 높은 분포; 날개망둑에서 낮은 분포), 4) 후감각뉴런의 후돌기 대비 1/4의 섬모 길이(vs. 미끈망둑의 1/4에서 1 : 1 비율 혼재; 날개망둑의 2~3배)는 두 동소종들과 비교되는 특이적인 결과였다. 결론적으로, 별망둑 후각기관의 해부 및 조직학적 특징들은 모래와 암반 조간대에서 다른 동소종들 보다 더 적극적인 면역반응과 활동적인 움직임에 적응된 결과로 간주된다.

Using stereo, light, and scanning electron microscopes, we researched the anatomical and histological structure of Chaenogobius gulosus's olfactory organ and compared it to those of sympatric gobies Luciogobius guttatus and Favonigobius gymnauchen. Results revealed the following common characteristics: i) tubular anterior nostril (AN) and flat posterior nostril (PN), ii) a single longitudinal lamella, iii) two accessory nasal sacs (ANS, ethmoidal and lacrimal), iv) abundant sensory epithelium lymphatic cells (LC), v) an eosinophil cell, and vi) a ciliary length a quarter of the knob diameter in the olfactory receptor neuron (ORN). Some characteristics are specific to C. gulosus and different from the other two gobies: i) 0.5~1.0 mm AN and 0.2~0.5 mm PN (vs. 0.2~0.3 mm and 0.2~0.3 mm in L. guttatus; 0.2~0.4 mm and 0.1~0.3 mm in F. gymnauchen), ii) two ANS (vs. absence in L. guttatus; two in F. gymnauchen), iii) abundant LC (vs. low in L. guttatus and F. gymnauchen), iv) low density non-sensory cilia on the lamellar surface (vs. high in L. guttatus; low in F. gymnauchen), and v) a quarter ciliary length to knob diameter ratio in the ORN (vs. mixture of a quarter to equal ratio in L. guttatus; two or three times in F. gymnauchen). From these results, we confirmed the C. gulosus olfactory organ has adapted anatomically and histologically to the sand-rock tidal zone.

키워드

과제정보

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1 A6A3A0109608211).

참고문헌

  1. Baeck, G.W., C.I. Park, J.M. Jeong, M.C. Kim, S.H. Huh and J.M. Park. 2010. Feeding habits of Chaenogobius gulosus in the coastal waters of Tongyeong, Korea. Korean J. Ichthyol., 22: 41-48.
  2. Barbar, V.C. and A. Boyde. 1968. Scanning electron microscopic studies of cilia. Z. Zellforsch, 84: 269-284. https://doi.org/10.1007/BF00330870
  3. Chernova, N.V. 2008. Systematics and phylogeny of fish of the genus Liparis (Liparidae, Scorpaeniformes). J. Ichthyol., 48: 831852. https://doi.org/10.1134/S0032945208100020
  4. Cox, J.P. 2008. Hydrodynamic aspects of fish olfaction. J. R. Soc. Interface, 5: 575-593. https://doi.org/10.1098/rsif.2007.1281.
  5. Doving, K.B., M. Dubois-Dauphin, A. Holley and F. Jourdan. 1977. Functional anatomy of the olfactory organ of fish and the ciliary mechanism of water transport. Acta Zool., 58: 245-255. https://doi.org/10.1111/j.1463-6395.1977.tb00260.x.
  6. Hansen, A. and B.S. Zielinski. 2005. Diversity in the olfactory epithelium of bony fishes: development, lamellar arrangement, sensory neuron cell types and transduction components. J. Neurocytol., 34: 183-208. https://doi.org/10.1007/s11068-005-8353-1
  7. Hansen, A. and Z. Eckart. 1998. The peripheral olfactory organ of the zebrafish, Danio rerio: an ultrastructural study. Chem. Senses, 23: 39-48. https://doi.org/10.1093/chemse/23.1.39
  8. Hara, T.J. 1986. Role of olfaction in fish behaviour. In: Pitcher, T.J. (ed.), The behaviour of teleost fishes. Croom Helm, London, U.K., pp. 152-176.
  9. Hirase, S. and M. Ikeda. 2014. Divergence of mitochondrial DNA lineage of the rocky intertidal goby Chaenogobius gulosus around the Japanese Archipelago: reference to multiple Pleistocene isolation events in the Sea of Japan. Mar. Biol., 161: 565-574. https://doi.org/10.1007/s00227-013-2359-5.
  10. Horn, M.H., K.L. Martin and M.A. Chotkowski. 1999. Intertidal Fishes: Life in Two Worlds. Academic Press, San Diego, U.S.A., 399pp.
  11. Kasumyan, A.O. 2004. The olfactory system in fish: structure, function, and role in behavior. J. Ichthyol., 44: 180-223.
  12. Kim, H.T. 2018. A comparative anatomy and histology of the olfactory organ of 17 fishes in South Korea. Doctoral Thesis, Chonbuk National University, Jeonju, Korea, 175pp.
  13. Kim, H.T., H.S. Kim and J.Y. Park. 2016. The anatomy and histology of the olfactory organ in the Korean sand goby Favonigobius gymnauchen (Pisces, Gobiidae). Korean J. Ichthyol., 28: 2834.
  14. Kim, H.T. and J.Y. Park. 2016. The anatomy and histoarchitecture of the olfactory organ in the Korean flat-headed goby Luciogobius guttatus (Pisces; Gobiidae). Appl. Microsc., 46: 51-57. https://doi.org/10.9729/AM.2016.46.1.51.
  15. Kim, H.T. and J.Y. Park. 2020. Microscopic research on the olfactory organ of the Far Eastern brook lamprey Lethenteron reissneri (Pisces, Petromyzontidae). Appl. Microsc., 50: 1-7.
  16. Kim, H.T., S.W. Yun and J.Y. Park. 2019. Anatomy, histology, and histochemistry of the olfactory organ of the Korean shuttles mudskipper Periophthalmus modestus. J. Morphol., 280: 14851491. https://doi.org/10.1002/jmor.21044.
  17. Kim, H.T., Y.J. Lee, J.S. Park and J.Y. Park. 2014. A study on the structure of peripheral olfactory organ in the Korean mudskipper, Scartelaos gigas (Pisces, Gobiidae). Korean J. Ichthyol., 26: 281-287.
  18. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated Book of Korean Fishes. Kyohak Publishing Co. Ltd., Seoul, Korea, 615pp.
  19. Kim, S.Y., C.B. Park, J.W. Kang, Y.C. Choi, S. Rho, H.J. Bawk, H.B. Kim and Y.D. Lee. 2004. Gonadal development and reproductive cycle of gluttonous goby Chasmichthys gulosus (Guichenot). Korean J. Ichthyol., 16: 261-270.
  20. Menco, B. 1992. Ultrastructural studies on membrane, cytoskeletal, mucous, and protective compartments in olfaction. Microsc. Res. Tech., 22: 215-224. https://doi.org/10.1002/jemt.1070220303.
  21. Masselink, G. and R. Gehrels. 2014. Coastal environments and global change. John Wiley and Sons, Chichester, U.K., 438pp.
  22. Oh, J., T.W. Kim and S. Kim. 2016. The complete mitochondrial genome of Chaenogobius gulosus (Gobiidae, Perciformes) from the South Sea, Korea. Mitochondrial DNA A. DNA Mapp. Seq. Anal., 27: 4207-4208. https://doi.org/10.3109/19401736.2015.1022742.
  23. Press, C.M. and O. Evensen. 1999. The morphology of the immune system in teleost fishes. Fish Shellfish Immunol., 9: 309-318. https://doi.org/10.1006/fsim.1998.0181
  24. Thornhill, R.A. 1967. The ultrastructure of the olfactory epithelium of the lamprey Lampetra fluviatilis. J. Cell Sci., 2: 591-602. https://doi.org/10.1242/jcs.2.4.591