DOI QR코드

DOI QR Code

형광과 레이저 스펙클 대조도 이미징을 결합한 실시간 의료영상 시스템 개발

Development of a Real-time Medical Imaging System Combined with Laser Speckle Contrast Imaging and Fluorescence Imaging

  • Shim, Min Jae (Department of Biomedical Engineering, Pukyong National University) ;
  • Kim, Yikeun (Department of Research and Development) ;
  • Ko, Taek Yong (Kosin University Gospel Hospital) ;
  • Choi, Jin Hyuk (Kosin University Gospel Hospital) ;
  • Ahn, Yeh-Chan (Department of Biomedical Engineering, Pukyong National University)
  • 투고 : 2021.06.17
  • 심사 : 2021.06.23
  • 발행 : 2021.06.30

초록

It is important to differentiate between the target tissue (or organ) and the rest of the tissue before incision during surgery. And when it is necessary to preserve the differentiated tissues, the blood vessels connected to the tissue must be preserved together. Various non-invasive medical imaging methods have been developed for this purpose. We aimed to develop a medical imaging system that can simultaneously apply fluorescence imaging using indocyanine green (ICG) and laser speckle contrast imaging (LSCI) using laser speckle patterns. We designed to collect images directed to the two cameras on a co-axial optical path and to compensate equal optical path length for two optical designs. The light source used for fluorescence and LSCI the same 785 nm wavelength. This system outputs real-time images and is designed to intuitively distinguish target tissues or blood vessels. This system outputs LSCI images up to 37 fps through parallel processing. Fluorescence for ICG and blood flow in animal models were observed throughout the experiment.

키워드

과제정보

이 논문은 부경대학교 자율창의학술연구비(2019년)에 의하여 연구되었음.

참고문헌

  1. Draijer M, Hondebrink E, van Leeuwen T, Steenbergen W. "Review of laser speckle contrast techniques for visualizing tissue perfusion." Lasers in Medical Science, 2009;24(4):639-651. https://doi.org/10.1007/s10103-008-0626-3
  2. Briers JD, Webster S. "Laser speckle contrast analysis (LASCA): a nonscanning, full-field technique for monitoring capillary blood flow." Journal of Biomedical Optics, 1996;1(2):174-179. https://doi.org/10.1117/12.231359
  3. Fercher AF, Briers JD. "Flow visualization by means of single-exposure speckle photography." Optics Communications, 1981;37(5):326-330. https://doi.org/10.1016/0030-4018(81)90428-4
  4. Briers JD. "Laser Doppler and time-varying speckle: a reconciliation." JOSA A, 1996;13(2):345-350. https://doi.org/10.1364/JOSAA.13.000345
  5. Rege A, Murari K, Seifert A, Thakor NV, Pathak AP. "Multiexposure laser speckle contrast imaging of the angiogenic microenvironment." Journal of Biomedical Optics, 2011;16(5):056006. https://doi.org/10.1117/1.3582334
  6. Alander JT, Kaartinen I, Laakso A, Patila T, Spillmann T, Tuchin VV, Venermo M, Valisuo P. "A review of indocyanine green fluorescent imaging in surgery." International Journal of Biomedical Imaging, 2012;2012:26.
  7. Frangioni JV. "In vivo near-infrared fluorescence imaging." Current Opinion in Chemical Biology, 2003;7(5):626-634. https://doi.org/10.1016/j.cbpa.2003.08.007
  8. Nguyen JM, Hogen L, Laframboise S, Bouchard-Fortier G, Ferguson SE, Bernardini MQ, May T. "The use of indocyanine green fluorescence angiography to assess anastomotic perfusion following bowel resection in surgery for gynecologic malignancies-A report of 100 consecutive anastomoses." Gynecologic Oncology, 2020;158(2): 402-406. https://doi.org/10.1016/j.ygyno.2020.05.008
  9. Griffiths M, Chae MP, and Rozen WM. "Indocyanine greenbased fluorescent angiography in breast reconstruction." Gland Surgery, 2016;5(2):133.
  10. Hultman M, Fredriksson I, Larsson M, Alvandpour A, Stromber, T. "A 15.6 frames per second 1-megapixel multiple exposure laser speckle contrast imaging setup." Journal of Biophotonics, 2018;11(2): e201700069. https://doi.org/10.1002/jbio.201700069
  11. Zolei-Szenasi D, Czimmer S, Smausz T, Domoki F, Hopp B, Kemeny L, Bari F, Ivanyi I. "Enhancements on multi-exposure LASCA to reveal information of speed distribution." Journal of the European Optical Society-Rapid publications, 2015;10:15033. https://doi.org/10.2971/jeos.2015.15033
  12. Yang O, Cuccia DJ, Choi B. "Real-time blood flow visualization using the graphics processing unit." Journal of Biomedical Optics, 2011;16(1):016009. https://doi.org/10.1117/1.3528610
  13. Wang Y, Lv W, Chen X, Lu J, Li P. "Improving the sensitivity of velocity measurements in laser speckle contrast imaging using a noise correction method." Optics Letters, 2017;42(22):4655-4658. https://doi.org/10.1364/OL.42.004655