Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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The Effects of Gobonyangjeonbang Administration on Reproductive Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced Rats.

고본양정방 투여가 TCDD 유도 랫드의 생식독성에 미치는 영향

  • OH, Ji Hye (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Yang, Dong Hyun (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Park, Un kyu (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Cho, Chung Sik (Department of Korean Medicine, Daejeon University) ;
  • Hwang, Seock Yeon (Department of Biomedical Laboratory Science, Daejeon University)
  • 오지혜 (대전대학교 임상병리학과) ;
  • 양동현 (대전대학교 임상병리학과) ;
  • 박운규 (대전대학교 임상병리학과) ;
  • 조충식 (대전대학교 한의과대학) ;
  • 황석연 (대전대학교 임상병리학과)
  • Received : 2021.01.04
  • Accepted : 2021.04.02
  • Published : 2021.04.30
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Abstract

Sperm formation disorders and sperm quality degradation comprises the largest proportion of male infertility caused by TCDD. To solve this problem, this study examined the effects of Gobonyangjeonbang oriental medicine prescription on the endocrine function and reproductive toxicity-related indicators in rat-induced TCDD-induced reproductive. Male SD rats were divided into five groups of seven animals and tested. The normal control group was administered the vehicle and saline, the TCDD alone group was administered intraperitoneally with TCDD (2 ㎍/kg, weeks) and physiological saline, and the test group was administered orally by dividing GYB (75, 150, and 300 mg/kg) into three concentrations for six weeks. Weight loss was observed in all groups administered TCDD. Regarding the hormonal changes, a significant decrease in free testosterone was observed in the GYB 300 mg/kg group (p<0.01). In addition, some of the germ cell destruction, seminiferous tubular atrophy, and decrease in sperm count was improved in a concentration-dependent manner in the testicular tissue of the GYB-treated group. In addition, Johnsen's score and serotoli cell index (SCI) were improved in a concentration-dependent manner (p<0.05). Overall, GYB can be used in drug therapy rather than medical procedures to solve male infertility in the future.

TCDD로 유발할 수 있는 남성 난임 원인 중 가장 큰 비율을 차지하는 것은 정자형성 장애와 정자의 질적 저하가 있다. 이를 해결하기 위해 GYB의 한의학적 처방을 이용하여 TCDD-유도 생식독성을 유발한 랫드에서 내분비기능 및 생식독성 관련 지표에 미치는 영향을 조사하고자 본 연구를 시행하였다. 수컷 SD 랫드를 5개의 그룹으로 7마리씩 나누어 실험하였다. 정상 대조군은 vehicle 및 saline을 투여하였으며, TCDD 단독투여그룹은 TCDD(2 ㎍/kg, weeks) 및 생리식염수를 복강 내 투여하였으며, 시험 군은 6주 동안 GYB (75, 150, 300 mg/kg)를 3가지 농도로 나누어 경구로 투여하였다. TCDD를 투여한 모든 군에서 체중 감소가 나타났으며, 호르몬 변화에서 GYB 300 mg/kg 투여군에서 free testosterone의 유의한(p<0.01) 감소가 나타났다. 또한 GYB 투여군의 고환조직에서 생식세포 일부 파괴, 정세관 위축 그리고 정자 수의 감소가 농도 의존적으로 개선되는 것이 관찰되었다. 또한 Johnsen's score, serotoli cell index(SCI)에서도 농도 의존적으로 개선되었다(p<0.05). 이러한 발견은 향후 남성 난임의 해결방안으로 의학적 시술이 아닌 약물요법을 효과적으로 사용할 수 있을 것으로 기대할 수 있다.

Keywords

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