Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Simultaneous determination of saikosaponin derivatives in Bupleurum falcatum by HPLC-ELSD analysis using different extraction methods

  • Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kang, Sam Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2021.01.05
  • Accepted : 2021.01.15
  • Published : 2021.03.31
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Abstract

Saikosaponin derivatives such as saikosaponins A, B1, B2, B3, B4, C, and D present in Bupleurum falcatum were analyzed by a high performance liquid chromatograph equipped with an evaporative light scattering detector, using different extraction solvents (water and 70% ethanol). The samples were injected into a YMC Pack Pro C18 column and separated using a gradient elution system with a mobile phase composed of acetonitrile and water at a flow rate of 1.1 mL/min. The content of saikosaponin derivatives was higher in 70% ethanol extract than in water extract. This study provides an efficient analytical method for determining the optimal conditions for extraction of saikosaponin derivatives, which can be used as a basis for development of functional foods and pharmaceutical products from B. falcatum.

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References

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