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http://dx.doi.org/10.3839/jabc.2021.009

Simultaneous determination of saikosaponin derivatives in Bupleurum falcatum by HPLC-ELSD analysis using different extraction methods  

Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University)
Kim, Juree (Department of Plant Science and Technology, Chung-Ang University)
Kang, Sam Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University)
Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.1, 2021 , pp. 57-61 More about this Journal
Abstract
Saikosaponin derivatives such as saikosaponins A, B1, B2, B3, B4, C, and D present in Bupleurum falcatum were analyzed by a high performance liquid chromatograph equipped with an evaporative light scattering detector, using different extraction solvents (water and 70% ethanol). The samples were injected into a YMC Pack Pro C18 column and separated using a gradient elution system with a mobile phase composed of acetonitrile and water at a flow rate of 1.1 mL/min. The content of saikosaponin derivatives was higher in 70% ethanol extract than in water extract. This study provides an efficient analytical method for determining the optimal conditions for extraction of saikosaponin derivatives, which can be used as a basis for development of functional foods and pharmaceutical products from B. falcatum.
Keywords
Bupleurum falcatum; Extraction method; High performance liquid chromatograph; Quantitative analysis; Saikosaponin;
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