Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibitors of Antigen-induced Degranulation of RBL-2H3 Cells Isolated from Amomum tsao-ko

초과(草果)의 RBL-2H3 세포 항원 유도 탈과립 억제성분

  • Jeong, Wonsik (Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)) ;
  • Hong, Seong Su (Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)) ;
  • Park, Sun-Mi (Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)) ;
  • Lee, Jung A (Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)) ;
  • Park, Ju-Hyoung (College of Pharmacy, Dankook University) ;
  • Ahn, Eun-Kyung (Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)) ;
  • Choi, Chun Whan (Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)) ;
  • Oh, Joa Sub (College of Pharmacy, Dankook University)
  • Received : 2020.11.04
  • Accepted : 2020.12.28
  • Published : 2021.03.31
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Abstract

Bioactivity-guided fractionation of EtOH extract of the dried fruits of Amomum tsao-ko led to isolation of three compounds (1-3). Their structures were elucidated by spectroscopic methods (MS, 1D and 2D-NMR) and comparison with literature values, as naringenin-5-O-methyl ether (1), helichrysetin (2), and cardamomin (3). Compound 2 was obtained from the genus Amomum for the first time. Among them, compounds 2 and 3 inhibited on the release of β-hexosaminidase from RBL-2H3 cells, with 99.1 and 21.3% at the concentration of 50 μM, respectively.

전통생약으로서 다양한 효능과 향신료, 조미료로서 사용되어온 초과(草果)의 항알레르기 성분연구를 위해 80% 에탄올 추출물 중 CH2Cl2, 분획으로부터 3 종의 flavonoid를 단리하였으며, 이들의 물리화학적 성상과 분광분석 데이터로부터 구조를 확인한 결과 naringenin-5-O-methyl ether (1), helichrysetin (2) 및 cardamomin (3)로 각각 동정하였다. 이들 화합물 중 화합물 2는 Amomum 속으로부터 처음 규명되었고, 화합물 3은 이 식물에서 처음 보고되는 화합물이다. 또한 화합물 2와 3은 RBL-2H3 세포로부터 β-hexosaminidase assay를 수행한 결과 탈과립 억제효과를 나타내었다. 따라서 초과의 chalcone 성분은 탈과립 억제효능을 통하여 알러지 질환에 유용할 것으로 사료된다.

Keywords

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