Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Simultaneous determination of phytochemical constituents in Paeonia lactiflora extracts using the HPLC-UV method

  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kang, Sam Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2020.12.16
  • Accepted : 2020.12.28
  • Published : 2021.03.31
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Abstract

Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A reverse-phase column was used in a gradient elution system, and UV detection was performed at 280 nm. The results showed that the quantity of paeoniflorin was the highest in ethanol and water extracts (73.89 and 57.87 mg/g, respectively) among the six compounds. This study contributes a good analysis method for the contents of P. lactiflora and would be propitious for developing medicines and functional foods.

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References

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