Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Whitening and Antioxidant Effects of Extracts from Angelica gigas Nakai Thin Root

참당귀 세미 추출물의 미백 및 항산화 효과

  • Lee, Sang-Hoon (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Jeong-Yong (Herbal Crop Utilization Research Team, National Institute of Horticultural & Herbal Science, RDA) ;
  • Seo, Kyung Hye (Floriculture Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Jang Nam (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Yoon-Jeong (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Moon, Youn-Ho (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hur, Mok (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Woo Tae (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Huh, Yun Chan (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chang, Jae-Ki (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Koo, Sung Cheol (Herbal Crop Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 이상훈 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 박정용 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) ;
  • 서경혜 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 최장남 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 이윤정 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 문윤호 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 허목 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 박우태 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 허윤찬 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 장재기 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 구성철 (농촌진흥청 국립원예특작과학원 약용작물과)
  • Received : 2020.09.07
  • Accepted : 2020.11.30
  • Published : 2021.02.01
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Abstract

Angelica gigas Nakai (AGN) is a perennial plant belonging to the family Apiaceae. Its root has been utilized as a traditional medicine especially in Korea. This study was carried out to evaluate the potential use of extracts from AGN root parts as a cosmetic material. The dried AGN roots are divided into body (B), thick root (TkR), medium root(MR) and thin root (TnR) according to their diameter before cutting into medicine. B, TkR and MR of AGN are combined and used as medicinal herbs (MH). The extracts from AGN each root part (B, TkR, MR, TnR, MH) were used to test the effect on cell viability using MTS assay and to examine inhibitory effect on melanin accumulation in B16F10 melanoma cells. All extracts (50 - 200 ㎍/mL) from the each root part did not affect the cell viability. And inhibitory effect of all root extracts (200 ㎍/mL) on melanin accumulation was 12-19%. Especially, TnR showed similar inhibitory effect on melanin accumulation to MH. In addition, DPPH and ABTS free radical scavenging activity were higher in the TnR extract compared to MH. This study showed that the TnR extract exhibit high inhibitory effect on melanin accumulation and antioxidant activity compared to MH, suggesting that TnR extract has potential as a cosmetic ingredient.

본 연구는 참당귀 가공 시에 버려지는 부산물인 세미를 기능성 화장품 원료로 활용가능성이 있는지 평가하기 위해 수행하였다. MTS assay 수행결과 70% 주정을 이용한 뿌리 부위별 추출물의 경우 처리농도 25 ~ 200 ㎍/mL까지 세포독성이 없었다. 추출물 200 ㎍/mL를 처리하였을 때의 melanin 생성 억제효과는 추출 부위별로 12% ~ 19%로 나타났으며, 세미와 약재의 melanin 생성 억제효과가 비슷하게 나타났다. 그리고 DPPH radical 소거능이 50%에 달하는 IC50 값은 세미 677.9 ± 30.5 ㎍/mL, 약재 728.0 ± 42.4 ㎍/mL로 나타났고, ABTS radical 소거능이 50%에 달하는 IC50 값은 세미 114.9 ± 0.1 ㎍/mL, 약재 173.6 ± 1.3 ㎍/mL으로 나타나 세미가 약재보다 항산화활성이 높게 나타났다. 따라서 본 실험결과를 고려해 봤을 때, 부산물인 세미는 기능성 화장품 원료로 이용 가능성이 높은 것으로 생각된다.

Keywords

References

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