DOI QR코드

DOI QR Code

Anti-inflammatory Effect of Berchemia berchemiaefolia Leaves through Inhibition of NF-κB and MAPK Signaling Activation in LPS-stimulated RAW264.7 Cells

망개나무 추출물의 NF-κB 및 MAPK 신호전달 억제를 통한 항염증 효과

  • Kim, Da Som (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Eo, Hyun Ji (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kang, Yeongyeong (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 김다솜 (국립산림과학원 산림약용자원연구소) ;
  • 어현지 (국립산림과학원 산림약용자원연구소) ;
  • 강연경 (국립산림과학원 산림약용자원연구소) ;
  • 박광훈 (국립산림과학원 산림약용자원연구소)
  • Received : 2020.09.10
  • Accepted : 2020.12.15
  • Published : 2021.02.01

Abstract

Berchemia berchemiaefolia (Makino) Koidz. is found not only in korea, but also in japan and is known as a rare plant all over the world. In this study, we evaluated anti-inflammatory effect of B. berchemiaefolia (Makino) Koidz. leaves (BBK-L) in LPS-stimulated RAW264.7 cells. BBK-L inhibited the generation of NO through the suppression of iNOS experession. BBK-L attenuated the expression of iNOS, COX-2, TNF-α, IL-1β, IL-6 induced by LPS. BBK-L decreased LPS-mediated IκB-α degradation inhibite which resulted in the inhibition of NF-κB activation in RAW264.7 cells. In addition, BBK-L suppressed ERK1/2, JNK phosphorylation induced by LPS. BBK-L showed anti-inflammatory effect through blocking the generation of the inflammatory mediators such as NO, iNOS, COX-2, TNF-α, IL-1β, IL-6 via the inhibiting of NF-κB and MAPK signaling activation. These results suggests that BBK-L may have great potential for the development of anti-inflammatory drug to treat acute and chronic inflammatory disorders.

본 연구는 망개나무 추출물의 항염증 효과를 구명하기 위하여 수행되었다. 망개나무를 이용한 다양한 기능성 연구가 진행되고 있으나 망개나무 항염증에 대한 연구는 과학적 근거가 부족한 실정이다. 따라서 망개나무 잎(BBK-L) 추출물이 마우스 대식세포인 RAW264.7 세포에서 LPS에 의한 염증반응에 미치는 영향을 평가하였다. LPS로 유도된 마우스 대식세포 RAW264.7에 BBK-L 추출물을 농도별로 처리하였을 때, NO 생성량과 만성염증 유발인자인 iNOS, COX-2, IL-1β, TNF-α및 IL-6 발현 감소를 확인하였으며, NF-κB 및 MAPK의 인산화를 억제하는 것을 확인하였다. 이러한 연구결과를 토대로 BBK-L 추출물이 만성 염증질환의 예방과 치료 또는 기능성 소재로 활용될 수 있는 기초적인 정보를 제공할 수 있을 것으로 사료된다.

Keywords

References

  1. Atreya, R. and M.F. Neurath. 2005. Involvement of IL-6 in the pathogenesis of iflammatory bowel disease and colon cancer. Clin. Rex. Allergy Immunol. 28:187-196. https://doi.org/10.1385/CRIAI:28:3:187
  2. Boumpas, D.T., G.P. Chrousos and R.L wilder. 1993. Glucocorticoid therapy for immune mediated disease: Casic and clinical correlates. Ann. Intern. Med. 119:1198-208. https://doi.org/10.7326/0003-4819-119-12-199312150-00007
  3. Chang, C.S., H.S. Lee, T.Y. Park and H. Kim. 2005. Reconsideration of rare and endangered plant species in Korea based on the IUCN red list categories. Korean J. Ecology 28:305-320. https://doi.org/10.5141/JEFB.2005.28.5.305
  4. Chi, H.J. 1998. Anticancer herb from Korean medicinal plants. Korean J. Plant Res. 2:1-17.
  5. Choi, S.C., M.H. Joo and G.H. Lee. 2015. The influence of Korean Berchemia berchemiaefolia (Makino) Koidz extract on nonalcoholic hepatic steatosis in rats. AJMAHS 5:131-142. https://doi.org/10.14257/AJMAHS.2015.06.29
  6. Coskun, M., J. Olsen, J.B. Seidelin and O.H Nielsen. 2011. MAP kinases in inflammatory bowel disease. Clin. Chim. Acta. 412(7-8):513-520. https://doi.org/10.1016/j.cca.2010.12.020
  7. Guha, M. and N. Mackman. 2001. LPS induction of gene expression in human monocytes. Cell Signal. 13(2):85-94. https://doi.org/10.1016/S0898-6568(00)00149-2
  8. Guzik, T.J., R. Korbut and T. Adamek-Guzik. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 54:469-487.
  9. Hsieh, C.Y., M.J. Hsu, G. Hsiao, Y.H. Wang, C.W. Huang, S.W. Chen, T. Jaya-kumar, P.T. Chiu, Y.H. Chiu and J.R. Sheu. 2011. Andrographolide enhances nuclear factor-kB subunit p65 Ser 536 dephosphorylation through activation of protein phosphatase 2A in cascular smooth muscle cells. J. Biol. Chem. 286:5942-5955. https://doi.org/10.1074/jbc.M110.123968
  10. Ilorton, J.K., A.S. Williams, Z. Smith-Philips, R.C. Martin and G. O'Beirne. 1999. Intracellular measurement of prostaglandin E2 : Effects of anti-inflammatory drugs on cyclooxygenase activity and prostanoid expression. Anal. Biochem. 271:18-28. https://doi.org/10.1006/abio.1999.4118
  11. Jin, B.M., G.H. Lee and K.Y. Hyun. 2014. Antidepressant-like effects of Berchemia berchemiaefolia via NF-κB signal transduction in rat models of depression. AJMAHS 4(2):79-90. https://doi.org/10.14257/AJMAHS.2014.12.20
  12. Kim, J.D., S.B. Park, H.J. Eo, G.H. Park and J.B. Jeong. 2020. Induction of apoptosis by Sageretia thea branch extracts through activation of NF-κB signaling pathway in human colorectal cancer cells. Korean J. Plant Res. 33(5):428-435. https://doi.org/10.7732/KJPR.2020.33.5.428
  13. Lee, H. J., E.A. Hyun, W.J. Yoon, B.H. Kim, M.H. Rhee, H.K. Kang, J.Y, Cho and E.S. Yoo. 2006. In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts. J. Ethnopharmacology 103:208-216. https://doi.org/10.1016/j.jep.2005.08.009
  14. Lee, J.H., C.W. Yun and S.C. Hong. 2005. Community and population structure of Berchemia berchemiaefolia Forest. Korean J. For. Soc. 94(4):269-274.
  15. Moynagh, P.N. 2005. The NF-kappa B pathway. J. Cell Sci. 118:4589-92. https://doi.org/10.1242/jcs.02579
  16. Namkoong, S., S.A. Jang, E.H. Sohn, J.P. Bak, E. Sohn, H.J. Koo, W.J. Yoon, J.E. Kwon, Y.J. Jeong, X. Meng, H.S. Han and S.C. Kang. 2015. Comparative study of Litsea japonica leaf and fruit extract on the anti-inflammatory effects. Korean J. Plant Res. 28(2):145-152. https://doi.org/10.7732/kjpr.2015.28.2.145
  17. Rabson, A., I.M. Roitt and P.J. Delves. 2005. Really Essential Medical Immunology. Blackwell Publishing Ltd., Hoboken, NJ (USA). pp. 1-14.
  18. Shin, J., S., J.M. Kim and W.G. An. 2012. Anti-inflammatory effect of red ginseng through regulation of MAPK in lipopolysaccharide-stimulated RAW264.7. J. Physiol. Pathol. Korean Med. 26:293-300.
  19. Sim, M.O., H.J. Lee, J.H. Jang, H.E. Lee, H.K. Jung, T.M. Kim, J.H No, J. Jung, D.E Jung and H.W. Cho. 2017. Anti-inflammatory and antioxidant effects of Spiraea prunifolia Sieb. Et Zucc. var. simpliciflora Nakai in RAW 264.7 cells. Korean J. Plant Res. 30:335-342. https://doi.org/10.7732/kjpr.2017.30.4.335
  20. Wang, Y., J. Xu, Y. Meng, I.M. Adcock and X. Yao. 2018. Role of inflammatory cells in airway remodeling in COPD. Int J. Chron. Obstruct. Pulmon. Dis. 13:3341-3348. https://doi.org/10.2147/copd.s176122
  21. Zhang, Y., T. Pizzute and M. Pei. 2014. A review of crosstalk between MAPK and Wnt signals and its impact on cartilage regeneration. Cell Tissue Res. 358(3):633-649. https://doi.org/10.1007/s00441-014-2010-x