Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Critical Low Temperature and Response of Behavioral Tolerance in Red Seabream Pagrus major fingerlings Exposed to Cold Shock

저온 충격에 노출된 참돔 Pagrus major 치어의 임계 저 수온 및 행동 내성 반응

  • Yoon, Sung Jin (Ulleungdo-Dokdo Ocean Science Station, Korea Institute of Science and Technology)
  • 윤성진 (한국해양과학기술원 울릉도.독도해양연구기지)
  • Received : 2020.08.31
  • Accepted : 2021.01.08
  • Published : 2021.01.31
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Abstract

The critical low temperature and response of the behavioral tolerance of red sea bream Pagrus major fingerlings were determined using the continuous behavior monitoring system (CBMS). The behavior of the experimental organisms was observed by decreasing the water temperature by 2.0℃ and 4.0℃ every 12 hours and 24 hours in the range of 8.0-20.0℃. An unstable behavior pattern was observed in red seabream fingerlings exposed to water temperatures below 12.0℃, in which the swimming activity decreased and repeatedly stopped, regardless of the exposure time and water temperature fluctuation. The swimming ability of the organisms exposed to 8.0-10.0℃ decreased sharply, and the behavior of staying at the bottom of the test tank was observed. Only 50 % of the organisms survived due to the low-temperature stress, and all individuals died within six hours after the cold shock. In addition, the behavior index (BI) decreased rapidly, and the amplitude change of the coefficient of variation (CV) was found to have a greater variation than the other water temperatures (p<0.05). Low-temperature stress of red sea bream is promoted at 12.0℃, and it is interpreted as the tolerance limit, which can induce a sublethal response of the organisms exposed to cold shock of 8.0-10.0℃.

본 연구는 연속행동모니터링시스템(CBMS)을 사용하여 저온 충격에 노출된 참돔 Pagrus major 치어의 행동반응에 대한 임계 수온 및 행동 내성 반응을 규명하였다. 실험생물의 행동은 8.0~20.0℃ 수온구간에서 12시간과 24시간 마다 각각 2.0℃와 4.0℃씩 하강시키며 관찰하였다. 참돔 치어는 12.0℃ 이하로 수온이 하강한 경우 수온 노출시간과 변동 폭에 관계없이 유영활동이 감소하고 정지가 반복되는 불안정한 행동패턴이 관찰되었다. 또한 8.0~10.0℃ 수온에 노출된 생물의 유영능력은 급격히 저하되어 수조 바닥에서 머무르는 아치사 반응 행동을 보였다. 실험어류는 저 수온 스트레스 영향을 받아 생물의 50 %만 생존하였으며, 저온 충격(cold shock) 후 6시간 이내에 모든 개체가 사망하였다. 또한 행동지수(BI)는 급격히 감소하였으며 변이계수(CV)의 진폭변화는 타 수온보다 변동 폭이 큰 것으로 분석되었다(p<0.05). 참돔 치어의 저온 스트레스는 12.0℃를 경계로 촉진되며, 8.0~10.0℃의 저온 충격에 노출된 생물의 아치사 반응을 유발시키는 내성한계인 것으로 해석된다.

Keywords

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