Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Screening for Effective Organic Farming Materials for the Control of Tuber Rot of Gastrodia elata Caused by Fusarium spp.

천마 괴경썩음병 방제에 효과적인 유기농업자재 선발

  • 김창수 (전라북도농업기술원 약용자원연구소) ;
  • 이한나 (전라북도농업기술원 약용자원연구소) ;
  • 김효진 (전라북도농업기술원 농업환경과) ;
  • 서상영 (전라북도농업기술원 약용자원연구소) ;
  • 조종현 (전라북도농업기술원 약용자원연구소) ;
  • 이은숙 (전라북도농업기술원 약용자원연구소) ;
  • 안민실 (전라북도농업기술원 약용자원연구소) ;
  • 김희준 (전라북도농업기술원 연구개발국)
  • Received : 2021.07.01
  • Accepted : 2021.08.10
  • Published : 2021.11.30
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Abstract

This study was conducted to evaluate the control efficacy of the organic farming materials (OFMs) on tuber rot of Gastrodia elata caused by Fusarium spp. The antifungal activities in vitro as well as the suppressive effect of 15 OFMs on the spore germination and germ tube growth by inoculating spore suspension on immature tubers in vivo were investigated. 7 OFMs inhibited the mycelial growth of Fusarium spp. and 7 of them were microbial agents. In the screening using immature tubers, 3 OFMs were very effective with control efficacy value of 70%. Among them, sulfur provided suppressive effect on both mycelial growth and spore germination against tuber rot of G. elata. Finally, 3 OFMs were selected to test the protective and curative effects, and all chosen OFMs significantly suppressed disease incidence when applied in the preventive action, in comparison with the curative action. Especially, sulfur and Bacillus subtilis gave excellent protective control efficacy with control values of 93.2% and 86.9%, respectively, whereas its curative control effect was relatively low (73.3%, 60.2%). On the other hand, the preventive and curative effects of citronella + paraffin oil + ethyl alcohol were 73.3% and 67.0%, respectively. This study suggests that tuber rot of G. elata can be controlled by some OFMs in the rain shelter greenhouse under continuous cultivation condition and protective treatment is more important and efficient.

본 연구는 Fusarium spp.에 의해 발생하는 천마 괴경썩음병에 대하여 유기농업자재의 방제효과를 검정하기 위해 수행되었다. 유기농업자재 15품목을 대상으로 실내에서 항균활성 검정과 실내시설에서 천마 자마에 포자현탁액 접종을 통한 자마검정을 실시하였다. 이중 미생물제인 7품목의 유기농업자재가 Fusarium spp.의 균사생장을 억제하였다. 천마 자마를 이용한 검정에서 3품목의 유기농업자재가 70% 이상의 우수한 방제효과를 나타냈다. 그 중에서 sulfur가 유효성분인 광물질 제제의 억제효과가 가장 뛰어났다. 그 다음으로 3품목의 유기농업자재를 선발하여 예방적 효과와 치료적 효과를 검정하기 위해 적용하였는데, 예방적 처리를 했을 때 3품목 모두 병 발생에 대한 억제 효과가 상당히 높았다. 특히 sulfur와 B. subtilis의 예방적 효과는 각각 93.2%, 86.9%로 탁월하였으며, 치료적 효과는 73.3%, 60.2%로 상대적으로 낮게 나타났다. 반면, citronella + paraffin oil + ethyl alcohol은 예방적 효과와 치료적 효과가 각각 73.3%, 67.0%로 비슷한 효과를 나타냈다. 이 연구를 통해 시설하우스 내 연작 재배지에서 잘 발생하는 천마 괴경썩음병은 유기농업자재로 충분히 방제될 수 있으며, 예방적 처리가 더욱 중요하며 효과적임이 확인되었다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 지역특화기술개발 연구사업(PJ016156)의 지원에 의해 이루어진 것임.

References

  1. Agrios, G. N. 2005. Plant Pathology. 5th ed. Elsevier Academic Press, Amsterdam, The Netherlands.
  2. Beffa, T. 1993. Inhibitory action of elemental sulphur (S°) on fungal spores. Can. J. Microbiol. 39: 731-735. https://doi.org/10.1139/m93-107
  3. Cochrane, S. A. and J. C. Vederas. 2016. Lipopeptides from Bacillus and Paenibacillus spp.: a gold mine of antibiotic candidates. Med. Res. Rev. 36: 4-31. https://doi.org/10.1002/med.21321
  4. Emmert, E. A. and J. Handelsman. 1999. Biocontrol of plant disease: a (Gram-) positive perspective. FEMS Microbiol. Lett. 171: 1-9. https://doi.org/10.1016/S0378-1097(98)00570-9
  5. Han, M., M. N. Choi, H. R. Lee, and E. J. Park. 2017. First report of soft rot associated with Trichoderma hamatum in Gastrodia elata. Plant Dis. 101: 1048.
  6. Hong, S. J., Y. K. Kim, C. K. Shim, M. J. Kim, J. H. Park, E. J. Han, H. J. Jee, and S. C. Kim. 2015. Suppressive effect of organic farming materials on the development of tomato gray mold. Korean J. Organ. Agric. 23: 567-582. https://doi.org/10.11625/KJOA.2015.23.3.567
  7. Hsieh, M. T., C. R. Wu, and C. F. Chen. 1997. Gastrodin and p-hydroxybenzyl alcohol facilitate memory consolidation and retrieval but not acquisition on the passive avoidance task in rats. J. Ethnopharmacology 56: 45-54. https://doi.org/10.1016/S0378-8741(96)01501-2
  8. Jeong, H. K., H. J. Lee, and C. G. Kim. 2016. 2016 Production Status and Market Prospect of Eco-Friendly Agricultural Products at Home and Abroad. KREI Agricultural Policy Focus, No. 131. Korea Rural Economic Institute (KREI), Naju, Korea.
  9. Jules freund, M. D. 1951. The effect of paraffin oil and mycobacteria on antibody formation and sensitization. American J. Clinical Pathol. 21: 645-656. https://doi.org/10.1093/ajcp/21.7.645
  10. Katz, E. and A. L. Demain. 1977. The peptide antibiotics of Bacillus: chemistry, biogenesis, and possible functions. Bacteriol. Rev. 41: 449-474. https://doi.org/10.1128/br.41.2.449-474.1977
  11. Kim, C. S., H. J. Kim, K. T. Jeong, Y. M. Lee, B. H. Kim, and W. H. Lee. 2015. Technical development for shelter greenhouse and production of superior tubers in Gastrodia elata. R.D.A. Res. Rep. pp. 4-21.
  12. Kim, C. S., H. J. Kim, S. Y. Seo, M. S. Ahn, I. Y. Choi, H. N. Lee, H. J. Kim, and W. H. Lee. 2018. Tuber rot of Gastrodia elata by Fusarium spp. 2018 International Joint Conf. on Plant Protec. p. 278.
  13. Korea Crop Protection Association. 2020. Agrochemicals use guide book. www. koreacpa.org.
  14. Kwak, Y. K., I. S. Kim, M. C. Cho, C. S. Lee, and S. Kim. 2012a. Growth inhibition effect of environment-friendly farm materials in Colletotrichum acutatum in vitro. J. Bio-Environ. Control 21: 127-133.
  15. Kwak, Y. K., I. S. Kim, M. C. Cho, C. S. Lee, and S. Kim. 2012b. Growth inhibition effect of environment-friendly agricultural materials in Botrytis cinerea in vitro. J. Bio-Environ. Control 21: 134-139.
  16. Lee, M. U., J. Y. Lee, T. S. Lee, I. P. Hong, K. H. Ga, and K. J. Jang. 2007. Characteristics of important medicinal plant and new cultivation technology. Dongguk Univ. pp. 32-118.
  17. McCallan, S. E. A. and F. Wilcoxon. 1931. The fungicidal action of sulphur. II. The production of hydrogen sulphide by sulphured leaves and spores and its toxicity to spores. Contrib. Boyce Thompson Inst. 3: 13-38.
  18. Miller, L. P., S. E. A. McCallan, and R. M. Weed. 1953. Quantitative studies on the role of hydrogen sulfide formation in the toxic action of sulfur to fungus spores. Contrib. Boyce Thompson Inst. 17: 151-171.
  19. Nielsen, P. and J. Sorensen. 1997. Multi-target and medium independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere. FEMS Microbiol. Ecol. 22: 183-192. https://doi.org/10.1016/S0168-6496(96)00089-X
  20. Park, J. H., E. J. Han, H. G. So, and S. C. Kim. 2016. Effect of organic materials and the removal of apical shoot on controlling cucumber downy mildew. Korean J. Organ. Agric. 24: 919-929. https://doi.org/10.11625/KJOA.2016.24.4.919
  21. Park, J. S., W. J. Cho, and W. S. Kim. 2014. Selection and control effect of environmental friendly organic materials for controlling the main disease of Yuzu (Citrus junos Sieb). Korean J. Organ. Agric. 22: 115-127. https://doi.org/10.11625/KJOA.2014.22.1.115
  22. Park, S. H., S. J. Hong, C. K. Shim, M. J. Kim, J. H. Park, E. J. Han, and Y. K. Kim. 2017. Screening for effective organic farming materials for the control of cucumber scab caused by Cladosporium cucumerinum. Res. Plant Dis. 23: 159-167. https://doi.org/10.5423/RPD.2017.23.2.159
  23. Park, S. J., G. H. Kim, A. H. Kim, H. T. Lee, H. W. Gwon, J. H. Kim, K. H. Lee, and H. T. Kim. 2012. Controlling effect of agricultural organic materials on Phytophthora blight and anthracnose in red pepper. Res. Plant Dis. 18: 1-9. https://doi.org/10.5423/RPD.2012.18.1.001
  24. Park, S. M., J. S. Lee, C. D. Park, J. H. Lee, H. J. Jung, and T. S. Yu. 2006. Selection and antifungal activity of antagonistic bacterium Bacillus subtilis KMU-13 against cucumber scab, Cladosporium cucumerinum KACC 40576. Korean J. Biotechnol. Bioeng. 21: 42-48.
  25. Sung, J. E. and J. G. Jung. 2015. An analysis of the actual production conditions of organic farm. In: Advance Case Study and Development Strategy of Organic Agriculture, ed. by J. L. Cho. pp. 178-204. Korean Association of Organic Agriculture, Wanju, Korea.
  26. Tweedy, B. G. and N. Turner. 1966. The mechanism of sulfur reduction by conidia of Monilinia fructicola. Contrib. Boyce Thompson Inst. 23: 255-265.
  27. Zhang, S., W. Raza, X. Yang, J. Hu, Q. Huang, Y. Xu, X. Liu, W. Ran, and Q. Shen. 2008. Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer. Biol. Fertil. Soils 44: 1073. https://doi.org/10.1007/s00374-008-0296-0