Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidants of apple leaf extract

사과 잎(apple leaf) 추출물의 항산화 효과

  • 남승숙 (원광대학교 대학원 뷰티디자인과) ;
  • 고경숙 (원광대학교 뷰티디자인학부)
  • Received : 2020.09.03
  • Accepted : 2020.10.29
  • Published : 2020.10.31
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Abstract

In this study, in order to evaluate the possibility of applying apple leaf extract as a cosmetic material, we conducted an experiment by extracting with 70% ethanol. Apple leaf extract is analyzed by GC/MS, and toxicity is evaluated through polyphenol, flavonoid content and DPPH radical scavenging activity, antioxidant, and cell viability (MTT assay) OK, and nitric oxide (NO). It was confirmed that the NO production amount decreased 4.6 times in the group treated with the apology leaf extract through the measurement of the production inhibition of), and the anti-inflammatory effect was investigated. The total polyphenol content was 78.80 ± 0.25 mg /g and the total flavonoids were 65.25 ± 6.62 mg /g. It was confirmed that the DPPH scavenging activity increased sogonun at 79.8 ± 0.99,% at an extract concentration of 0.25%, 88.13 ± 0.89% at 0.5%, and 96.83 ± 2.00% at 1%. The cell viability was evaluated at 91.19 ± 3.49% even at a high concentration of 1000 ppm, confirming a viability of 80% or more. As a result of component analysis of GC-MS, it can be used as an antioxidant cosmetic material such as catechol (5.65%), DL-Gluciol (12.05%), Ascorbic acid (2.41%), Phytol (13.88%), Hexanoic acid (5.47%). Conceivable. The result of this experiment will be used as an important basic material in the development of natural functional cosmetic materials using apple leaf extract.

본 연구는 사과 잎 추출물의 화장품 소재로 응용하기 위한 가능성을 평가하기 위하여 에탄올 70%로 추출하여 실험을 진행하였다. 사과 잎 추출물을 GC/MS로 분석하였고, 폴리페놀, 플라보노이드 함량과 DPPH radical 소거 활성을 통하여 항산화, 세포생존율(MTT assay) 확인을 통한 독성 평가를 하였으며, Nitric oxide (NO) 생성 저해 측정을 통해 사과 잎 추출물을 처리한 군에 4.6배의 NO 생성량이 감소하는 것을 확인하였고, 항염효과를 알아보았다. 총 폴리페놀 함량은 78.80 ± 0.25 mg/g, 총 플라보노이드 65.25±6.62 mg/g로 나타났다. DPPH radical 소거 활성은 추출물 농도 0.25%에서 79.8± 0.99%, 0.5%에서 88.13±0.89%, 1%에서 96.83±2.00%로 소거능이 증가함을 확인하였다. 세포생존율 평가는 고농도인 1000 ppm 농도에서도 91.19±3.49%로 80% 이상의 생존률을 확인하였다. GC-MS 성분분석 결과 catechol(5.65%), DL-Gluciol(12.05%), Ascorbicacid(2.41%), Phytol(13.88%), Hexanoic acid(5.47%) 등 항산화 화장품 소재로 활용이 사료된다. 이에 실험 결과 사과 잎 추출물을 이용한 천연 기능성 화장품 소재 개발에 있어 중요한 기초 자료로 이용되고자 한다.

Keywords

References

  1. H. A, Yu, C. D, Kim "Applicability of Lindera obtusiloba Flower Extracts as Cosmetic Ingredients". Asian journal of beauty and cosmetology, Vol.15, No.2 pp. 132-144, (2017) https://doi.org/10.20402/ajbc.2016.0088
  2. Y. M, Yun, Y. S, Han, "Effect of Anti-oxidant Activity and the Skin Whitening Action on Plantago asiatica L. Root Extract" Korean Society for Biotechnology and Bioengineering Journal, Vol.29, No.3 pp. 199-204, (2014)
  3. D. S. An, S. J. Seo, N. W. Kim, Y. S. Lee, "Anti-aging and Anti-inflammatory Activity of Rhus javanica Branches Extracts", Journal of Investigative Cosmetology, Vol. 13, No.2 pp. 103-111, (2017) https://doi.org/10.15810/jic.2017.13.2.002
  4. M. K. Kim "Comparison of Antioxidant, Antimicrobial Activity and Anti-inflammatory effects of on Neem (Azadirachta indica) Extracts with Different Extraction Methods", Journal of the Korea Soc. Beauty and Art, Vol.20, No.3 pp. 265-278, (2019) https://doi.org/10.18693/jksba.2019.20.3.265
  5. M. S. Seo, Y.A. J. Jin-T. Lee. "The Study of Cosmeceutical Activities from Lentinula edodes extracts and Application a Natural Cosmetic Material", Journal of Oil &Applied Science, Vol.35, No.4 pp. 1003-1012, (2018) https://doi.org/10.12925/JKOCS.2018.35.4.1003
  6. S. h. Ko, Y. S. Kim, J. H. Lee, I. H. Kim, S. B. Kim, K. B. Noh, S. W. Shin, E. S. Jung, D. H. Park. "Functional Characterization of Callus Extracts of Apple 'Hirosaki' for Cosmetic Materials" Korean society for biotechnology and bioengineering journal, Vol.28, No.4 pp. 244-248, (2013)
  7. I.R. Kim "Inhibitory Effects of Apple Peel Extract on Inflammatory Enzymes", Korean journal of food science and technology, Vol.47. No.4, pp. 534-538, (2015) https://doi.org/10.9721/KJFST.2015.47.4.534
  8. S. h. Ko, Y. S. Kim, J. H. Lee, I. H. Kim, S. B. Kim, K. B. Roh, S. W. Shin, E.S Jung, D. h. Park. "Functional Characterization of Callus Extracts of Apple 'Hirosaki' for Cosmetic Materials", Korean society for biotechnology and bioengineering journal, Vol.28, No.4 pp. 244-248, (2013)
  9. www.kcia.or.kr Pyrus Malus (Apple) Leaf Extract CAS NO 85251-63-4
  10. S. J. Choi, E.A. Cho, E. H. Cho, Y. J. Jeong, C. S. Ku, B. J. Ha, H. J. Chae, "Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract", Korean Society for Biotechnology and Bioengineering Journal, Vol.26. No.2 pp. 165-171, (2011)
  11. J. A. Park "Antioxidant effects of Rumex crispus L. leaf extracts and protective effects on Human HaCaT Keratinocyte", Journal of the Korea Soc. Beauty and Art, Vol. 12. No.2 pp.189-198, (2011)
  12. S. h. You. J. S. Moon, "A Study on Anti-oxidative, Anti-inflammatory, and Melanin Inhibitory Effects of Chrysanthemum Sibiricum Extractv", Journal of oil & applied science, Vol.33, No.4 pp. 762-770, (2016) https://doi.org/10.12925/jkocs.2016.33.4.762
  13. J. Kim, J. G. Park, C. M. Lee, S. G. Kim. "Effect of the Pine Cone Extract Phytochemical and Physiological activity on HaCaT Cells", Asian journal of beauty and cosmetology, Vol.17, No,3 pp. 353-363, (2019) https://doi.org/10.20402/ajbc.2019.0303
  14. J. S. Park, I. h. Han. "Effect of Extraction Solvent on the Physiological Properties of Korean Pear Peel (Pyrus pyrifolia cv. Niitaka)", Korean Society of Food Science and Technology, Vol.47, No.2, pp. 254-260, (2015) https://doi.org/10.9721/KJFST.2015.47.2.254
  15. K. S. Ko, "A Study on the Antioxidant Activity and Cytotoxicity of Scutellaria baicalensis Extracts", Journal of the Korea Soc Beauty and Art, Vol.14, No.2 pp. 233-249, (2013)
  16. I. R. Kim, "Inhibitory Effects of Apple Peel Extract on Inflammatory Enzymes", Korean journal of food science and technology, Vol.47, No.4, pp. 534-538, (2015) https://doi.org/10.9721/KJFST.2015.47.4.534
  17. K. S. Ko. "A Study on Antioxidant Effect of Methanol Extract from Viola Mandshurica", Journal of the Korean Society of Cosmetology, Vol.18, No.5 pp. 1082-1086, (2012)
  18. S. h. You, J. S. Moon, "A Study on Anti-oxidative, Anti-inflammatory, and Melanin Inhibitory Effects of Chrysanthemum Sibiricum Extract", Journal of oil & applied science, Vol.33, No.4, pp. 762-770, (2016) https://doi.org/10.12925/jkocs.2016.33.4.762
  19. K. S. Ko. "A Study on the Antioxidant Activity and Cytotoxicity of Scutellaria baicalensis Extracts", Journal of the Korea Soc. Beauty and Art, Vol.14, No.2 pp. 233-249, (2013)
  20. S. J. Choi1, E. A. Cho, E. H. Cho1, Y. J. Jeong, C. S. Ku, B. J. Ha, H. J. Chae, "Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract", Korean Society for Biotechnology and Bioengineering Journal, Vol.26, No.2 pp. 165-171, (2011)
  21. I. R. Kim, "Inhibitory Effects of Apple Peel Extract on Inflammatory Enzymes", Korean journal of food science and technology. Vol.47 No. 4, pp. 534-538, (2015) https://doi.org/10.9721/KJFST.2015.47.4.534
  22. N. Y. Kim, J. H. Kim, G. P. Choi, H. Y. Lee, "Comparison of Anti-Skin Wrinkle Activities of Aronia melanocarpa Extracts by Extraction Methods", Korean Jnalour of Medicinal Crop Science, Vol.22, No.3 pp. 217-222, (2014) https://doi.org/10.7783/KJMCS.2014.22.3.217
  23. H. D. Kwon, J. H. Park, J, Park, K. S .Cha, W. K. Lee, S. T. Ha, "Antitumer Activity and Substantial Identification of the Solvent Extracts from pulsatilla koreana,clematis florida and ulmus davidiana", Pusan. Institute Health& Environ. Vol.7, pp. 56-73, (1997)
  24. M. J. Kim, Y. G. Kim, H. S. Kim, C. Cheong, K. H. Jang, S. A. Kang, "Effects of Antioxidant Activities in Ethanol Extract of Apple Peel, Grape Peel, and Sweet Potato Peel as Natural Antioxidant", Journal of the Korea Academia-Industrial, Vol.15, No.6 pp. 3766-3773, (2014)