Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Growth Characteristics, Main Constituents and Antioxidant Activities in Local Accessions of Sophora flavescens AIT.

고삼 수집종의 생육특성, 주요성분 및 항산화 활성

  • Kwon, A Reum (Ginseng Medicinal Plant Research Institute, CNARES) ;
  • Kim, Sun Ick (Ginseng Medicinal Plant Research Institute, CNARES) ;
  • Seong, Bong Jae (Ginseng Medicinal Plant Research Institute, CNARES) ;
  • Jee, Moo Geun (Ginseng Medicinal Plant Research Institute, CNARES) ;
  • Lee, Ka Soon (Ginseng Medicinal Plant Research Institute, CNARES) ;
  • Kim, Hyun Ho (Ginseng Medicinal Plant Research Institute, CNARES) ;
  • Doh, Eun Soo (Department of Herbal Health Pharmaceutical Science, Joongbu University)
  • 권아름 (충청남도농업기술원 인삼약초연구소) ;
  • 김선익 (충청남도농업기술원 인삼약초연구소) ;
  • 성봉재 (충청남도농업기술원 인삼약초연구소) ;
  • 지무근 (충청남도농업기술원 인삼약초연구소) ;
  • 이가순 (충청남도농업기술원 인삼약초연구소) ;
  • 김현호 (충청남도농업기술원 인삼약초연구소) ;
  • 도은수 (중부대학교 한방보건제약학과)
  • Received : 2020.08.10
  • Accepted : 2020.09.29
  • Published : 2020.10.30
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Abstract

Background: Sophora flavescens AIT root is used as a herbal medicine in Asian culture. This study was aimed at selecting the best cultivars, by comparing growth characteristics, active ingredients, and antioxidant activities. Methods and Results: A total twenty nine accessions of S. flavescens were collected from five different regions in Korea. Plant height, leaf length, peduncle length, fresh root weight, and seed weight were the growth characteristics selected. Antioxidant activity was determined by measuring the total polyphenol (TPC) and total flavonoid contents (TFC) as well as the radical scavenging activity of DPPH, ABTS and FRAP. Oxymatrine and matrine, active ingredients in S. flavescens were analyzed by HPLC. Results indicated that accession YS-11 had the highest plant height (152.5 cm) and peduncle length (54.0 cm). The fresh root weight was highest in JA-01 at 4.9 kg, while the highest total seed weight was recorded in accession BH-04 at 77.7 g. The YS-03 accession contained the highest oxymatrine and matrine total contents (0.9616% and 0.9638%, respectively). Accessions BH-02, YS-11, YS-05, and MJ-03 had the highest levels of antioxidant activity (p < 0.05). Oxymatrine showed a high correlation with TPC and FRAP. TPC showed high correlations with TFC, DPPH, ABTS and FRAP. TFC showed a high correlation with DPPH. Conclusions: The superior lines can be selected for use as standard variety breeding material by comparing the growth characteristics of the accessions.

Keywords

References

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