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http://dx.doi.org/10.7783/KJMCS.2020.28.5.360

Growth Characteristics, Main Constituents and Antioxidant Activities in Local Accessions of Sophora flavescens AIT.  

Kwon, A Reum (Ginseng Medicinal Plant Research Institute, CNARES)
Kim, Sun Ick (Ginseng Medicinal Plant Research Institute, CNARES)
Seong, Bong Jae (Ginseng Medicinal Plant Research Institute, CNARES)
Jee, Moo Geun (Ginseng Medicinal Plant Research Institute, CNARES)
Lee, Ka Soon (Ginseng Medicinal Plant Research Institute, CNARES)
Kim, Hyun Ho (Ginseng Medicinal Plant Research Institute, CNARES)
Doh, Eun Soo (Department of Herbal Health Pharmaceutical Science, Joongbu University)
Publication Information
Korean Journal of Medicinal Crop Science / v.28, no.5, 2020 , pp. 360-370 More about this Journal
Abstract
Background: Sophora flavescens AIT root is used as a herbal medicine in Asian culture. This study was aimed at selecting the best cultivars, by comparing growth characteristics, active ingredients, and antioxidant activities. Methods and Results: A total twenty nine accessions of S. flavescens were collected from five different regions in Korea. Plant height, leaf length, peduncle length, fresh root weight, and seed weight were the growth characteristics selected. Antioxidant activity was determined by measuring the total polyphenol (TPC) and total flavonoid contents (TFC) as well as the radical scavenging activity of DPPH, ABTS and FRAP. Oxymatrine and matrine, active ingredients in S. flavescens were analyzed by HPLC. Results indicated that accession YS-11 had the highest plant height (152.5 cm) and peduncle length (54.0 cm). The fresh root weight was highest in JA-01 at 4.9 kg, while the highest total seed weight was recorded in accession BH-04 at 77.7 g. The YS-03 accession contained the highest oxymatrine and matrine total contents (0.9616% and 0.9638%, respectively). Accessions BH-02, YS-11, YS-05, and MJ-03 had the highest levels of antioxidant activity (p < 0.05). Oxymatrine showed a high correlation with TPC and FRAP. TPC showed high correlations with TFC, DPPH, ABTS and FRAP. TFC showed a high correlation with DPPH. Conclusions: The superior lines can be selected for use as standard variety breeding material by comparing the growth characteristics of the accessions.
Keywords
Sophora flavescens AIT.; Accessions; Antioxidant; Matrine; Oxymatrine;
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Times Cited By KSCI : 19  (Citation Analysis)
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1 Huang KC. (1998). The pharmacology of Chinese herbs. CRC Press. Boca Raton. FL, USA. p.63-66.
2 Jin JH, Kim JS, Kang SS, Son KH, Chang HW and Kim HP. (2010). Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens. Journal of Ethnopharmacology. 127:589-595.   DOI
3 Jin L, Cui Y and Zhang G. (1993). HPLC analysis of alkaloids in Sophora Flavescens Ait. Acta Pharmaceutica Sinica. 28:136-139.
4 Jung YJ, Assefa DA, Lee JE, Lee HS, Rhee JH and Sung JS. (2019). Analysis of antioxidant activity and serotonin derivatives in Safflower(Carthamus tinctorius L.) germplasm collected from five countries. Korean Journal of Plant Resources. 32:423-432.
5 Kang SA, Han JA, Choue RW and Jang KH. (2004). DPPH radical scavenger activity and antioxidant effects of Cham-Dang-Gui(Angelica gigas). Journal of the Korean Society of Food Science and Nutrition. 33:1112-1118.   DOI
6 Kang SS and Kim JS. (2004). Isolation and quantitative determination of matrine from Sophorae Radix. Korean Journal of Crop Science. 49:155-161.
7 Kang TL and Hwang GS. (1992). Studies on bioresponses of Sophorae Radix(1). Journal of Haehwa Medicine. 1:47-55.
8 Kil YS, Sin SM, Lee DY, Min JW, Yang KJ, Lee SW, Kim YH and Goo YM. (2018). Analysis of triterpene glycoside levels and antioxidant activity in the different shoot tissues of Centella asiatica(L.) Urban. Journal of Life Science. 28:917-922.   DOI
9 Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH and Lee BH. (2012). Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean Journal of Food Science and Technology. 44:337-342.   DOI
10 Kim EJ, Lee HJ, Kim HJ, Nam HS, Lee MK, Kim HY, Lee JH, Kang YS, Lee JO and Kim HY. (2005). Comparison of colorimetric methods for the determination of flavonoid in propolis extract products. Korean Journal of Food Science and Technology. 37:918-921.
11 Kim EO, Lee KT and Choi SW. (2008a). Chemical comparison of germinated- and ungerminated-safflower(Carthamus tinctorius) seeds. Journal of the Korean Society of Food Science and Nutrition. 37:1162-1167.   DOI
12 Kim HJ and Chae YA. (2001). Variation of chamazulene contents in local collections of Mugwort(Artemisia spp.). Korean Jorunal of Medicinal Crop Science. 9:181-185.
13 Kim HJ, Jung SH, Bae JH and Lee SY. (2008b). Growth characteristics, vitamin C content and antioxidative activity among local strains of Sedum sarmentosum. Journal of Bio-Environment Control. 17:110-115.
14 Kim KH and Jung HA. (2014). Clinical study for the efficacy of external preparation containing Sophorae Radix, Aurantii Immaturus Fructus, Schizonepetae Spica, Forsythia fructus and Portulacea Herba extract on atopic dermatitis. The Journal of Korean Medical Ophthalmology and Otolaryngology and Dermatology. 27:17-36.   DOI
15 Lee BC, Paik SW, Kim SD, Yun TS, Park JS and Kwak TS. (1999). Growth characteristics and yield of collected boxthon (Lycium chinense Mill.) varieties. Korean Journal of Medicinal Crop Science. 7:147-154.
16 Kim KS, Park CG and Bang JK. (2003). Varietal and yearly differences of lignan contents in fruits of collected lines of Schizandra Chinensis Baillon. Korean Journal of Medicinal Crop Science. 11:71-75.
17 Kim YK, Lee JJ and Choi MY. (2008c). Insecticidal activities and repellent effects of plant extracts against the Brown Planthopper(Nilaparvata lugens Stal). Korean Journal of Applied Entomology. 47:65-74.   DOI
18 Kim YS and Lee KJ. (2009). Extraction of genistein and formononetin from Sophoraflavescens Aiton using ultrasonic wave. Korean Chemical Engineering Research. 47:258-261.
19 Kumagai H, Masuda T, Ohsono M, Hattori S, Naganawa H, Sawa T, Hamada M, Ishizuka M and Takeuchi T. (1990). Cytogenin, a novel antitumor substance. The Journal of Antibiotics. 43:1505-1507.   DOI
20 Kumar A, Pant P, Basu S, Rao GRK and Khanna HD. (2007). Oxidative stress in neonatal hyperbilirubinemia. Journal of Tropical Pediatrics. 53:69-71.   DOI
21 Lee GS, Kim ES, Cho SI, Kim JH, Choi GY, Ju YS, Park SH, Jeong SI and Kim HJ. (2010). Antibacterial and synergistic activity of prenylated chalcone isolated from the roots of Sophora flavescens. Journal of the Korean Society for Applied Biological Chemistry. 53:290-296.   DOI
22 Lee HJ, Lee JH, Jung JT, Lee YJ, Oh MW, Chang JK, Jeong HS and Park CG. (2019). Changes in free sugar, coixol contents and antioxidant activities of Adlay sprout(Coix lacryma-jobi L. var. ma-yuen Stapf.) according to different growth stage. Korean Journal of Medicinal Crop Science. 27:339-347.   DOI
23 Ma L, Wen S, Zhan Y, He Y, Liu X and Jiang J. (2008). Anticancer effects of the Chinese medicine matrine on murine hepatocellular carcinoma cells. Planta Medica. 74:245-251.   DOI
24 Lee HO. (2001). Anticaries effect and cytotoxicity of extract of Sophora flavescens Ait. Journal of Korean Academy of Oral Health. 25:333-345.
25 Lee MH, Kim SE, Kim YS, Lee HK, Lee HG, Jee HJ, Kim YK, Shim CK, Kim MJ, Hong SJ and Lee YS. (2013). Studies on the eco-friendly management of Whiteflies on organic tomatoes with oleic acid. Korean Journal of Organic Agriculture. 21:95-104.   DOI
26 Lee MY, Yoo MS, Whang YJ, Jin YJ, Hong MH and Pyo YH. (2012). Vitamin C, total polyphenol, flavonoid contents and antioxidant capacity of several fruit peels. Korean Journal of Food Science and Technology. 44:540-544.   DOI
27 Lee SE, Kim JU, Jeong HS, Choi JH, Ji YJ, Kim HD, Jang GY, Hyun DY and Kim DH. (2020). N-methyl-D-aspartate (NMDA) receptor antagonistic effect and ginsinoside content of Panax ginseng C. A. Meyer cultivar root extract. Korean Journal of Medicinal Crop Science. 28:9-20.   DOI
28 Liu ZL, Goh SH and Ho SH. (2007). Screening of Chinese medicinal herbs for bioactivity against Sitophilus zeamais motschulsky and Tribolium castaneum(Herbst). Journal of Stored Products Research. 43:290-296.   DOI
29 Mao L and Henderson G. (2007). Antifeedant activity and acute and residual toxicity of alkaloids from Sophora flavescens (Leguminosae) against formosan subterranean termites(Isoptera: Rhinotermitidae). Journal of Economic Entomology. 100:866-870.   DOI
30 Ministry of Food and Drug Safety(MFDS). (2019). The Korean pharmacopoeia IV. Ministry of Food and Drug Safety. Cheongju, Korea. p.14-15.
31 Ministry of Food and Drug Safety(MFDS). (2020). The Korean herbal pharmacopoeia III. Ministry of Food and Drug Safety. Cheongju, Korea. p.28-29.
32 Moon JW, Jang IB, Yu J, Jang IB, Seo SJ and Lee SW. (2019). Changes in growth characteristics, biological activity and active compound contents in ginseng of different ages. Korean Journal of Medicinal Crop Science. 27:383-389.   DOI
33 Mou JC, Lee SN, Kim MG, Kim MH, Kim HJ, Jo HJ and Leem KH. (2011). Effects of astragali radix extracts on the elastase activity and DPPH and NO scavenging activities. Korean Journal of Herbology. 26:59-63.
34 Mujic I, Sertovic E, Jokic S, Saric Z, Alibabic V, Vidovic S and Zivkovic J. (2011). Isoflavone content and antioxidant properties of soybean seeds. Croatian Journal of Food Science and Technology. 3:16-20.
35 Perry LM and Metzger J. (1980). Medicinal plants of east and southeast Asia: Attributed properties and uses. The MIT Press. Cambridge. MA, USA. p.226.
36 National Forest Seed and Variety Center(NFSV). (2016). Investigation of characteristics of crop for screening new varieties-Shrubby sophora. National Forest Seed and Variety Center. Chungju, Korea.
37 Ota N and Mino Y. (1979). Pharmacognostical studies on the Chinese crude drug "Sophorae radix". I. High-speed liquid chromatographic studies on the alkaloid constituents of Sophorae radix. Shoyakugaku Zasshi. 33:140-145.
38 Park SJ, Kim SC and Lee JR. (2010). Antimicrobial effects of Sophorae Radix extracts against oral microorganisms. The Korea Journal of Herbology. 25:81-88.
39 Park SY, Nam YJ and Kim DH. (2002). The dyeability and antimicrobial activity of Sophora Radix ethanol extracts: Characteristics of dyed Silk. Textile Coloration and Finishing. 14:1-10.
40 Park YJ, Seong JD, Kim HY, Suh HS and Lee SK. (1994). Root yield and saikosaponin content in local strains of Bupleurum falcatum L. Korean Journal Crop Science. 39:453-457.
41 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237.   DOI
42 Rho TH, Seo KS and Shim JS. (1994). Growth characteristics and content of chemical components in shade-cultured Artemisia spp. collected from different areas. Korean Journal of Medicinal Crop Science. 2:205-210.
43 Ryu SY, Lee HS, Kim YK and Kim SH. (1997). Determination of isoprenyl and lavandulyl positions of flavonoids from Sophora flavescens by NMR experiment. Archives of Pharmacal Research. 20:491-495.   DOI
44 Tang W and Eisenbrand. (1992). Chinese drugs of plant origin. Chemistry, pharmacology, and use in traditional modern medicine. Chinese Drugs of Plant Origin. Springer. Berlin, Germany. p.931-943.
45 Shi W, Wang Y, Li J, Zhang H and Ding L. (2007). Investigation of ginsenosides in different parts and ages of Panax ginseng. Food Chemistry. 102:664-668.   DOI
46 Shin MK. (2000). Extract of Sophorae Radix for the prevention and treatment of osteoporosis. Korea. Patent. 1020000073825.
47 Sohn HY, Son KH, Kwon CS, Kwon GS and Kang SS. (2004). Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera(L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine. 11: 666-672.   DOI
48 Song J, Xu H, Tian S and But PPH. (1999). Determination of quinolizidine alkaloids in traditional Chinese herbal drugs by nonaqueous capillary electrophoresis. Journal of Chromatography A. 857:303-311.   DOI
49 Szakiel A, Paczkowski C and Henry M. (2011). Influence of environmental abiotic factors on the content of saponins in plants. Phytochemistry Review. 10:471-491.   DOI
50 Tungmunnithum D, Thongboonyou A, Pholboon A and Yangsabai A. (2018). Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines. 5:93. https://www.mdpi.com/2305-6320/5/3/93 (cited by 2020 April 25).   DOI
51 Woo ER, Kwak JH, Kim HJ and Park HK. (1998). A new prenylated flavonol from the roots of Sophora flavescens. Journal of Natural Products. 61:1552-1554.   DOI
52 Wu LI, Miyase T, Ueno A, Kuroyanagi M, Noro T and Fukushima S. (1985). Studies on the Constituents of Sophora flavescens AITON. II. Chemical and Pharmaceutical Bulletin. 33:3231-3236.   DOI
53 Chen C, Guo SM and Liu B. (2000). A randomized controlled trial of kurorinone versus interferon-${\alpha}2a$ treatment in patients with chronic hepatitis B. Journal of Viral Hepatitis. 7:225-229.   DOI
54 Ambriz-Perez DL, Leyva-Lopez N, Cutierrez-Grijava EP and Heredia JB. (2016). Phenolic compounds: Natural alternative in inflammation treatment. A Review. Cogent Food and Agriculture. 2:1131412. https://doi.org/10.1080/23311932.2015.1131412 (cited by 2020 Aug 1).
55 Yagi A, Fukunaga M, Okuzako N, Mifuchi I and Kawamoto F. (1989). Antifungal substances from Sophora flavescens. Shoyakugaku Zasshi. 43:343-347.
56 An TH, Lee SY and Cho JW. (2012). Comparison on antioxidant activity of ethanolic extracts of Chinese matrimony vine(Lycium chinensis M.). Korean Journal of Crop Science. 57:22-29.   DOI
57 Animal and Plant Quarantine Agency(APQA). (2019). Year book of plant quarantine statistics. Animal and Plant Quarantine Agency. Gimcheon, Korea. p.233.
58 Benzie IFF and Strain JJ. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant powder": The FRAP assay. Analytical Biochemistry. 239:70-76.   DOI
59 Brand-Williams W, Cuvelier ME and Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology. 28:25-30.   DOI
60 Chang YH, Park CG and Kim DH. (1995). Characteristics of flower and fruit in collected Schizandra chinensis BAILLON. Korean Journal of Medicinal Crop Science. 3:35-39.
61 Chen X, Yi C, Yang X and Wang X. (2004). Liquid chromatography of active principles in Sophora flavescens root. Journal of Chromatography B. 812:149-163.   DOI
62 Cho H, Weon SR, Yang EY, Kim JS, You IS, Ru DG, Lee JH, Kang KU and Baek SH. (1999). Antimicrobial effect of the extract of Sophora flavescens Ait(I). Yakhak Hoeji. 43:419-422.
63 Zheng Y, Yao J and Shao X. (2000). Review on the constituents and agricultural application of Sophora flavescens Ait. Pesticide Science Administration. 21:24-26.
64 Yang SK, Yun YJ and Namgung MO. (2004). Determination of oxymatrine in Sophora Radix by high performance liquid chromatography. Analytical Science and Technology. 17:289-294.
65 Yang X and Zhao B. (2006). Antifungal activities of matrine and oxymatrine and their synergetic effects with chlorthalonil. Journal of Forestry Research. 17:323-325.   DOI
66 Zhang L, Xu L, Xiao S, Liao Q, Li Q, Liang J, Chen X and Bi KS. (2007). Characterization of flavonoids in the extract of Sophora flavescens Ait. by high-performance liquid chromatography coupled with diode-array detector and electrospray ionization mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 44:1019-1028.   DOI
67 Coseteng MY and Lee CY. (1987). Changes in apple polyphenoloxidase and polyphenol concentrations in relation to degree of browning. Journal of Food Science. 52:985-989.   DOI
68 Cho JH, Lee JY, Lee MJ, Oh HN, Kang DH and Jhune CS. (2013). Comparative analysis of useful $\ss$-glucan and polyphenol in the fruiting bodies of Ganoderma spp. Journal of Mushroom Science and Production. 11:164-170.   DOI
69 Choi JN, Lee HJ, Lee YJ, Jeong JT, Lee JH, Chang JK and Park CG. (2020). Growth characteristics and asiaticoside content, and antioxidant activities in Centella asiatica by cultivation and irrigation methods. Korean Journal of Medicinal Crop Science. 28:254-259.   DOI
70 Chung IM and Kim SH. (2004). Analysis methods of phenol compounds. Korean Journal of Crop Science. 49:43-50.
71 Cuvelier ME, Richard H and Berset C. (1996). Antioxidative activity and phenolic composition of pilot plant and commercial extracts of sage and rosemary. Journal of the American Oil Chemists' Society. 73:645-652.   DOI
72 Ding PL, Liao ZX, Huang H, Zhou P and Chen DF. (2006). (+)-$12{\alpha}$-Hydroxysophocarpine, a new quinolizidine alkaloid and related anti-HBV alkaloids from Sophora flavescens. Bioorganic and Medicinal Chemistry Letters. 16:1231-1235.   DOI
73 Ding Y, Tian RH, Kinjo J, Nohara T and Kitagawa I. (1992). Three new oleanene glycosides from Sophora flavescens. Chemical and Pharmaceutical Bulletin. 40:2990-2994.   DOI
74 Ha H, Lee YS, Lee JH, Choi HS and Kim CS. (2006). High performance liquid chromatographic analysis of isoflavones in medicinal herbs. Archives of Pharmacal Research. 29:96-101.   DOI
75 Han SH, Jang JK, Ma KH, Kim YJ, Kim SM, Lee HJ and Hong CO. (2019). Selection of superior resources through analysis of growth characteristics and physiological activity of Schisandra chinensis collection. Korean Journal of Medicinal Crop Science. 27:9-16.   DOI