Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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The Effects of Gobonyangjeonbang Administration on Antioxidative of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced Rats.

고본양정방 투여가 TCDD 유도 랫드의 항산화 변화에 미치는 영향

  • Yang, Dong Hyun (Department of Biomedical Laboratory Science, Daejeon University) ;
  • OH, Ji Hye (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Park, Un kyu (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Cho, Chung Sik (Department of Korean Medicine, Daejeon University) ;
  • Hwang, Seock Yeon (Department of Biomedical Laboratory Science, Daejeon University)
  • 양동현 (대전대학교 임상병리학과) ;
  • 오지혜 (대전대학교 임상병리학과) ;
  • 박운규 (대전대학교 임상병리학과) ;
  • 조충식 (대전대학교 한의과대학) ;
  • 황석연 (대전대학교 임상병리학과)
  • Received : 2020.07.03
  • Accepted : 2020.10.05
  • Published : 2020.10.31
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Abstract

The purpose of this study was to evaluate the effects of gobonyangjeonbang (GYB) on the endocrine function and the antioxidant efficacy of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced oxidative stress in rats. In 2017, to evaluate the efficacy of GYB on oxidative stress, 35 male SD rats were divided into five groups and tested. The normal control group was administered saline as a vehicle, while the TCDD-alone group was administered TCDD (2 ㎍/kg per week) intraperitoneally and with physiological saline, and the test group was administered GYB orally by dividing it into three concentrations (75, 150, and 300 mg/kg) for six weeks. Bodyweight decreased significantly after six weeks of TCDD exposure, when compared to rats in the NC group (p<0.001). However, weight loss from TCDD was significantly protected by administration of GYB at 300 mg/kg (p<0.01). The rat liver induced by TCDD showed cytoplasmic vacuole degeneration, and the hepatic sinusoid and weight increased. As a result of measuring MDA and SOD, both items tended to decrease under TCDD administration. On the other hand, there was no change due to GYB administration, and significance was observed in the GYB 300 mg/kg group compared to the NC group in the SOD result (p<0.05). These findings demonstrated that GYB may have a protective effect against TCDD-induced liver toxicity in rats.

본 연구의 목적은 TCDD로 인해 발생하는 산화적 스트레스에 대한 GYB의 내분비 기능 및 항산화 효능에 미치는 영향을 평가하기 위한 것이다. 2017년도에, 산화적 스트레스에 대한 GYB의 효능을 평가하기 위해 35마리의 수컷 SD랫드를 5개의 그룹으로 나누어 실험하였다. 정상 대조군은 vehicle 및 saline을 투여하였으며, TCDD 단독투여그룹은 TCDD(2 ㎍/kg, weeks) 및 생리식염수를 복강 내 투여하였으며, 시험 군은 6주 동안 GYB (75, 150, 300 mg/kg)를 3가지 농도로 나누어 경구로 투여하였다. 체중은 NC 그룹의 랫드와 비교하였을 때 TCDD 노출 후 6주 후에 유의하게 감소하였다(p<0.001). 그러나 TCDD에 의한 체중 감소는 GYB 300 mg/kg 투여에 의해 현저하게 보호되었다(p<0.01). TCDD로 유도된 랫드의 간은 세포질의 공포 변성이 나타났으며, 간의 동양 혈관과 중량의 증가를 나타냈다. MDA, SOD를 측정한 결과, TCDD 투여에 의하여 두 항목 모두 감소하는 경향이 나타났다. 반면, GYB 투여에 의한 변화는 나타나지 않았고, SOD 결과에서 GYB 300군에서 NC군 대비 유의성이 관찰(p<0.05) 되었다. 이러한 발견은 GYB가 랫드에서 TCDD 유도 간 독성에 대해 보호 효과를 가질 수 있음을 증명하였다.

Keywords

References

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