Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Elephant Garlic Extracts Inhibit Adipogenesis in 3T3-L1 Adipocytes

코끼리마늘의 3T3-L1 지방세포 분화억제 효과

  • Lee, Seul Gi (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Hahn, Dongyup (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Soo Rin (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Won Young (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Nam, Ju-Ock (Department of Food Science and Biotechnology, Kyungpook National University)
  • 이슬기 (경북대학교 식품공학부) ;
  • 한동엽 (경북대학교 식품공학부) ;
  • 김수린 (경북대학교 식품공학부) ;
  • 이원영 (경북대학교 식품공학부) ;
  • 남주옥 (경북대학교 식품공학부)
  • Received : 2019.11.25
  • Accepted : 2020.04.27
  • Published : 2020.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Elephant garlic (Allium ampeloprasum L.) has been reported to have several pharmacological effects. However, its anti-adipogenic effect and the possible molecular mechanisms have not yet been reported. In this study, we demonstrate that elephant garlic extracts suppress adipogenesis in 3T3-L1 adipocytes. Raw and steamed elephant garlic extracts (REG and SEG, respectively) suppressed the differentiation of adipocytes and cellular lipid accumulation. Of note, the anti-differentiation effect of REG treatment on 3T3-L1 cells resulted in cytotoxicity, whereas SEG-treated cells displayed no such cytotoxicity. Additionally, SEG treatment significantly reduced the adipogenesis-related gene expression of PPAR γ, C/EBPα, adiponectin, Ap2, and LPL. To our knowledge, these results are the first evidence of the anti-adipogenic effects of elephant garlic extracts on 3T3-L1 adipocytes.

부추 속에 속하는 코끼리마늘(Allium ampeloprasum L.)에 대한 몇 가지 약리효과는 보고된 바 있으나, 이의 항비만 및 작용기전에 대한 보고 전무한 실정이다. 이에 따라, 본 연구에서는 코끼리마늘 추출물이 3T3-L1 지방세포의 분화에 미치는 영향을 조사하여 이의 지방세포 분화 억제 효능을 규명하였다. 본 연구에서는 생코끼리마늘(Raw Elephant Rarlic, REG) 및 흑코끼리마늘(Steam Elephant Garlic, SEG) 추출물이 지방세포의 분화 및 지질축적을 억제한다는 것을 보여주었다. 주목할 만한 점은, REG의 경우 지방세포에 독성을 나타낸 반면, SEG는 독성을 나타내지 않았다는 점이다. 추가적으로, SEG는 PPARγ, C/EBPα, Adiponectin, Ap2 및 LPL와 같은 지방세포 분화 관련 유전자의 발현을 통계적으로 유의하게 억제하였다. 본 연구는 두 종류의 코끼리 마늘이 3T3-L1지방세포의 분화를 억제함을 보고한 최초의 연구이다.

Keywords

References

  1. Kwon OC, Woo KS, Kim TM, Kim DJ, 2006. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 331-336.
  2. Kang MJ, Lee SJ, Shin JH, Kang SK, Kim JG, Sung NJ. 2008. Effect of garlic with different processing on lipid metabolism in 1% cholesterol fed rats. J. Korean Soc. Food Sci. Nutr. 37: 162-169. https://doi.org/10.3746/jkfn.2008.37.2.162
  3. Elkayam A, Mirelman D, Peleg E, Wilchek M, Miron T, Rabinkov A, et al. 2003. The effects of allicin on weight in fructoseinduced hyperinsulinemic, hyperlipidemic, hypertensive rats. Am. J. Hypertens. 16: 1053-1056. https://doi.org/10.1016/j.amjhyper.2003.07.011
  4. Kim D, Kim KH, Yook HS. 2015. Analysis of active components of giant black garlic. J. Korean Soc. Food Sci. Nutr. 44: 1672-1681. https://doi.org/10.3746/jkfn.2015.44.11.1672
  5. Byun PH, Kim WJ, Yoon SK. 2001. Changes of functional properties of garlic extracts during storage. Korean J. Food Sci. Technol. 33: 301-306.
  6. Kang JR, Lee SJ, Hwang CR, Kim IS, Sung NJ. 2013. Effect of black garlic and Gaeddongssuk (Artemisia annua L.) extracts on the lipid profile and hepatic antioxidant enzyme activities of exercised rats. J. Korean Soc. Food Sci. Nutrition 42: 869-876. https://doi.org/10.3746/jkfn.2013.42.6.869
  7. Youn CK, Kim KH, Ahn KS, Jaeng JH, Park YU, Kwon YH, et al. 2015. Effect of planting density on yield and growth characteristics of elephant garlic. Korean J. Plant Resour. 28: 541-545. https://doi.org/10.7732/kjpr.2015.28.4.541
  8. Lu X, Ross CF, Powers JR, Aston DE, Rasco BA. 2011. Determination of total phenolic content and antioxidant activity of garlic (Allium sativum) and elephant garlic (Allium ampeloprasum) by attenuated total reflectance-fourier transformed infrared spectroscopy. J. Agric. Food Chem. 59: 5215-5221. https://doi.org/10.1021/jf201254f
  9. Rahimi-Madiseh M, Heidarian E, Kheiri S, Rafieian-Kopaei M. 2017. Effect of hydroalcoholic Allium ampeloprasum extract on oxidative stress, diabetes mellitus and dyslipidemia in alloxaninduced diabetic rats. Biomed. pharmacother. 86: 363-367. https://doi.org/10.1016/j.biopha.2016.12.028
  10. Hughes BG, Lawson LD. 1991. Antimicrobial effects of Allium sativum L. (garlic), Allium ampeloprasum L. (elephant garlic), and Allium cepa L. (onion), garlic compounds and commercial garlic supplement products. Phytother. Res. 5: 154-158. https://doi.org/10.1002/ptr.2650050403
  11. Ham JR, Lee H-I, Choi R-Y, Sim M-O, Choi M-S, Kwon E-Y, et al. 2016. Anti-obesity and anti-hepatosteatosis effects of dietary scopoletin in high-fat diet fed mice. J. Funct. Foods. 25: 433-446. https://doi.org/10.1016/j.jff.2016.06.026
  12. Lee SG, Kim JS, Min K, Kwon TK, Nam JO. 2018. Hispidulin inhibits adipogenesis in 3T3-L1 adipocytes through PPAR$\gamma$ pathway. Chem. Biol. Interact. 293: 89-93. https://doi.org/10.1016/j.cbi.2018.07.027
  13. Lee SG, Kwon TK, Nam J-O. 2017. Silibinin inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Microbiol. Biotechnol. Lett. 45: 27-34. https://doi.org/10.4014/mbl.1610.10005
  14. Lee JA, Ahn E-K, Hong SS, Oh JS. 2012. Anti-obesity effect of ethyl acetate extracts from Agrimonia pilosa Ledeb. in 3T3-L1 preadipocytes (62.3). J. Immunol. 188: 62.63-62.63.