Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparative Genomics Study of Candidatus Carsonella Ruddii; an Endosymbiont of Economically Important Psyllids

  • Mondal, Shakhinur Islam (Genetic Engineering and Biotechnology Department, Shahjalal University of Science and Technology) ;
  • Akter, Elma (Genetic Engineering and Biotechnology Department, Shahjalal University of Science and Technology) ;
  • Akter, Arzuba (Biochemistry and Molecular Biology Department, Shahjalal University of Science and Technology) ;
  • Khan, Md Tahsin (Genetic Engineering and Biotechnology Department, Shahjalal University of Science and Technology) ;
  • Jewel, Nurnabi Azad (Genetic Engineering and Biotechnology Department, Shahjalal University of Science and Technology)
  • Received : 2019.12.03
  • Accepted : 2020.04.17
  • Published : 2020.09.28
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Abstract

Candidatus Carsonella ruddii is an endosymbiont that resides in specialized cells within the body cavity of plant sap-feeding insects called psyllids. The establishment of symbiotic associations is considered one of the key factors for the evolutionary success of psyllids, as it may have helped them adapt to imbalanced food resources like plant sap. Although C. ruddii is defined as a psyllid primary symbiont, the genes for some essential amino acid pathways are absent. Complete genome sequences of several C. ruddii strains have been published. However, in-depth intra-species comparison of C. ruddii strains has not yet been done. This study therefore aimed to perform a comparative genome analysis of six C. ruddii strains, allowing the interrogation of phylogenetic group, functional category of genes, and biosynthetic pathway analysis. Accordingly, overall genome size, number of genes, and GC content of C. ruddii strains were reduced. Phylogenetic analysis based on the whole genome proteomes of 30 related bacterial strains revealed that the six C. ruddii strains form a cluster in same clade. Biosynthetic pathway analysis showed that complete sets of genes for biosynthesis of essential amino acids, except tryptophan, are absent in six C. ruddii strains. All genes for tryptophan biosynthesis are present in three C. ruddii strains (BC, BT, and YCCR). It is likely that the host may depend on a secondary symbiont to complement its deficient diet. Overall, it is therefore possible that C. ruddii is being driven to extinction and replacement by new symbionts.

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References

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