Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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Stable Isotope Labeling of Proteins in Mammalian Cells

  • Lee, KyungRyun (Department of Chemistry, Seoul National University) ;
  • Lee, Jung Ho (Department of Chemistry, Seoul National University)
  • Received : 2020.09.10
  • Accepted : 2020.09.18
  • Published : 2020.09.20
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Abstract

Stable isotope enrichment in proteins is necessary for high-resolution nuclear magnetic resonance (NMR) experiments. Although methods for 13C, 15N and 2H-enrichment in prokaryotic cells are well established, full processing and correct folding of complex protein systems require higher organisms as the expression host. In the present study, we review recent efforts to enrich stable isotopes in mammalian cells for protein NMR studies.

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References

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