Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Toxicity of doxycycline, a broad-spectrum semi-synthetic antibiotic, in Pacific whiteleg shrimp (Litopenaeus vannamei)

흰다리새우(Litopenaeus vannamei)에서 광범위 반합성 항생제 doxycycline의 독성연구

  • Lee, Chae Won (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Bae, Jun Sung (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Yang, Chan Yeong (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Jeoung, Eun Ha (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Lee, Ji-Hoon (Aquatic Animal Disease Control Center, National Institute of Fisheries Science (NIFS)) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University)
  • 이채원 (군산대학교 해양과학대학 수산생명의학과) ;
  • 배준성 (군산대학교 해양과학대학 수산생명의학과) ;
  • 양찬영 (군산대학교 해양과학대학 수산생명의학과) ;
  • 정은하 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이지훈 (국립수산과학원 수산방역과) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2020.05.25
  • Accepted : 2020.06.17
  • Published : 2020.06.30
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Abstract

Doxycycline is a semi-synthetic broad-spectrum antibiotic, and it has been used to get rid of bacteria in animals and humans. The use of antibiotics has greatly contributed to the aquaculture production although its misuse sometimes presents public health problems. This study was performed to investigate the toxic effects of doxycycline on whiteleg shrimp (Litopenaeus vannamei) administered for possible infection treatments. The shrimp were allocated into four groups and doxycycline was fed three times a day for 7 days at 0, 20, 50 and 100 mg/kg to each group. After 24 hr following the 7-day treatment, hemolymph and hepatopancreas were used for blood and biochemical analysis: Total hemocyte counts, Total protein, Total cholesterol, Gluscose, Glutamic pyruvic transaminase, Glutamic oxaloacetic transaminase, Glutathione peroxidase, Superoxide dismutase, Glutathione-s-transferases, Total antioxidant capacity colorimetric and Acid phosphatase. In addition, histopathological examination was performed on the hepatopancreas and muscle. It was observed that body weight gain was significantly retarded in 100 mg/kg doxycycline group. Doxycycline was found to induce biochemical or functional disorders at 100 mg/kg as observed many of the blood and biochemical parameters were significantly reduced. In conclusion, it was judged that there will be no major toxicity problems with doxycycline when used for shrimp aquaculture at regular doses.

Keywords

References

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