Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Therapeutic study of myxosporean emaciation disease of olive flounder (Paralichthys olivaceus) in Jeju using toltrazuril

톨트라주릴(Toltrazuril)을 이용한 제주도 넙치(Paralichthys olivaceus)의 점액포자충성 여윔증에 대한 치료법 연구

  • Kang, Mi-Rae (Department of Marine Life Sciences, Jeju National University) ;
  • Kim, Ye-Ji (Department of Marine Life Sciences, Jeju National University) ;
  • Jun, Lyu Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Kim, Seung Min (Seaweed Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Sung-Hyun (Fishcare laboratory) ;
  • Han, Sori (Fishcare laboratory) ;
  • Jeong, Joon Bum (Department of Marine Life Sciences, Jeju National University)
  • 강미래 (제주대학교 해양생명과학과) ;
  • 김예지 (제주대학교 해양생명과학과) ;
  • 전려진 (제주대학교 해양생명과학과) ;
  • 김승민 (국립수산과학원 해조류연구센터) ;
  • 김성현 (피쉬케어연구소) ;
  • 한소리 (피쉬케어연구소) ;
  • 정준범 (제주대학교 해양생명과학과)
  • Received : 2020.06.01
  • Accepted : 2020.06.17
  • Published : 2020.06.30
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Abstract

In order to search candidates for the treatment of myxosporean emaciation disease of olive flounder, Paralichthys olivaceus, in vitro and in vivo experiments were conducted using products such as Coxi-stop, Coxiclin, and BLEAN80. In the case of Coxi-stop, whose major component is toltrazuril, in vitro experiment using BF-2 cells showed a tendency of reducing the activity of myxosporea and did not exhibit cytotoxicity such as cell lysis. In the in vivo activity measurements, the experimental group immersed with Coxi-stop showed a lower cumulative mortality rate than the control group, this result is similar to the previous report that toltrazuril has a therapeutic effect on parasitic fish disease. This study suggests that toltrazuril is a potential candidate for the treatment of emaciation disease of olive flounder.

본 연구에서는 점액포자충에 의한 넙치의 여윔증 치료 후보물질을 탐색하기 위하여, Coxi-stop, Coxiclin 및 BLEAN80 등의 제품을 사용하여 in vitro 및 in vivo 실험을 실시하였다. 톨트라주릴이 주성분인 Coxi-stop의 경우, BF-2 cell을 사용한 in vitro 실험에서 점액포자충의 활성을 감소시키는 결과를 보였고, cell lysis와 같은 세포 독성 현상이 나타나지 않았다. In vivo 실험에서 Coxi-stop을 사용하여 약욕한 실험군은 대조군에 비하여 누적폐사율이 낮게 나타났으며, 이것은 톨트라주릴이 어류 기생충성 질병에 치료 효과가 있다는 기존의 보고와 유사한 결과이다. 본 연구에서는 톨트라주릴이 넙치의 여윔증 치료제로서 가능성이 있는 후보 물질이라는 것을 제시한다.

Keywords

References

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