Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Protective effects of four types of Taraxaci Herba water extract on carbon tetrachloride-induced acute liver injuries in mice

사염화탄소 유도성 급성 간 손상 모델에서 포공영(蒲公英) 열수 추출물 4종의 간 보호 효능 연구

  • Choi, Beom-Rak (Nutracore Co., Ltd) ;
  • Cho, Il Je (College of Korean Medicine, Daegu Haany University) ;
  • Jung, Su-Jin (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Kim, Jae Kwang (College of Korean Medicine, Daegu Haany University) ;
  • Lee, Dae Geon (College of Korean Medicine, Daegu Haany University) ;
  • Ku, Sae Kwang (College of Korean Medicine, Daegu Haany University) ;
  • Park, Ki-Moon (Department of Foodscience and Biotechnology, Sungkyunkwan University)
  • 최범락 (뉴트라코어) ;
  • 조일제 (대구한의대학교 한의과대학) ;
  • 정수진 (전북대학교병원 의생명연구센터) ;
  • 김재광 (대구한의대학교 한의과대학) ;
  • 이대건 (대구한의대학교 한의과대학) ;
  • 구세광 (대구한의대학교 한의과대학) ;
  • 박기문 (성균관대학교 식품생명공학과)
  • Received : 2019.07.02
  • Accepted : 2019.09.17
  • Published : 2020.02.28
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Abstract

Objectives : Present study investigated hepatoprotective effects of four types of Taraxaci Herba water extract (TL-F, Taraxaci Herba leaf originated from foreign country; TR-F, Taraxaci Herba root originated from foreign country; TL-K, Taraxaci Herba leaf originated from Korea; TR-K, Taraxaci Herba root originated from Korea) on carbon tetrachloride (CCl4)-induced acute liver injury. Methods : Mice were administered orally with 200 mg/kg of TL-F, TR-F, TL-K, or TR-K for seven days, and intraperitoneally injected with 0.5 mL/kg of CCl4 1 h after last Taraxaci Herba treatment. Silymarin (100 mg/kg) was used as a positive drug. Body weight gain, relative liver weight, serum biochemistry, histopathological and immunohistochemical analyses, and hepatic antioxidant capacity were determined to investigate the hepatoprotective effect of four extracts. Results : Administration of four types of Taraxaci Herba extract increased body weight gain, and decreased relative liver weight in CCl4-injected mice. As compared to CCl4 group, TL-F and TR-F significantly decreased serum aspartate aminotransferase activity, while four extracts reduced CCl4-induced alanine aminotransferase activity. In addition, TL-F and TR-F significantly decreased the numbers of degenerated hepatocytes, infiltrated inflammatory cells, cleaved caspase-3 positive cells, and cleaved PARP positive cells in hepatic tissues. Moreover, TL-F, TR-F, and TR-K administration reduced the lipid peroxidation and nitrotyrosine, and increased glutathione, superoxide dismutase, and catalase activities in hepatic tissues. There were no statistical differences between TL-F- and silymarin-treated group. Conclusion : Of four extracts tested, present results suggest that TL-F is the most favorable candidate against CCl4-induced acute liver injury through enhancing antioxidant activity.

Keywords

References

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