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http://dx.doi.org/10.14374/HFS.2020.28.1.1

Protective effects of four types of Taraxaci Herba water extract on carbon tetrachloride-induced acute liver injuries in mice  

Choi, Beom-Rak (Nutracore Co., Ltd)
Cho, Il Je (College of Korean Medicine, Daegu Haany University)
Jung, Su-Jin (Biomedical Research Institute of Chonbuk National University Hospital)
Kim, Jae Kwang (College of Korean Medicine, Daegu Haany University)
Lee, Dae Geon (College of Korean Medicine, Daegu Haany University)
Ku, Sae Kwang (College of Korean Medicine, Daegu Haany University)
Park, Ki-Moon (Department of Foodscience and Biotechnology, Sungkyunkwan University)
Publication Information
Herbal Formula Science / v.28, no.1, 2020 , pp. 1-13 More about this Journal
Abstract
Objectives : Present study investigated hepatoprotective effects of four types of Taraxaci Herba water extract (TL-F, Taraxaci Herba leaf originated from foreign country; TR-F, Taraxaci Herba root originated from foreign country; TL-K, Taraxaci Herba leaf originated from Korea; TR-K, Taraxaci Herba root originated from Korea) on carbon tetrachloride (CCl4)-induced acute liver injury. Methods : Mice were administered orally with 200 mg/kg of TL-F, TR-F, TL-K, or TR-K for seven days, and intraperitoneally injected with 0.5 mL/kg of CCl4 1 h after last Taraxaci Herba treatment. Silymarin (100 mg/kg) was used as a positive drug. Body weight gain, relative liver weight, serum biochemistry, histopathological and immunohistochemical analyses, and hepatic antioxidant capacity were determined to investigate the hepatoprotective effect of four extracts. Results : Administration of four types of Taraxaci Herba extract increased body weight gain, and decreased relative liver weight in CCl4-injected mice. As compared to CCl4 group, TL-F and TR-F significantly decreased serum aspartate aminotransferase activity, while four extracts reduced CCl4-induced alanine aminotransferase activity. In addition, TL-F and TR-F significantly decreased the numbers of degenerated hepatocytes, infiltrated inflammatory cells, cleaved caspase-3 positive cells, and cleaved PARP positive cells in hepatic tissues. Moreover, TL-F, TR-F, and TR-K administration reduced the lipid peroxidation and nitrotyrosine, and increased glutathione, superoxide dismutase, and catalase activities in hepatic tissues. There were no statistical differences between TL-F- and silymarin-treated group. Conclusion : Of four extracts tested, present results suggest that TL-F is the most favorable candidate against CCl4-induced acute liver injury through enhancing antioxidant activity.
Keywords
Acute liver injury; Antioxidant activity; Carbon tetrachloride ($CCl_4$); Oxidative stress; Taraxaci Herba water extract;
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