Korean Chemical Engineering Research

  • 제58권2호
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  • Pages.286-292
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  • 2020
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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회전각도를 이용한 알부민 농도 측정용 3차원 종이 칩

Angular-based Measurement for Quantitative assay of Albumin in three-dimensional Paper-based analytical Device

  • 김동호 (충남대학교 응용화학공학과) ;
  • 정성근 (충남대학교 응용화학공학과) ;
  • 이창수 (충남대학교 응용화학공학과)
  • Kim, Dong-Ho (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Jeong, Seong-Geun (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Lee, Chang-Soo (Department of Chemical Engineering and Applied Chemistry, Chungnam National University)
  • 투고 : 2020.01.19
  • 심사 : 2020.02.14
  • 발행 : 2020.05.01
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초록

본 연구에서는 별도의 이미지 분석 장비를 사용하지 않고 정량적으로 알부민의 농도를 측정할 수 있는 회전각도를 이용한 3차원 종이 칩(3D-PADs)를 제시한다. 변색된 구간의 회전각도를 측정하는 간단한 방법으로 검출을 시연하였다. 3D-PADs는 왁스 프린터를 이용한 인쇄와 라미네이팅 과정을 거쳐 빠르게 제작할 수 있다. 3D-PADs는 샘플의 알부민을 검출하기 위하여 citrate buffer와 tetrabromophenol blue를 흡수시켰다. 3D-PAD의 흡수패드에 샘플 용액을 흡수시키면, 샘플 용액은 형성된 유로를 통하여 수직 및 수평 흐름을 통해 분석 구간으로 흐른다. 변색된 구간의 회전각도는 특정한 알부민의 농도를 나타내며, 알부민 측정의 신뢰할 수 있는 값임을 확인할 수 있었다.

This study presents an angular-based measurement on three-dimensional paper-based analytical devices (3D-PADs) for quantitative detection of albumin without using an image analyzer. We demonstrate a simple quantitative and straightforward approach based on the angle of the discolored area as detection criteria. 3D-PADs are rapidly fabricated by the wax-printing and laminating process. The 3D-PADs are treated with citrate buffer and tetrabromophenol blue to react with albumin in a sample solution. Dropping sample solution into sample pad in the 3D-PAD, fluid flows toward the assay zone laterally and vertically by capillary action. We find that the change of angle of the discolored area correctly reflects the concentration of albumin and is reliable determinant for the measurement of the albumin concentration. It is the first demonstration of angular-based detection as a simple, inexpensive, and equipment-free approach for point-of-care diagnosis.

키워드

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