Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton

  • Li, Ling (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University) ;
  • Zhuang, Pingping (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University) ;
  • Cheng, Ziqiang (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University) ;
  • Yang, Jie (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University) ;
  • Bi, Jianmin (China Animal Husbandry Industry Co. Ltd.) ;
  • Wang, Guihua (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University)
  • Received : 2020.01.10
  • Accepted : 2020.03.12
  • Published : 2020.05.31
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Abstract

Background: Coinfection with avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV) is common in chickens, and the molecular mechanism of the synergistic pathogenic effects of the coinfection is not clear. Exosomes have been identified as new players in the pathogenesis of retroviruses. The different functions of exosomes depend on their cargo components. Objectives: The aim of this study was to investigate the function of co-regulation differentially expressed proteins in exosomes on coinfection of ALV-J and REV. Methods: Here, viral replication in CEF cells infected with ALV-J, REV or both was detected by immunofluorescence microscopy. Then, we analyzed the exosomes isolated from supernatants of chicken embryo fibroblast (CEF) cells single infected and coinfected with ALV-J and REV by mass spectrometry. KEGG pathway enrichment analyzed the co-regulation differentially expressed proteins in exosomes. Next, we silenced and overexpressed tripartite motif containing 62 (TRIM62) to evaluate the effects of TRIM62 on viral replication and the expression levels of NCK-association proteins 1 (NCKAP1) and actin-related 2/3 complex subunit 5 (ARPC5) determined by quantitative reverse transcription polymerase chain reaction. Results: The results showed that coinfection of ALV-J and REV promoted the replication of each other. Thirty proteins, including TRIM62, NCK-association proteins 1 (NCKAP1, also known as Nap125), and Arp2/3-5, ARPC5, were identified. NCKAP1 and ARPC5 were involved in the actin cytoskeleton pathway. TRIM62 negatively regulated viral replication and that the inhibition of REV was more significant than that on ALV-J in CEF cells coinfected with TRIM62. In addition, TRIM62 decreased the expression of NCKAP1 and increased the expression of ARPC5 in coinfected CEF cells. Conclusions: Collectively, our results indicated that coinfection with ALV-J and REV competitively promoted each other's replication, the actin cytoskeleton played an important role in the coinfection mechanism, and TRIM62 regulated the actin cytoskeleton.

Keywords

Acknowledgement

This work was supported by the Natural Science Foundation of Shandong Provincial (ZR2017MC011) and the National Natural Science Foundation of China (31772703).

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