Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Rust Disease Caused by Puccinia phragmitis on Rumex japonicus in Korea

Puccinia phragmitis에 의한 참소리쟁이 잎녹병 발생 보고

  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Park, Ji-Won (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kang, In-Kyu (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • 이승열 (경북대학교 농업생명과학대학) ;
  • 박지원 (경북대학교 농업생명과학대학) ;
  • 강인규 (경북대학교 농업생명과학대학) ;
  • 정희영 (경북대학교 농업생명과학대학)
  • Received : 2020.03.16
  • Accepted : 2020.03.26
  • Published : 2020.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Red spots symptoms were observed on the leaves of Rumex japonicus Houtt. in Goesan, Korea, in May 2019. Diseased leaf showing red spot and white-colored sori were found behind of diseased leaves. Aeciospores were confirmed and observed using a stereomicroscope, light microscope, and scanning electron microscope. They were globose or ovoid and measured 19.0-24.4×19.0-24.5 ㎛, with verrucae ornamentation. For the phylogenetic analysis, partial of large subunit rDNA region and cytochrome c oxidase subunit III were sequenced. As a result, the isolated fungus species showed high similarities with Puccinia prizeliana. Based on the previous report, morphological characters, phylogenetic analysis, it was confirmed as Puccinia phragmitis which have not been reported in Korea, and we firstly report this fungal agent on R. japonicus as P. phragmitis in Korea.

2019년 5월, 잎 표면에서 적색 반점이 관찰되는 참소리쟁이를 충북 괴산군 인근 야산에서 채집하였다. 관찰된 병반의 앞면은 적색 및 황색의 병반이 관찰되었고, 뒷면에는 백색의 돌기가 관찰되었다. 다양한 현미경으로 자세하게 관찰한 결과, 돌기는 녹포자기로 확인되었으며, 많은 녹포자가 확인되었다. 녹포자는 구형 또는 난형이었고 19.0-24.4×19.0-24.5 ㎛의 크기를 가지면서 표면은 무사마귀형으로 관찰되었다. 계통학적 유연관계 분석을 위하여, large subunit rDNA 영역 및 cytochrome c oxidase subunit 3 (CO3) 유전자 영역을 이용하여 계통학적 유연관계를 분석한 결과, 참소리쟁이에서 관찰된 녹병균은 Puccinia pritzliana와 매우 근연한 관계를 가지고 있었다. 관찰된 기주, 포자세대, 형태 및 계통학적 유연관계 결과를 종합해 볼 때, 분석된 녹병균은 국내 미보고 녹병균인 P. phragmitis로 확인하였으며, 위 병원균에 의해 발생한 녹병을 참소리쟁이 잎녹병으로 제안하고자 한다.

Keywords

References

  1. Ahn, J.-Y., Im, L.-R., Kim, J.-H., Park, J.-H., Kim, D.-K. and Lee, Y.-M. 2009. Effects of Rumecis radix water extract on development of atopic dermatitis in BALB/c mice. Korean J. Pharmacogn. 40: 218-223.
  2. Bae, G. H. 2000. The Medicinal Plant of Korea. Kyohasa, Seoul, Korea. 231 pp.
  3. Beenken, L., Zoller, S. and Berndt, R. 2012. Rust fungi on Annonaceae II: the genus Dasyspora Berk. & M.A. Curtis. Mycologia 104: 659-681. https://doi.org/10.3852/11-068
  4. Brandbyge, J. 1989. Notes on the genus Ruprechtia (Polygonaceae). Nord. J. Bot. 9: 57-61. https://doi.org/10.1111/j.1756-1051.1989.tb00985.x
  5. Farr, D. F. and Rossman, A. Y. 2020. Fungal databases. URL https://nt.ars-grin.gov/fungaldatabases/index.cfm [15 March 2020].
  6. Inman, R. E. 1970. Observationson the biology of Rumex rust Uromyces rumicis (Schum.) Wint. Bot. Gaz. 131: 234-241. https://doi.org/10.1086/336537
  7. Kim, C. S., Lee, I. Y., Lee J. R. and Oh, Y. J. 2015. Illustrated Weed Flora in Orchard. National Institute of Agricultural Sciences, Wanju, Korea. 455 pp.
  8. Lamb, I. M. 1935. The intiation of the dikaryophase in Puccinia phragmitis (Schum.) Korn. Ann. Bot. 49: 403-438. https://doi.org/10.1093/oxfordjournals.aob.a090515
  9. Lee, J. T., Kim, M. H. and Park, K. S. 1983. On the fungal diseases of medicinal plant in Korea. Res. Rev. Kyungpook Natl. Univ. 36: 553-561.
  10. Lee, T. S. 2001. Pucciniaceae of Korea (Uredinales). National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Korea. 81 pp.
  11. Li, Y.-P., Takamiyagi, A., Ramzi, S. T. and Nonaka, S. 2000. Inhibitory effect of Rumex japonicus Houtt on the porphyrin photooxidative reaction. J. Dermatol. 27: 761-768. https://doi.org/10.1111/j.1346-8138.2000.tb02278.x
  12. Marin-Felix, Y., Groenewald, J. Z., Cai, L., Chen, Q., Marincowitz, S., Barnes, I. et al. 2017. Genera of phytopathogenic fungi: GOPHY 1. Stud. Mycol. 86: 99-216. https://doi.org/10.1016/j.simyco.2017.04.002
  13. Moncalvo, J.-M., Wang, H.-H. and Hseu, R.-S. 1995. Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia 87: 223-238. https://doi.org/10.2307/3760908
  14. Namba, S., Kato, S., Iwanami, S., Oyaizu, H., Shiozawa, H. and Tsuchizaki, T. 1993. Detection and differentiation of plantpathogenic mycoplasmalike organisms using polymerase chain reaction. Phytopathology 83: 786-791. https://doi.org/10.1094/Phyto-83-786
  15. National Institute of Biological Resources. 2018. National Species List of Korea. I. Plant, Fungi, Algae, Progaryotes. Designzip, Seoul, Korea. 988 pp.
  16. Park, Y.-K. 2003. Studies on the anti-oxidative effects of Rumexis Radix. Korean J. Herbol. 18: 269-277.
  17. Parmelee, J. A. 1963. Puccinia phragmitis in Canada. Mycologia 55: 133-141. https://doi.org/10.2307/3756284
  18. Vialle, A., Feau, N., Allaire, M., Didukh, M., Martin, F., Moncalvo, J.-M. et al. 2009. Evaluation of mitochondrial genes as DNA barcode for Basidiomycota. Mol. Ecol. Resour. 9(Suppl. 1): 99-113. https://doi.org/10.1111/j.1755-0998.2009.02637.x
  19. Vilgalys, R. and Hester, M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 172: 4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  20. Yildirim, A., Mavi, A. and Kara, A. A. 2001. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J. Agric. Food Chem. 49: 4083-4089. https://doi.org/10.1021/jf0103572