Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Stress Hormone Cortisol Damages the Skin Barrier by Regulating Tight Junctions

밀착연접 조절을 통한 스트레스 호르몬 코티졸의 피부장벽 손상 연구

  • Lee, Sung Hoon (Basic Research & Innovation Division, Amorepacific Corporation R&D Center) ;
  • Son, Eui Dong (Basic Research & Innovation Division, Amorepacific Corporation R&D Center) ;
  • Choi, Eun-Jeong (Basic Research & Innovation Division, Amorepacific Corporation R&D Center) ;
  • Park, Won-Seok (Basic Research & Innovation Division, Amorepacific Corporation R&D Center) ;
  • Kim, Hyoung-June (Basic Research & Innovation Division, Amorepacific Corporation R&D Center)
  • 이성훈 ((주)아모레퍼시픽 기술연구원) ;
  • 손의동 ((주)아모레퍼시픽 기술연구원) ;
  • 최은정 ((주)아모레퍼시픽 기술연구원) ;
  • 박원석 ((주)아모레퍼시픽 기술연구원) ;
  • 김형준 ((주)아모레퍼시픽 기술연구원)
  • Received : 2020.03.12
  • Accepted : 2020.03.30
  • Published : 2020.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Psychological stress can affect the physiological condition of the skin and cause various cutaneous disorders. The stress hormone cortisol is secreted by various skin cells such as fibroblasts, keratinocytes, and melanocytes. Tight junctions (TJs) are cell-cell junctions that form a barrier in the stratum granulosum of mammalian skin. TJs can also affect other skin barriers and are affected by chemical, microbial, or immunological barriers. Stress can cause damage to the skin barrier. Interestingly, to our knowledge, there has not been any research demonstrating the involvement of TJs in this process. In this study, cortisol was used to treat keratinocytes to determine its role in regulating TJs. We found that cortisol damaged skin barrier function by regulating the gene expression and structure of TJ components. Cortisol also inhibited the development of the granular layer in a skin equivalent model. These results suggest that cortisol affects the skin barrier function by the regulation of TJs.

심리적 스트레스는 피부의 생리적 상태에 영향을 미치고 다양한 피부 질환을 일으킬 수 있다. 스트레스 호르몬인 코티솔은 섬유질, 케라틴세포, 멜라노사이트와 같은 다양한 피부세포에 의해 분비된다. 밀착연접(tight junction, TJ) 은 포유류 피부의 과립증에서 장벽을 형성하는 세포 접합부위이다. TJ은 다른 피부 장벽기능에도 영향을 미칠 수 있으며 화학, 미생물 또는 면역학적 피부장벽에게 영향을 받는다. 스트레스로 인한 피부 장벽 손상에 관한 보고는 있지만 사람피부에서 코티솔이 TJ을 조절한다는 보고는 없다. 스트레스 호르몬 코티솔이 TJ을 조절하는 기능을 확인하기 위해 각질형성세포에 코티솔을 처리하였다. 코티솔은 TJ 구성 성분의 유전자 발현과 구조를 조절하여 피부 장벽 기능을 손상시켰다. 또한 코티솔은 인공피부 모델에서 과립층 형성을 억제하였다. 이러한 실험결과를 통해 스트레스 호르몬 코티솔이 TJ를 조절함으로써 피부 장벽 기능에 손상을 일으키는 것을 확인할 수 있었다.

Keywords

References

  1. E. Orion and R. Wolf, Psychological stress and epidermal barrier function, Clin. Dermatol., 30(3), 280 (2012). https://doi.org/10.1016/j.clindermatol.2011.08.014
  2. R. M. Sapolsky, L. M. Romero, and A. U. Munck, How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions, Endocr. Rev., 21(1), 55 (2000). https://doi.org/10.1210/edrv.21.1.0389
  3. J. Zhou, J. A. Cidlowski, The human glucocorticoid receptor: one gene, multiple proteins and diverse responses, Steroids, 70(5-7), 407 (2005). https://doi.org/10.1016/j.steroids.2005.02.006
  4. M. D enda, T. T suchiya, P . M. Elias, a nd K. R. Feingold, Stress alters cutaneous permeability barrier homeostasis, Am. J. Physiol. Regul. Integr. Comp. Physiol., 278(2), R367 (2000). https://doi.org/10.1152/ajpregu.2000.278.2.R367
  5. A. Garg, M. M. Chren, L. P. Sands, M. S. Matsui, K. D. Marenus, K. R. Feingold, and P. M. Elias, Psychological stress perturbs epidermal permeability barrier homeostasis: implications for the pathogenesis of stress-associated skin disorders, Arch Dermatol, 137(1), 53 (2001).
  6. A. T. Slominski, P. R. Manna, and R. C. Tuckey, Cutaneous glucocorticosteroidogenesis: securing local homeostasis and the skin integrity, Exp. Dermatol., 23(6), 369 (2014). https://doi.org/10.1111/exd.12376
  7. G. Nikolakis and C. C. Zouboulis, Skin and glucocorticoids: effects of local skin glucocorticoid impairment on skin homeostasis, Exp. Dermatol., 23(11), 807 (2014). https://doi.org/10.1111/exd.12519
  8. N. Cirillo and S. S. Prime, Keratinocytes synthesize and activate cortisol, J Cell Biochem, 112(6), 1499 (2011). https://doi.org/10.1002/jcb.23081
  9. A. Slominski, B. Zbytek, A. Szczesniewski, I. Semak, J. Kaminski, T. Sweatman, and J. Wortsman, CRH stimulation of corticosteroids production in melanocytes is mediated by ACTH, Am. J. Physiol. Endocrinol. Metab., 288(4), E701 (2005). https://doi.org/10.1152/ajpendo.00519.2004
  10. M. Terao, H. Murota, A. Kimura, A. Kato, A. Ishikawa, K. Igawa, E. Miyoshi, and I. Katayama, 11beta- Hydroxysteroid dehydrogenase-1 is a novel regulator of skin homeostasis and a candidate target for promoting tissue repair, PLoS One, 6(9), e25039 (2011). https://doi.org/10.1371/journal.pone.0025039
  11. A. Slominski, J. Wortsman, R. C. Tuckey, and R. Paus, Differential expression of HPA axis homolog in the skin, Mol. Cell. Endocrinol., 265-266, 143 (2007). https://doi.org/10.1016/j.mce.2006.12.012
  12. C. Skobowiat, J. C. Dowdy, R. M. Sayre, R. C. Tuckey, and A. Slominski, Cutaneous hypothalamicpituitary- adrenal axis homolog: regulation by ultraviolet radiation, Am. J. Physiol. Endocrinol. Metab., 301(3), E484 (2011). https://doi.org/10.1152/ajpendo.00217.2011
  13. C. Skobowiat, R. Nejati, L. Lu, R. W. Williams, and A. T. Slominski, Genetic variation of the cutaneous HPA axis: an analysis of UVB-induced differential responses, Gene, 530(1), 1 (2013). https://doi.org/10.1016/j.gene.2013.08.035
  14. C. Skobowiat, R. M. Sayre, J. C. Dowdy, and A. T. Slominski, Ultraviolet radiation regulates cortisol activity in a waveband-dependent manner in human skin ex vivo, Br. J. Dermatol., 168(3), 595 (2013). https://doi.org/10.1111/bjd.12096
  15. M. Furuse, M. Hata, K. Furuse, Y. Yoshida, A. Haratake, Y. Sugitani, T. Noda, A. Kubo, and S. Tsukita, Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice, J. Cell. Biol., 156(6), 1099 (2002). https://doi.org/10.1083/jcb.200110122
  16. T. Yuki, A. Hachiya, A. Kusaka, P. Sriwiriyanont, M. O. Visscher, K. Morita, M. Muto, Y. Miyachi, Y. Sugiyama, and S. Inoue, Characterization of tight junctions and their disruption by UVB in human epidermis and cultured keratinocytes, J. Invest. Dermatol., 131(3), 744 (2011). https://doi.org/10.1038/jid.2010.385
  17. J. M. Brandner, Importance of tight junctions in relation to skin barrier function, Curr. Probl. Dermatol., 49, 27 (2016). https://doi.org/10.1159/000441541
  18. Y. Zong, S. Zhu, S. Zhang, G. Zheng, J. W. Wiley, and S. Hong, Chronic stress and intestinal permeability: Lubiprostone regulates glucocorticoid receptor mediated changes in colon epithelial tight junction proteins, barrier function, and visceral pain in the rodent and human, Neurogastroenterol. Motil., 31(2), e13477 (2018). https://doi.org/10.1111/nmo.13477
  19. G. Zheng, S. P. Wu, Y. Hu, D. E. Smith, J. W. Wiley, and S. Hong, Corticosterone mediates stress-related increased intestinal permeability in aregion-specific manner, Neurogastroenterol. Motil., 25(2), e127 (2013). https://doi.org/10.1111/nmo.12066
  20. G. Zheng, G. Victor Fon, W. Meixner, A. Creekmore, Y. Zong, K. Dame M, J. Colacino, P. H. Dedhia, S. Hong, and J. W. Wiley, Chronic stress and intestinal barrier dys function: Glucocorticoid receptor and transcription repressor HES1 regulate tight junction protein Claudin-1 promoter, Sci Rep, 7(1), 4502 (2017). https://doi.org/10.1038/s41598-017-04755-w
  21. R. Sultana, A. J. Mcbain, and C. A. O'neill, Straindependent augmentation of tight-junction barrier functionin human primary epidermal keratinocytes by Lactobacillus and Bifidobacterium lysates, Appl. Environ. Microbiol., 79(16), 4887 (2013). https://doi.org/10.1128/AEM.00982-13
  22. I. H. Kuo, A. Carpenter-Mendini, T. Yoshida, L. Y. Mcgirt, A. I. Ivanov, K. C. Barnes, R. L. Gallo, A. W. Borkowski, K. Yamasaki, D. Y. Leung, S. N. Georas, A. De Benedetto, and L. A. Beck, Activation of epidermal toll-like receptor 2 enhances tight junction function: implications for atopic dermatitis and skin barrier repair, J. Invest. Dermatol., 133(4), 988 (2013). https://doi.org/10.1038/jid.2012.437
  23. S. H. Lee, I. H. Bae, H. Choi, H. W. Choi, S. Oh, P. A. Marinho, D. J. Min, D. Y. Kim, T. R. Lee, C. S. Lee, and J. Lee, Ameliorating effect of dipotassium glycyrrhizinate on an IL-4-and IL-13-induced atopic dermatitis-like skin-equivalent model, Arch. Dermatol. Res, 311(2), 131 (2019). https://doi.org/10.1007/s00403-018-1883-z
  24. L. Su, L. Shen, D. R. Clayburgh, S. C. Nalle, E. A. Sullivan, J. B. Meddings, C. Abraham, and J. R. Turner, Targeted epithelial tight junction dysfunction causes immune activation and contributes to development of experimental colitis, Gastroenterology, 136(2), 551 (2009). https://doi.org/10.1053/j.gastro.2008.10.081
  25. A. Nusrat, J. R. Turner, and J. L. Madara, Molecular physiology and pathophysiology of tight junctions. IV. Regulation of tight junctions by extracellular stimuli: nutrients, cytokines, and immune cells, Am. J. Physiol. Gastrointest. Liver Physiol., 279(5), G851 (2000). https://doi.org/10.1152/ajpgi.2000.279.5.G851
  26. L. Shen, E. D. Black, E. D. Witkowski, W. I. Lencer, V. Guerriero, E. E. Schneeberger, and J. R. Turner, Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure, J. Cell. Sci., 119(Pt10), 2095 (2006). https://doi.org/10.1242/jcs.02915