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Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization

  • Ayed, Lamia (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy) ;
  • Zmantar, Tarek (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy) ;
  • Bayar, Sihem (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy) ;
  • Charef, Abdelkrim (Georesources Laboratory, Water Research and Technology Centre) ;
  • Achour, Sami (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy) ;
  • Mansour, Hedi Ben (Unit of Research Analysis and Processes Applied to the Environment UR17ES32 at the Higher Institute of Applied Sciences and Technology) ;
  • Mzoughi, Ridha El (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy)
  • Received : 2019.06.11
  • Accepted : 2019.08.21
  • Published : 2019.10.28

Abstract

Azo dyes are recalcitrant pollutants, which are toxic, carcinogenic, mutagenic and teratogenic, that constitute a significant burden to the environment. The decolorization and the mineralization efficiency of Remazol Brillant Orange 3R (RBO 3R) was studied using a probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 11.5 and temperature $25^{\circ}C$. The bio-decolorization process was further confirmed by FTIR and UV-Vis analysis. Under optimal conditions, the bacterial consortium was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37% at 750 ppm. Muliplex PCR technique was used to detect the Lactobacillus genes. Using phytotoxicity, cytotoxicity, mutagenicity and biototoxicity endpoints, toxicological studies of RBO 3R before and after biodegradation were examined. A toxicity assay signaled that biodegradation led to detoxification of RBO 3R dye.

Keywords

References

  1. Jayalakshmi R, Jeyanthi J. 2019. Simultaneous removal of binary dye from textile effluent using cobalt ferrite-alginate nano composite: performance and mechanism. Microchem. J. 145: 791-800. https://doi.org/10.1016/j.microc.2018.11.047
  2. Ayed L, Bakir K, Ben Mansour H, Hammami S, Cheref A, Bakhrouf A. 2017. In vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the "cna" adhesion gene in activated sludge. Microb. Pathog. 103: 29-39. https://doi.org/10.1016/j.micpath.2016.12.016
  3. Mahmoud MS, Mostafa MK, Mohamed SA, Sobhy NA, Nasr M. 2017. Bioremediation of red azo dye from aqueous solutions by Aspergillus niger strain isolated from textile wastewater. J. Environ. Chem. Eng. 5: 547-554. https://doi.org/10.1016/j.jece.2016.12.030
  4. Thung WE, Ong SA, Ho LN, Wong YS, Ridwan F, Lehl HK, et al. 2018. Biodegradation of acid orange 7 in a combined anaerobic-aerobic up-flow membrane-less microbial fuel cell: mechanism of biodegradation and electron transfer. Chem. Eng. J. 336: 397-405. https://doi.org/10.1016/j.cej.2017.12.028
  5. Ayed L, Khelifi E, Ben Jannet H, Miladi H, Cheref A, Achour S, et al. 2010c. Response surface methodology for decolorization of azo dye Methyl Orange by bacterial consortium: produced enzymes and metabolites characterization. Chem. Eng. J. 165: 200-208. https://doi.org/10.1016/j.cej.2010.09.018
  6. Ayed L, Achour S, Bakhrouf A. 2011a. Application of the Mixture Design to decolorize effluent textile wastewater using continuous stirred bed reactor. Water SA 37: 21-26.
  7. Ayed L, Mahdhi A, Cheref A, Bakhrouf A. 2011b. Decolorization and degradation of azo dye Methyl red by Sphingomonas paucimobilis. Desalination 274: 272-277. https://doi.org/10.1016/j.desal.2011.02.024
  8. Saratale RG, Saratale GD, Chang JS, Govindwar SP. 2011. Bacterial decolorization and degradation of azo dyes: a review. J. Taiwan Inst. Chem. Eng. 42: 138-157. https://doi.org/10.1016/j.jtice.2010.06.006
  9. Verma P, Madamwar D. 2003. Decolorization of synthetic dyes by anewly isolated strain of Serratia m arcescens. World J. Microbiol. Biotechnol. 19: 615-618. https://doi.org/10.1023/A:1025115801331
  10. Tastan BE, Ertugrul S, Donmez G. 2010. Effective bioremoval of reactive dye and heavy metals by Aspergillus versicolor. Bioresour. Technol. 101: 870-876. https://doi.org/10.1016/j.biortech.2009.08.099
  11. Aksu Z, Kilic NK, Ertugrul S, Donmez G. 2007. Inhibitory effects of chromium (VI) and Remazol Black B on chromium (VI) and dyestuff removals by Trametes versicolor. Enzyme Microb. Technol. 40: 1167-1174. https://doi.org/10.1016/j.enzmictec.2006.08.024
  12. Mahmood R, Sharif F, Ali S, Hayyat MU. 2014. Enhancing the decolorizing and. degradation ability of bacterial consortium isolated from textile effluent affected area and its application on seed germination. Sci. World J. 2015: 628195.
  13. Sadettin S, Donmez G. 2007. Simultaneous bioaccumulation of reactive dye and chromium (VI) by using thermophil Phormidium sp. Enzyme Microb. Techcnol. 41: 175-180. https://doi.org/10.1016/j.enzmictec.2006.12.015
  14. Karri RR, Tanzifi M, Yaraki MT, Sahu JN. 2018. Optimization and modeling of methyl orange adsorption onto polyaniline T nano-adsorbent through response surface methodology and differential evolution embedded neural network. J. Environ. Manag. 223: 517-529. https://doi.org/10.1016/j.jenvman.2018.06.027
  15. Kwon S, Yang EH, Yeon SW, Kang BH, Kim TY. 2004. Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA Hyuk. FEMS Microbiol. Lett. 239: 267-275. https://doi.org/10.1016/j.femsle.2004.08.049
  16. Jin C, Luo P, Zuo H, Chen J, Chen M, Wang W. 2012. Vibrio zhanjiangensis sp. nov. isolated from sea water of shrimp farming pond, Antonie Van Leeuwenhoek 101: 743-751. https://doi.org/10.1007/s10482-011-9688-5
  17. Seo MK, Park EJ, Ko SY, Choi EW, Kim S. 2018. Therapeutic effects of kefir grain Lactobacillus-derived extracellular vesicles in mice with 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease. J. Dairy Sci. 101: 8662-8671. https://doi.org/10.3168/jds.2018-15014
  18. Ayed L, Chaieb K, Cheref A, Bakhrouf A. 2009. Biodegradation of triphenylmethane dye Malachite Green by Sphingomonas paucimobilis. World J. Microbiol. Biotechnol. 25: 705-711. https://doi.org/10.1007/s11274-008-9941-x
  19. Ayed L, Cheriaa J, Laadhari N, Cheref A, Bakhrouf A. 2009. Biodegradation of Crystal Violet by an isolated Bacillus sp. Annals. Microbiol. 59: 267-272. https://doi.org/10.1007/BF03178327
  20. Ayed L, Achour S, Khelifi E, Cheref A, Bakhrouf A. 2010. Use of active consortia of constructed ternary bacterial cultures via mixture design for Congo Red decolorization enhancement. Chem. Eng. J. 162: 495-502. https://doi.org/10.1016/j.cej.2010.05.050
  21. Ayed L, Chaieb K, Cheref A, Bakhrouf A. 2010. Biodegradation of triphenylmethane dyes by Staphylococcus epidermidis. Desalination 260: 137-146. https://doi.org/10.1016/j.desal.2010.04.052
  22. Ayed L, Harbi B, Cheref A, Bakhrouf A, Achour S. 2010. Application of the Mixture Design to decolorize azo-dye Methyl Red to Design the Formulation of active consortia of bacterial cultures. Water Sci. Technol. 62: 2837-2845. https://doi.org/10.2166/wst.2010.709
  23. Ayed L, Bekir K., Achour S, Cheref A, Bakhrouf A. 2016. Exploring bioaugmentation strategies for azo dye CI Reactive Violet 5 decolourization using bacterial mixture: dye response surface methodology. Water. Environ. J. http://dx.doi.org/10.1111/2016.wej.12216.
  24. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113: 173-215. https://doi.org/10.1016/0165-1161(83)90010-9
  25. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  26. Vaigan AA, Moghaddam MRA, Hashemi HS. 2009. Effect of dye concentration on sequencing batch reactor performance. J. Environ. Health Sci. Eng. 6: 11-16.
  27. An SY, Min SK, Cha IH, Choi YL, Cho YS, Kim CH. 2002. Decolorization of triphenylmethane and azo dyes by Citrobacter sp. Biotechnol. Lett. 24: 1037-1040. https://doi.org/10.1023/A:1015610018103
  28. Ren SZ, Guo J, Zeng GQ, Sun GP. 2006. Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain. Appl. Microbiol. Biotechnol. 72: 1316-1321. https://doi.org/10.1007/s00253-006-0418-2
  29. Jadhav UU, Dawkar VV, Ghodake GS, Govindwar SP. 2008. Biodegradation of Direct Red 5B, a textile dye by newly isolated Comamonas sp. UVS. J. Hazard Mater. 158: 507-516. https://doi.org/10.1016/j.jhazmat.2008.01.099
  30. Kalyani DC, Telke AA, Dhanve RS, Jadhav JP. 2009. Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J. Hazard Mater. 163: 735-742. https://doi.org/10.1016/j.jhazmat.2008.07.020
  31. Wang H, Zheng XW, Su JQ, Tian Y, Xiong XJ, Zheng TL. 2009. Biological decolorization of the reactive dyes Reactive Black 5 by a novel isolated bacterial strain Enterobacter sp. EC3. J. Hazard. Mater. 171: 654-659. https://doi.org/10.1016/j.jhazmat.2009.06.050
  32. Kolekar YM, Kodam KM. 2012. Decolorization of textile dyes by Alishewanella sp. KMK6. Appl. Microbiol. Biotechnol. 95: 521-529. https://doi.org/10.1007/s00253-011-3698-0
  33. Zhang J, Zhou Q, Ou L. 2011. Kinetic, isotherm, and thermodynamic studies of the adsorption of methyl orange from aqueous solution by chitosan/alumina composite. J. Chem. Eng. Data 57: 412-419. https://doi.org/10.1021/je2009945
  34. Wang S , Yang B, Liu Y. 2017. Synthesis of a hierarchical SnS2 nanostructure for efficient adsorption of Rhodamine Bdye. J. Colloid Interface Sci. 507: 225-233. https://doi.org/10.1016/j.jcis.2017.07.053
  35. Phugare SS, Kalyani DC, Patil AV, Jadhav JP. 2011. Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. J. Hazard. Mater. 186: 713-723. https://doi.org/10.1016/j.jhazmat.2010.11.049
  36. Lade HS, Waghmode TR, Kadam AA, Govindwar SP. 2012. Enhanced biodegradation and detoxification of disperse azo dye Rubine GFL and textile industry effluent by defined fungalbacterial consortium. Int. Biodeterior. Biodegrad. 72: 94-107. https://doi.org/10.1016/j.ibiod.2012.06.001
  37. Ayed L, Ksibi I, Cheref A, Bakhrouf A. 2012. Response surface methodology for optimization of the treatment of textile wastewater by a novel bacterial consortium: enzymes and metabolites characterization. Afr. J. Biotechnol. 11: 12339-12355.
  38. Banerjee P, Barman SR, Sikdar D, Roy U, Mukhopadhyay A, Das P. 2017. Enhanced degradation of ternary dye effluent by developed bacterial consortium with RSM optimization, ANN modeling, and toxicity evaluation. Desalination Water Treat. 72: 249-265. https://doi.org/10.5004/dwt.2017.20422
  39. Raj A, Kumar S, Haq I, Singh SK. 2014. Bioremediation and toxicity reduction in pulp and paper mill effluent by newly isolated ligninolytic Paenibacillus sp. Ecol. Eng. 71: 355-362. https://doi.org/10.1016/j.ecoleng.2014.07.002
  40. Kumari V, Kumar S, Haq I, Yadav A, Singh VK, Ali Z, et al. 2014. Effect of tannery effluent toxicity on seed germination a-amylase activity and early seedling growth of mung bean (Vigna radiata) seeds. Int. J. Latest Res. Sci. Technol. 3: 165-170.
  41. Chen BY, Lin KW, Wang YM, Yen CY. 2009. Revealing interactive toxicity ofaromatic amines to azo dye decolorizer Aeromonas hydrophila. J. Hazard. Mater. 166: 187-194. https://doi.org/10.1016/j.jhazmat.2008.11.030
  42. Seesuriyachan P, Takenaka S, Kuntiya A, Klayraung S, Murakami S, Aoki K. 2007. Metabolism of azo dyes by Lactobacillus casei TISTR 1500 and effects of various factors on decolorization. Water Res. 41: 985-992. https://doi.org/10.1016/j.watres.2006.12.001
  43. Dhanve RS, Shedbalkar UU, Jadhav JP. 2008. Biodegradation of diazo reactive dye Navy Blue HE2R (Reactive Blue 172) by an isolated Exiguobacterium sp. RD3. Biotechnol. Bioprocess. Eng. 13: 53-60. https://doi.org/10.1007/s12257-007-0165-y
  44. Huang G, Wang W, Liu G. 2015. Simultaneous chromate reduction and azo dye decolourization by Lactobacillus paracase CL1107 isolated from deep sea sediment. J. Environ. Manage. 157: 297-302. https://doi.org/10.1016/j.jenvman.2015.04.031
  45. Marques RCP, Medeiros SRB, Dias CS, Barbosafilho JM, Agnez-Lima LF. 2003. Evaluation of the mutagenic potential of yangambin and of the hydroalcoholic extract of Ocotea duckei by the Ames test. Mutat. Res. 536: 117-120. https://doi.org/10.1016/S1383-5718(03)00040-8
  46. Nakayama T, Kimura T, Kodama MC. 1983. Generation of hydrogen peroxide and superoxide anions from active metabolites of naphthalamines and aminoazo dyes: its possible role in carcinogenesis. Carcinogenesis 4: 765-769. https://doi.org/10.1093/carcin/4.6.765
  47. Sweeney EA, Chipman JK, Forsythe SJ. 1994. Evidence for direct-acting oxidative genotoxicity by reduction products of azo dyes. Environ. Health Perspect. 102: 119-122.
  48. Erdogdular AO, Dilek KA. 2019. Bioremoval of reactive dye Remazol Navy by kefr grains. Appl. Biol Chem. 62: 22. https://doi.org/10.1186/s13765-019-0429-1
  49. Haq I, Raj A. 2018. Markandeya, Biodegradation of Azure-B dye by Serratia liquefaciens and its validation by phytotoxicity, genotoxicity and cytotoxicity studies. Chemosphere 196: 58-68. https://doi.org/10.1016/j.chemosphere.2017.12.153

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