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http://dx.doi.org/10.4014/jmb.1906.06019

Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization  

Ayed, Lamia (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy)
Zmantar, Tarek (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy)
Bayar, Sihem (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy)
Charef, Abdelkrim (Georesources Laboratory, Water Research and Technology Centre)
Achour, Sami (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy)
Mansour, Hedi Ben (Unit of Research Analysis and Processes Applied to the Environment UR17ES32 at the Higher Institute of Applied Sciences and Technology)
Mzoughi, Ridha El (Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy)
Publication Information
Journal of Microbiology and Biotechnology / v.29, no.10, 2019 , pp. 1629-1635 More about this Journal
Abstract
Azo dyes are recalcitrant pollutants, which are toxic, carcinogenic, mutagenic and teratogenic, that constitute a significant burden to the environment. The decolorization and the mineralization efficiency of Remazol Brillant Orange 3R (RBO 3R) was studied using a probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 11.5 and temperature $25^{\circ}C$. The bio-decolorization process was further confirmed by FTIR and UV-Vis analysis. Under optimal conditions, the bacterial consortium was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37% at 750 ppm. Muliplex PCR technique was used to detect the Lactobacillus genes. Using phytotoxicity, cytotoxicity, mutagenicity and biototoxicity endpoints, toxicological studies of RBO 3R before and after biodegradation were examined. A toxicity assay signaled that biodegradation led to detoxification of RBO 3R dye.
Keywords
Azo dye; RBO 3R; Lactobacillus; mutagenicity; biotoxicity; multiplex PCR;
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