Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay

  • Li, Taiying (Department of Applied Biology, Dong-A University) ;
  • Ji, Sungyeon (Department of Applied Biology, Dong-A University) ;
  • Jung, Boknam (Forest Insect Pests and Diseases Division, National Institute of Forest Science) ;
  • Kim, Bo Yeon (Department of Applied Biology, Dong-A University) ;
  • Lee, Kwang Sik (Department of Applied Biology, Dong-A University) ;
  • Seo, Mun Won (Ginseng Research Division, National Institute of Horticultural and Herbal Science) ;
  • Lee, Sung Woo (Ginseng Research Division, National Institute of Horticultural and Herbal Science) ;
  • Lee, Jungkwan (Department of Applied Biology, Dong-A University) ;
  • Lee, Seung-Ho (Ginseng Research Division, National Institute of Horticultural and Herbal Science)
  • Received : 2019.07.11
  • Accepted : 2019.08.30
  • Published : 2019.09.30
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Abstract

Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.

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References

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