Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Screening of Bacterial Strains for Alleviating Drought Stress in Chili Pepper Plants

고추 식물의 건조 스트레스 완화를 위한 미생물 선발

  • Kim, Sang Tae (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Yoo, Sung-Je (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Song, Jaekyeong (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Weon, Hang-Yeon (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Sang, Mee Kyung (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration)
  • 김상태 (농촌진흥청국립농업과학원농업생물부 농업미생물과) ;
  • 유성제 (농촌진흥청국립농업과학원농업생물부 농업미생물과) ;
  • 송재경 (농촌진흥청국립농업과학원농업생물부 농업미생물과) ;
  • 원항연 (농촌진흥청국립농업과학원농업생물부 농업미생물과) ;
  • 상미경 (농촌진흥청국립농업과학원농업생물부 농업미생물과)
  • Received : 2019.07.09
  • Accepted : 2019.09.02
  • Published : 2019.09.30
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Abstract

Drought stress is considered as one of major abiotic stresses; it leads to reduce plant growth and crop productivity. In this study, we selected bacterial strains for alleviating drought stress in chili pepper plants. As drought-tolerant bacteria, 28 among 447 strains were pre-selected by in vitro assays including growth in drought condition with polyethylene glycol and plant growth-promoting traits including production of 1-aminocyclopropane-1-carboxylate deaminase, indole-3-acetic acid and exopolysaccharide. Sequentially, 7 among pre-selected 28 strains were screened based on relative water content (RWC); GLC02 and KJ40, among seven strains were finally selected by RWC and malondialdehyde (MDA) in planta trials under an artificial drought condition by polyethylene glycol solution. Two strains GLC02 and KJ40 reduced drought stress in a natural drought condition as well as an artificial condition. Strains GLC02 or KJ40 increased shoot fresh weight, chlorophyll and stomatal conductance while they decreased MDA in chili pepper plants under a natural drought condition. However, two strains did not show biocontrol activity against diseases caused by Phytophthora capsici and Xanthomonas campestris pv. vesicatoria in chili pepper plants. Taken together, strains GLC02 or KJ40 can be used as bio-fertilizer for alleviation of drought stress in chili pepper plants.

식물 근권과 내생에서 분리한 447 균주 중 식물 생장 촉진특성과 건조 내성이 있는 28 균주를 일차적으로 스크리닝 하였으며, PEG에 의한 인위적 건조 스트레스 조건에서 잎의 상대수분함량과 MDA를 기반으로 GLC02와 KJ40을 선발하였다. 이 두 균주의 효과를 검증하기 위해 밭흙을 사용한 자연 건조에서 식물 포트 검정을 하였으며, 기공전도도와 지상부(줄기와 잎) 무게가 유의하게 증가한 반면 MDA가 감소하였다. 병 억제 효과에서는 GLC02를 처리할 경우 역병의 병진전도가 감소하였으며, KJ40을 처리할 경우 세균성 점무늬병에 대한 억제효과가 있었다. 이를 토대로, GLC02와 KJ40을 처리할 경우 건조 스트레스를 경감시켜주며 식물생장의 증진, 병 억제효과를 유도하여 생물비료의 소재로 사용할 수 있을 것으로 생각된다.

Keywords

References

  1. Anjum, S. A., Xie, X.-Y., Wang, L.-C., Saleem, M. F., Man, C. and Lei, W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. Afr. J. Agric. Res. 6: 2026-2032.
  2. Bao, A.-K., Wang, S.-M., Wu, G.-Q., Xi, J.-J., Zhang, J.-L. and Wang, C.-M. 2009. Overexpression of the Arabidopsis H+-PPase enhanced resistance the salt and drought stress in transgenic alfalfa (Medicago sativa L.). Plant Sci. 176: 232-240. https://doi.org/10.1016/j.plantsci.2008.10.009
  3. Barrow, J. R., Lucero, M. E., Reyes-Vera, I. and Havstad, K. M. 2008. Do symbiotic microbes have a role in regulating plant performance and response to stress? Commum. Integr. Biol. 1: 69-73. https://doi.org/10.4161/cib.1.1.6238
  4. Barrs, H. D. and Weatherley, P. E. 1962. A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust. J. Biol. Sci. 15: 413-428. https://doi.org/10.1071/BI9620413
  5. Fernandez, O., Theocharis, A., Bordiec, S., Feil, R., Jacquens, L., Clement, C. et al. 2012. Burkholderia phytofirmans PsJN acclimates grapevine to cold by modulating carbohydrate metabolism. Mol. Plant-Microbe Interact. 25: 496-504. https://doi.org/10.1094/MPMI-09-11-0245
  6. Kang, S.-M., Khan, A. L., Waqas, M., You, Y.-H., Kim, J.-H., Kim, J.-G. et al. 2014. Plant growth-promoting rhizobacteria reduce adverse effects of salinity and osmotic stress by regulating phytohormones and antioxidants in Cucumis sativus. J. Plant Interact. 9: 673-682. https://doi.org/10.1080/17429145.2014.894587
  7. Li, Y., Ye, W., Wang, M. and Yan, X. 2009. Climate change and drought: a risk assessment of crop-yield impacts. Clim. Res. 39: 31-46. https://doi.org/10.3354/cr00797
  8. Marasco, R., Rolli, E., Ettoumi, B., Vigani, G., Mapelli, F., Borin, S. et al. 2012. A drought resistance-promoting microbiome is selected by root system under desert farming. PLoS ONE 7: e48479. https://doi.org/10.1371/journal.pone.0048479
  9. Nair, A. S., Abraham, T. K. and Jaya, D. S. 2008. Studies on the changes in lipid peroxidation and antioxidants in drought stress induced cowpea (Vigna unguiculata L.) varieties. J. Environ. Biol 29: 689-691.
  10. Naveed, M., Hussain, M. B., Zahir, Z. A., Mitter, B. and Sessitsch, A. 2014. Drought stress amelioration in wheat through inoculation with Burkholderia phytofirmans strain PsJN. Plant Growth Regul. 73: 121-131. https://doi.org/10.1007/s10725-013-9874-8
  11. Penrose, D. M. and Glick, B. R. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol. Plant. 118: 10-15. https://doi.org/10.1034/j.1399-3054.2003.00086.x
  12. Sandhya, V., Ali, S. Z., Grover, M., Reddy, G. and Venkateswarlu, B. 2009. Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45. Biol. Fertil. Soils 46: 17-26. https://doi.org/10.1007/s00374-009-0401-z
  13. Sang, M. K., Chun, S.-C. and Kim, K. D. 2008. Biological control of Phytophthora blight of pepper by antagonistic rhizobacteria selected from a sequential screening procedure. Biol. Control 46: 424-433. https://doi.org/10.1016/j.biocontrol.2008.03.017
  14. Sang, M. K., Kim, J.-G. and Kim, K. D. 2010. Biocontrol activity and induction of systemic resistance in pepper by compost water extracts against Phytophthora capsici. Phytopathology 100: 774-783. https://doi.org/10.1094/PHYTO-100-8-0774
  15. Shin, D. J., Yoo, S.-J., Hong, J. K., Weon, H.-Y., Song, J. and Sang, M. K. 2019. Effect of Bacillus aryabhattai H26-2 and B. siamenisis H30-3 on growth promotion and alleviation of heat and drought stresses in Chinese cabbage. Plant Pathol. J. 35: 178-187. https://doi.org/10.5423/PPJ.NT.08.2018.0159
  16. Sofo, A., Dichio, B., Xiloyannis, C. and Masia, A. 2004. Effects of different irradiance levels on some antioxidant enzymes and on malondialdehyde content during rewatering in olive tree. Plant Sci. 166: 293-302. https://doi.org/10.1016/j.plantsci.2003.09.018
  17. Tallgren, A. H., Airaksinen, U., Von Weissenberg, R., Ojamo, H., Kuusisto, J. and Leisola, M. 1999. Exopolysaccharide-producing bacteria from sugar beets. Appl. Environ. Microbiol. 65: 862-864. https://doi.org/10.1128/AEM.65.2.862-864.1999
  18. Tank, N. and Saraf, M. 2010. Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plant. J. Plant Interact. 5: 51-58. https://doi.org/10.1080/17429140903125848
  19. Tiwari, S., Lata, C., Chauhan, P. S. and Nautiyal C. S. 2016. Pseudomonas putida attunes morphophysiological, biochemical and molecular responses in Cicer arietinum L. during drought stress and recovery. Plant Physiol. Biochem. 99: 108-117. https://doi.org/10.1016/j.plaphy.2015.11.001
  20. Yoo, S.-J., Shin, D. J., Weon, H.-Y., Song, J. and Sang, M. K. 2018. Selection of bacteria for enhancement of tolerance to salinity and temperature stresses in tomato plants. Korean J. Org. Agric. 26: 463-475. https://doi.org/10.11625/KJOA.2018.26.3.463
  21. Zhou, C., Ma, Z., Zhu, L., Xiao, X., Xie, Y., Zhu, J. et al. 2016. Rhizobacterial strain Bacillus megaterium BOFC15 induces cellular polyamine changes that improve plant growth and drought resistance. Int. J. Mol. Sci. 17: 976. https://doi.org/10.3390/ijms17060976
  22. Zhu, J.-K. 2002. Salt and drought stress signal transduction in plant. Annu. Rev. Plant Biol. 53: 247-273. https://doi.org/10.1146/annurev.arplant.53.091401.143329