• Proceedings of the IEEK Conference
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• 2000.07a
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• pp.482-485
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• 2000

A modified watershed transform is proposed which is context-free marker-controlled and minima imposition-free to reduce the over-segmentation and to speedup the transform. In contrast to the conventional methods in which a priori knowledge, such as flat zones, zones of homogeneous texture, and morphological distance, is required for marker extraction, context-free marker extraction is proposed by using the attention operator based on the GST (generalized symmetry transform). By using the context-free marker, the proposed watershed transform exploit marker-constrained labeling to speedup the computation and to reduce the over-segmentation by eliminating the unnecessary geodesic reconstruction such as the minima imposition and thereby eliminating the necessity of the post-processing of region merging. The simulation results show that the proposed method can extract context-free markers inside the objects from the complex background that includes multiple objects and efficiently reduces over-segmentation and computation time.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.212-219
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• 2010

Selectable marker gene systems are vital for the development of transgenic plants, but the presence of selectable marker genes encoding antibiotic or herbicide resistance in genetically modified plants poses a number of problems. A lot of research results and various techniques have been developed to produce marker-free GM plants. The aim of this review is to describe the principal methods used for eliminating selectable marker genes to generate marker-free GM plants, concentrating on the three significant methods(co-transformation, site-specific recombinase-mediated excision, non-selected transformation) in several marker-free techniques.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.1
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• pp.58-66
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• 2014

Numerical simulations for the flows containing free surface remain difficult problems because the drastic differences of physical properties of water and air, The difference of densities makes the solution instable in particular. For the stabilities of the solutions, the most typical methods to simulate free surface flows, such as Volume Of Fluid(VOF) and Level-Set(LS) methods, impose transient zones where the physical prosperities are continuously distributed. The thickness of the transient zone is the source of the numerical errors. The other side, marker-density method does not use such a transient zone. In the traditional marker-density method, however, the air velocities of free surface cells are extrapolated from the water velocity, and the pressures on the free surface are extrapolated from the air pressures for the stability of the solution. In this study, the marker-density method is modified for the decrease of such numerical errors. That is, the pressure on the free surface is determined to coincide with the pressure gradient terms of the governing equations, and the velocity of free surface cells are calculated with the governing equations. Two-dimensional steady spilling breakers behind of a submersed hydrofoil and three-dimensional spilling breaker near a wedge shaped ship model are simulated using INHAWAVE-II including the modified marker-density(MMD) method. The results are compared with the results of Fluent V6.3 including VOF method and several published research results.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.38 no.3
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• pp.237-246
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• 2001

A high speed context-free marker-controlled and minima imposition-free watershed transform is proposed for efficient multi-object detection and segmentation from a complex background. The context-free markers are extracted from a complex backgrounded multi-object image using a noise tolerant attention operator. These make marker-controlled watershed possible for the over-segmentation reduction without region merging. The proposed method presents a marker-constrained labeling that can speed up the segmentation of a marker-controlled watershed transform by eliminating the necessity of the minima imposition. Simulation results show that the proposed method can efficiently detects and segments multiple objects from a complex background while reducing over- segmentation and the computation time.

• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.113-121
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• 2001

The use of a marker gene in a transformation process aims to give a selective advantage to the transformed cells, allowing them to grow faster and better, and to kill the non-transformed cells. In general, the selective gene is introduced into plant genome along with the genes of interest. In some cases, the marker gene can be the gene of interest that will confer an agronomic characteristic, such as herbicide resistance. In this review we list and discuss the use of the most common selective marker genes on plant transformation and the effects of their respective selective agents. These genes could be divided in categories according their mode of action: genes that confer resistance to antibiotics and herbicides; and genes for positive selection. The contention of the marker gene flow through chloroplast transformation is further discussed. Moreover, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site specific recombination and intragenomic relocation of transgenes through transposable elements, are also reviewed.

• Natural Product Sciences
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• v.23 no.2
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• pp.75-83
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• 2017

The optimization and microwave assisted extraction of stem bark of Terminalia arjuna, quantitative estimation of the marker compounds arjunic acid and arjunolic acid using HPTLC and the evaluation of free radical scavenging activity has been performed in this study. The central composite design was used for optimization and the values of parameters for optimized batch of microwave assisted extraction were 1000 W (Power), 3 minutes (Time) and 1/120 (Solid/solvent ratio). The solvent system to carry out the HPTLC was toluene: acetic acid: ethyl acetate (5: 5: 0.5) and quantitative estimation was done using standard equations obtained from the marker compounds. The in-vitro free radical scavenging activity was performed spectrophotometrically using ascorbic acid as standard. The value of estimated percentage yield of arjunic acid and arjunolic acid was 1.42% and 1.52% which upon experimentation was obtained as 1.38% and 1.51% respectively. The DPPH assay of the different batches of microwave assisted extraction and marker compounds taken suggested that the marker compounds arjunic acid and the arjunolic acid were responsible for the free radical scavenging activity as the batch having the maximum percentage yield of the marker compounds showed best free radical scavenging effect as compared to standard ascorbic acid. The $IC_{50}$ value of the optimized batch was found to be 24.72 while that of the standard ascorbic acid was 29.83. Hence, the yield of arjunic acid and arjunolic acid has direct correlation with the free radical scavenging activity of stem bark extract of Terminalia arjuna and have potential to serve as active lead compounds for free radical scavenging activity.

• Journal of Ocean Engineering and Technology
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• v.14 no.3
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• pp.35-40
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• 2000

SMAC method is, one of the numerical simulation techniques, modified from the original MAC for the time-dependent variation of fluid flows. The Navier-Stokes equation for incompressible time-dependent viscous flow is applied and, also marker particles which move with the fluid are used. Two-dimensional numerical computations of fluid flow are carried out in a virtual water tank. This paper has shown very well the movements of marker particles using SMAC method.

• 한국전산유체공학회:학술대회논문집
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• 2004.03a
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• pp.119-125
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• 2004

Unsteady nonlinear wave motions on the free surface over a plane beach of constant slope are numerically simulated using a finite difference method in rectangular grid system. Two-dimensional Navier-Stokes equations and the continuity equation are used for the computations. Irregular leg lengths and stars are employed near the boundaries of body and free surface to satisfy the boundary conditions. Also, the free surface which consists of markers or segments is determined every time step with the satisfaction of kinematic and dynamic free surface conditions. Moreover, marker-density method is also adopted to allow plunging jets impinging on the free surface. The second-order Stokes wave theory and solitary wave theory are employed for the generation of waves on the inflow boundary. For the simulation of wave breaking phenomena, the computations are carried out with the plane beach of constant slope in surf zone. The results are compared with each other. The marker-density method is better then the hybrid method. Also they are compared with other existing experimental results. The Agreement between the experimental data and the computation results is good.

• 한국전산유체공학회:학술대회논문집
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• 2005.10a
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• pp.195-199
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• 2005

Wave breaking phenomenon near the fore body of a ship is numerically simulated. The ship advance with uniform velocity in calm water. For the simulation, incompressible Navier-Stokes equations and continuity equation are adopted as governing equations. The simulation is carried out in staggered variable mesh system with finite difference method. Marker and Cell(MAC) method and Marker-Density method are employed to track the free surface. Body boundary conditions are satisfied with the adoption of porosity method and no-slip condition on the hull surface. The ship model has a wedge type fore-body, and the computational domain is an appropriate region around the fore-body. The computation results are compared with some experimental results. Also the difference of the free surface tracking methods are discussed.

• International Journal of Naval Architecture and Ocean Engineering
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• v.7 no.3
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• pp.610-625
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• 2015

In this paper the wave run-up around a circular column in regular waves is numerically calculated to investigate the applicability of the Modified Marker-Density (MMD) method to prediction of wave run-up around an offshore platform. The MMD method is one of the methods to define the highly nonlinear free surface. The governing equations are the Navier-Stokes equations and the continuity equation which are computed in Cartesian grid system. To validate incident waves generated by numerical simulation, those are compared with the solutions of the Stokes $5^{th}$ order wave theory. The wave run-up simulations are performed varying the steepness and period of incident waves as referred experimental data. The numerical results are compared to the experimental data and the results show good agreements.