• 제목/요약/키워드: Xer/dif recombination system

검색결과 1건 처리시간 0.013초

One-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity

  • Jiang, Huofeng;Gao, Yamin;Zeng, Sheng;Wang, Shuai;Cao, Zhizhong;Tan, Yaoju;Yin, Huancai;Liu, Jianxiong;Zhang, Tianyu
    • Journal of Microbiology and Biotechnology
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    • 제29권9호
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    • pp.1488-1493
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    • 2019
  • The rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.