Journal of Marine Life Science (한국해양생명과학회지)

The Korean Society of Marine Life Science (한국해양생명과학회)
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Combined Toxicity in the Brackish Water Flea Diaphanosoma celebensis Exposed to Benzo[a]pyrene and Mercury

벤조피렌과 수은에 대한 기수산 물벼룩 Diaphanosoma celebensis의 복합 독성 영향

  • Yoo, Je-Won (Department of Life Science, College of Natural Sciences, Sangmyung University) ;
  • Cho, Hayoung (Department of Life Science, College of Natural Sciences, Sangmyung University) ;
  • Jeong, Jeon Min (Department of Life Science, College of Natural Sciences, Sangmyung University) ;
  • Lee, Young-Mi (Department of Life Science, College of Natural Sciences, Sangmyung University)
  • 유제원 (상명대학교 자연과학대학 생명과학과) ;
  • 조하영 (상명대학교 자연과학대학 생명과학과) ;
  • 전민정 (상명대학교 자연과학대학 생명과학과) ;
  • 이영미 (상명대학교 자연과학대학 생명과학과)
  • Received : 2019.09.20
  • Accepted : 2019.12.04
  • Published : 2019.12.13
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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are persistent and commonly detected in marine ecosystem. They coexist with a various contaminants including heavy metals in real environment, but most studies have been still focused on single effects of these contaminants to aquatic organisms. In this study, the single and combined effects of benzy[a]pyrene (B[a]P) and HgCl2 were investigated in the brackish water flea, Diaphanosoma celebensis. For combined effect study, three mixtures A (3:7), B (5:5), and C (7:3) of benzy[a]pyrene (B[a]P) and HgCl2 were determined by 48 h - LC50 values of single exposure. Combined effects of both chemicals were estimated by concentration addition (CA) model and independent action (IA) model. As results, 48 h - LC50 values of benzy[a]pyrene (B[a]P) and HgCl2 was 25.75 ㎍ l-1 and 3.6 ㎍ l-1, respectively. TU values of mixture A, B, and C were 1.06, 0.83, and 0.96, respectively, indicating additive effects of mixtures using CA model. This study will be helpful as basic data for understanding the combined effects of contaminants in marine environment.

다환방향족탄화수소류(PAHs)는 해양 환경에 오랜 기간 잔류하며 해양 생태계에 유해한 보편적인 환경 오염 물질이다. PAH류는 대부분 해양 환경에서 금속을 비롯한 다양한 오염 물질과 복합적으로 존재하지만 혼합 오염 물질이 개체에 미치는 영향에 대한 연구는 부족하다. 본 연구는 HgCl2와 Benzo[a]pyrene (B[a]P)이 해양 생물에 미치는 영향을 개체 수준에서 평가하기 위하여 기수산 물벼룩(Diaphanosoma celebensis)을 이용하여 단일 및 혼합 급성 독성 시험을 진행하였다. 혼합 독성 평가에 사용한 혼합물의 농도는 단일 독성 시험을 통하여 얻은 각각의 LC50을 기준으로 3:7 (Mixture A), 5:5 (Mixture B), 7:3 (Mixture C)의 비율로 혼합하여 결정하였다. 실험 결과 B[a]P와 HgCl2의 48 h - LC50은 각각 25.75 ㎍ l-1와 3.6 ㎍ l-1로 나타났다. 혼합 독성 평가 결과 Mixture A, B, C에서 TU는 각각 1.06, 0.83, 0.96로 나타났다. 이는 Mixture A, B, C가 CA 모델에 따라 작용함을 보여주며, HgCl2와 B[a]P의 상호작용을 연구하기 위해 CA 모델을 사용하는 것이 적합할 것으로 생각된다. 본 연구는 금속과 PAH류의 혼합 독성 평가를 위한 기초 자료가 될 수 있을 것이다.

Keywords

Acknowledgement

본 연구는 상명대학교 대학혁신지원사업 SM-URP 프로그램(2019-1)의 지원을 받아 수행하였음.

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