한국군사과학기술학회지 (Journal of the Korea Institute of Military Science and Technology)

  • 제22권4호
  • /
  • Pages.575-580
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  • 2019
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  • 1598-9127(pISSN)
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  • 2636-0640(eISSN)
한국군사과학기술학회 (The Korea Institute of Military Science and Technology)
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생물학작용제 검출 키트 개발 및 성능시험 연구

Development and Validation Study of Biological Agent Detection Kit

  • 조혜은 (국방과학연구소 제4기술연구본부)
  • Joe, Hae Eun (The 4th Research and Development Institute, Agency for Defense Development)
  • 투고 : 2019.05.13
  • 심사 : 2019.07.05
  • 발행 : 2019.08.05
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초록

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

키워드

GSGGBW_2019_v22n4_575_f0001.png 이미지

Fig. 1. Results of the real-time PCR amplification of genomic DNA(0.1 ng/μℓ)

GSGGBW_2019_v22n4_575_f0002.png 이미지

Fig. 2. Results of the real-time PCR amplification of dead cells(1×106 CFU/ml)

Table 1. The target gene of biological agents

GSGGBW_2019_v22n4_575_t0001.png 이미지

Table 2. List of reagent components

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Table 3. Reporter dye of target genes

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Table 4. The Ct value of genomic DNA amplification

GSGGBW_2019_v22n4_575_t0004.png 이미지

Table 5. The Ct value of dead cells amplification

GSGGBW_2019_v22n4_575_t0005.png 이미지

참고문헌

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