Bacillus subtilis가 함유된 목질계 인공토양의 물리·화학적 특성이 참싸리 생육에 미치는 영향

Effects of the Physicochemical Properties of Lignocellulosic Artificial Soil Containing Bacillus subtilis on the Growth of Lespedeza cyrtobotrya

Abstract

본 연구에서는 Bacilluls subtilis가 함유된 목질계 인공토양(피트모스:펄라이트:폭쇄처리된 참나무:미생물제형=3:1:3:3, w/w/w/w)을 제조 하였으며, 이를 대조구토양과 0%, 25%, 50%, 75% 및 100% 비율로 혼합하여 참싸리 종자 파종 후 소규모 야외포트 현장적용 하였다. 현장적용에 따른 대조구토양, 혼합토양(대조구토양+인공토양) 및 인공토양의 물리 화학적 특성을 분석하였고 참싸리 줄기 및 뿌리생장을 비교하였다. 혼합토양에서는 $0.04g/cm^3$ 이하의 용적밀도, 85 % 이상의 공극률, pH 4.3 - 4.7, 0.5 dS/m 이하의 전기전도도, 15.0 - 26.5의 탄질비, 23.6% - 43.2%의 유기물 함량 및 $157{\times}10^6CFU/g-624{\times}10^6CFU/g$의 미생물밀도를 나타냈다. 인공토양이 25% 및 50% 함유된 혼합토양에서는 참싸리 줄기 및 뿌리생장이 대조구토양보다 높게 나타났고 참싸리의 생장에 영향을 미치는 토양 인자는 용적밀도, 공극률, 수분보유력, 탄질비, 유기물함량 및 미생물함량으로 나타났다.

In this study, we prepared lignocellulosic artificial soil that contains Bacillus subtilis (peat moss/perlite/ steam-exploded oak wood/microbial culture = 3:1:3:3, w/w/w/w) for use in the restoration of damaged soil areas. The prepared lignocellulosic artificial soil was mixed with soil at ratios of 0%, 25%, 50%, 75%, and 100%. These mixed soils were then applied to fields, and the resultant physicochemical properties and their effects on the plant growth of Lespedeza cyrtobotrya were observed. The mixture of the prepared artificial soils (mixed at ratios of 25%-100%) with soil had a bulk densities of <$0.04g/cm^3$, porosities of >85%, pH values between 4.3 and 4.7, electrical conductivities of <0.5 dS/m, C/N ratios between 15.0 and 26.5, organic matter content between 23.6% and 43.2%, and bacterial densities between $157{\times}10^6$ and $624{\times}10^6CFU/g$. In addition, the prepared artificial soils mixed with soil at ratios of 25%-50% exhibited higher plant growth rates for L. cyrtobotrya compared with the control. Overall, we identified positive correlations between the plant growth of L. cyrtobotrya and soil bulk density, porosity, water-holding capacity, C/N ratio, organic matter, and bacterial densities.

Fig. 1. Effect of mixing ratio of artificial soil with soil on growth of Lespedeza cyrtobotrya.

Table 1. Physical properties on mixing ratio of artificial soil with soil.

Table 2. Chemical properties on mixing ratio of artificial soil with soil

Table 3. Change of bacteria density in mixing ratio of artificial soil with soil on field application

Table 4. The values of correlation coefficient (r)¹⁾ between physicochemical properties of artificial soil and growth characteristics of Lespedeza cyrtobotrya