Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Study for Whitening Activity of Mixture of Arbutin and Oil Soluble Licorice Extract

알부틴과 유용성감초 추출물 혼합물에 의한 미백활성 연구

  • Jang, Hye In (Department. of Oriental Medical and Herbal Cosmetic Sciences, Semyung University)
  • 장혜인 (세명대학교 화장품뷰티생명공학부)
  • Received : 2019.06.09
  • Accepted : 2019.06.28
  • Published : 2019.06.30
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Abstract

The aim of this study was to investigate whitening effect of combination between arbutine and oil soluble licorice extract. Inhibitory effects of arbutin and oil soluble licorice extract against tyrosinase activity and melanogenesis in B16 melanoma cells were assessed in vitro to determine whitening effect. MTT assay with B16 melanoma cells showed that mixture (arbutin and oil soluble) was not each concentration. Both oil soluble licorice extract and arbutin induced dose-dependent inhibition of mushroom tyrosinase activity. Various concentrations (oil soluble extract : arbutin = 1:1. 1:1.5, 1:2, 1:2.5, 1:5) of mixtures also significantly inhibited tyrosinase activity in B16 melanoma cells by 40-51%. In addition, the mixtures reduced the melanin contents of B16 melanoma cells by more than 50% at each concentration. These results suggest that mixtures of arbutin and oil soluble licorice extract are very effective whitening ingredients.

본 연구의 목적은 arbutine과 유용성감초추출물 혼합물의 미백 효과를 조사하는 것이다. B16 melanoma 세포에서 알부틴 및 유용성 감초 추출물의 tyrosinase 활성과 멜라닌 생성 억제 효과를 시험관내에서 평가하여 미백 효과를 측정 하였다. B16 흑색 종 세포를 이용한 MTT 분석은 혼합물 (알부틴과 유용성감초추출물)이 세포독성이 없음을 확인하였다. 유용성 감초 추출물과 알부틴은 모두 mushroom tyrosinase 활성이 농도 의존적 효과를 보였다. 혼합물은 다양한 농도 (유용성감초추출물 : 알부틴 = 1 : 1, 1 : 1.5, 1 : 2, 1 : 2.5, 1 : 5)에서 B16 melanoma 세포에서 40-51 %의 tyrosinase 활성을 유의하게 억제하였다. 또한, 시험한 모든 혼합물은 B16 melanoma cell의 멜라닌 함량을 50 % 이상 감소시켰다. 이러한 결과는 알부틴과 유용성감초추출물 혼합사용 시 미백 활성에 효과적임을 시사하는 바이다.

Keywords

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Fig. 1. Structures of Arbutin and Glabridin

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Fig. 2. Effect of Arbutin(a), Oil soluble licorice(b) and mixture(c) on the viability of B16 melanoma cells by MTT assay.

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Fig. 3. Inhibitory effects of Arbutin(a), Oli soluble licorice(b) and Mixture(c) on the activity of mushroom tyrosinase.

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Fig. 4. Inhibitory effects of Arbutin(a), Oli soluble licorice(b) and Mixture(c) on the tyrosinase activity in B16 melanoma cells.

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Fig. 5. Inhibitory effects of Arbutin(a), Oli soluble licorice extract(b) and Mixture(c) on the melanin synthesis in B16 melanoma cells.

Table 1. Ingredients and content of whitening products

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