한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제36권2호
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  • Pages.383-393
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  • 2019
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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울금(Curcuma longa L.) 섭취가 당뇨성 흰쥐의 크레아티닌 및 간 기능 효소 활성에 미치는 영향

4Effects of Turmeric (Curcuma longa L.) Supplementation on Creatinine and Hepatic Functional Enzyme Activities in Streptozotocin-induced Diabetic Rats

  • Oh, Da-Young (Department of Food Science and Technology, Pusan National University) ;
  • Lee, Young-Geun (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Dong-Seob (Department of Food Science and Technology, Pusan National University) ;
  • Chung, Hun-Sik (Department of Food Science and Technology, Pusan National University) ;
  • Kang, Dong-Soo (Department of Marine Bio Food Science, Chonnam National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • 투고 : 2019.05.01
  • 심사 : 2019.06.12
  • 발행 : 2019.06.30
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초록

Streptozotocin (STZ, 45 mg/kg body weight)으로 유도된 Sprague Dawley (SD)계 수컷 흰쥐에 5% 울금을 섭취시켰을 때, 혈청 크레아티닌(creatinine) 및 blood urea nitrogen (BUN)의 농도와 alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aminotransferase (AST, ALT), 카탈라아제(catalase), 아밀라아제(amylase) 및 리파아제(lipase) 활성에 미치는 영향을 알아보기 위해 본 실험을 수행한 결과, 기본식이를 급여시킨 대조군(control)인 정상군은 BD군, 기본식이 실험군에 5% 울금 급여군(BT군), 당뇨 유발 실험군(BS군)인 질환 모델 대조군(control-diabetic)과 질환 실험군(diabetic, BS군)에 5% 울금을 급여시킨 실험군(ST군)으로 나누었다. 흰쥐의 혈청 크레아티닌 및 BUN 농도와 ALP, AST 및 ALT 활성은 유사한 경향으로 5% 울금의 급여가 감소시킨 것으로 확인되었다. SD계 수컷 흰쥐의 혈청 LDH 활성은 BS군에서 유의적인 차이를 나타내며 높은 활성을 보였으나(p<0.05), 5% 울금의 급여가 감소 효과를 나타내었다(p<0.05). 카탈라아제 활성은 BS군에서 유의적으로 낮은 활성을 나타내었고, ST군에서 활성을 증가시켰다(p<0.05). 혈청 아밀라아제 및 리파아제의 활성은 5% 울금의 급여가 감소시킨 것으로 확인되었다. 본 연구 결과, SD계 수컷 흰쥐를 통한 in vivo 실험에서 5% 울금의 섭취가 당뇨, 내인성 항산화 효소 및 간 기능 개선에 효과가 있는 것으로 나타났다.

The purpose of this study was to investigate the improvement effect of turmeric (Curcuma longa L.) on the hepatic functional enzyme and catalase activity of streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley (SD) male rats were divided into four groups (n=6), and fed experimental diets containing turmeric meal [basal diet+5% turmeric (BT), basal diet+STZ+5% turmeric (ST)], and control (basal diet, BD), BS groups (basal diet+STZ). Serum concentrations of creatinine and blood urea nitrogen (BUN) were significantly decreased (p<0.05) by 5% turmeric supplementation diet. The activities of akaline phosphatase (ALP), lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT), amylase and lipase were decreased in the BD, BT and ST group than BS group. The catalase (CAT) activity was significantly increased (p<0.05) in turmeric supplementation diet (BT, ST group) than diabetic group (BS). Furthermore, the activities of amylase and lipase in the sera of turmeric diet group were significantly decreased (p<0.05). In vivo experiments with diabetic rats showed that ingestion of turmeric supplementation diet were effective in creatinine concentration, and hepatic functional enzyme activities.

키워드

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Fig. 1. Effects of turmeric (Curcuma longa L.) on serum alkaline phosphatase (ALP) activity of STZ-induced diabetic rats.

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Fig. 2. Effects of turmeric (Curcuma longa L.) on serum lactate dehydrogenase (LDH) activity of STZ-induced diabetic rats.

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Fig. 3. Effects of turmeric (Curcuma longa L.) on serum aspartate and alanine aminotransferase (AST and ALT) activity of STZ-induced diabetic rats.

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Fig. 4. Effects of turmeric (Curcuma longa L.) on serum catalase activity of STZ-induced diabetic rats.

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Fig. 5. Effects of turmeric (Curcuma longa L.) on amylase activity of STZ-induced diabetic rats.

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Fig. 6. Effects of turmeric (Curcuma longa L.) on serum lipase activity of STZ-induced diabetic rats.

Table 1. Compositions of experimental diet and groups

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Table 2. Effects of turmeric (Curcuma longa L.) on serum creatinine and BUN concentration of STZ-induced diabetic rats

HGOHBI_2019_v36n2_383_t0002.png 이미지

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